FlyBase Recombinant Construct Report: P{Tub67C-Tau-GFP}

General Information

Symbol

P{Tub67C-Tau-GFP}

FlyBase ID

FBtp0008375

Feature type

transgenic_transposable_element

Component Allele(s)

Avic\GFP^{αTub67C.S65T.I167T.Tag:MT(btau)}

Also Known As

Tau-GFP, Tau:GFP

Size

Expression Data

GFP reporter

Associated insertion(s)

2 available

Molecular map

Description and Uses

Construct components

Component

Use(s)

Marker(s)

w^+mC

Component allele Avic\GFP^{αTub67C.S65T.I167T.Tag:MT(btau)}

Transgenic product class

Component

Use(s)

Regulatory region(s)

αTub67C

Encoded product / tool

GFP(S65T.I167T)

green fluorescent protein

Tagged with

Tag:MT(btau)

microtubule protein localization tag

Description

αTub67C sequences (including approximately 1.5kb upstream sequences, the initiation codon, a 487bp intron and the 2nd to 9th amino acids of αTub67C) drive expression of the first 382 amino acids of Btau\MAPT fused to the GFP(S65T.I167T) fluorescent protein.

(Micklem et al., 1997)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Avic\GFP^{αTub67C.S65T.I167T.Tag:MT(btau)}

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female & oogenesis

oocyte

• microtubule

(Micklem et al., 1997)

adult stage & oogenesis

oocyte

(Micklem et al., 1997)

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage & oogenesis

oocyte

(Micklem et al., 1997)

Additional Information

Statement

Reference

In oocyte, observed fluorescence of all microtubules. The fusion protein used as marker for microtubule network within the oocyte.

(Micklem et al., 1997)

Used as marker for microtubule network within the oocyte.

(Micklem et al., 1997)

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

construct_comment: Allows visualization of oocyte microtubules in living tissue.

(Micklem et al., 1997)

Stocks (0)

Stocks containing insertion(s) of this recombinant construct

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

P[w+; Tau-GFP]

(Huynh et al., 2004)

P{Tub67C-Tau-GFP}

(FlyBase, 1996-)

P{btau^{αTub67C.T:GFP-S65T.I167T}}

(FlyBase, 1996-)

P{w⁺, mat-tub-α4:TauGFP}

(Loiseau et al., 2010)

Tau-GFP

(Trovisco et al., 2016, Morais-de-Sá et al., 2014, Dubin-Bar et al., 2011, Clark et al., 2007, Abdu et al., 2006, Januschke et al., 2006, Shapiro and Anderson, 2006, Weil et al., 2006, Macdougall et al., 2003)

Tau:GFP

(Jankovics et al., 2002, Jankovics et al., 2001, Shulman et al., 2000)

TauGFP

(MacDougall et al., 2001)

TauGFP24.1

(Dollar et al., 2002)

Tub-TauGFP

(Guichet et al., 2001)

Tub67CMatpolyA:Tau:GFP

(Benton et al., 2002)

UASp-Tau:GFP

(Zimyanin et al., 2007, Zimyanin et al., 2007)

tau-GFP

(Gergely et al., 2000, Wakefield et al., 2000, Robinson et al., 1999)

α4 tubulin-tau-GFP

(Brand, 1999)

Secondary FlyBase IDs

References (27)

Report Sections

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