FB2024_03 , released June 25, 2024
Recombinant Construct: P{ovo.stop2b-lacZ}
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General Information
Symbol
P{ovo.stop2b-lacZ}
FlyBase ID
FBtp0015825
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Construct components
Component allele ovostop2b.lacZ
Transgenic product class
Component
Use(s)
Regulatory region(s)
Encoded product / tool
Description

7kb SalI-HindIII genomic fragment containing ovo, with a stop codon introduced at position 3960 (60 residues after the beginning of exon 2b). In addition, the ovo open reading frame is tagged at the C-terminal end (two codons upstream of the stop codon) with lacZ. The presence of the stop codon in exon 2b means that only mRNAs that have spliced out this exon give rise to a ovo ::lacZ fusion protein.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{ovo.stop2b-lacZ}
ovo(stop2b)-lacZ
Secondary FlyBase IDs
    References (2)