FlyBase Recombinant Construct Report: P{btl-moe.mRFP1}

General Information

Symbol

P{btl-moe.mRFP1}

FlyBase ID

FBtp0020226

Feature type

transgenic_transposable_element

Component Allele(s)

Moe^btl.mRFP1

Also Known As

btlenhancer-mRFP1moe, btl-RFP-moe

Size

Expression Data

Associated insertion(s)

1 available

Molecular map

Description and Uses

Construct components

Component

Use(s)

Marker(s)

w^+mC

Component allele Moe^btl.mRFP1

Transgenic product class

Component

Use(s)

Regulatory region(s)

btl

Encoded product / tool

Moe

Tagged with

mRFP1

red fluorescent protein

Description

The "B123" btl upstream fragment drives expression of the actin binding domain of Moe which is tagged at the N-terminal end with mRFP1.

(Ribeiro et al., 2004)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Moe^btl.mRFP1

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter or direct label

Stage

Tissue/Position (including subcellular localization)

Reference

first instar larval stage -- third instar larval stage

adult tracheal air sac

(Cabernard and Affolter, 2005)

second instar larval stage

tracheal metamere 5 -- tracheal metamere 10

(Chen and Krasnow, 2014)

second instar larval stage -- prepupal stage P4

imaginal tracheal precursor

(Chen and Krasnow, 2014)

Additional Information

Statement

Reference

Imaginal tracheal progenitors proliferating in the spiracular branch niche begin to express Moe^btl.mRFP1 as they become activated. Moe^btl.mRFP1 expression increases as progenitors leave the niche and migrate dorsally along transverse connective branches.

(Chen and Krasnow, 2014)

Marker for

Reflects expression of

Moe

(Chen and Krasnow, 2014)

btl

(Cabernard and Affolter, 2005)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (2)

Stocks containing insertion(s) of this recombinant construct

Bloomington

64233

w^*; P{vvl-FLP1.M}2/CyO; P{btl-moe.mRFP1}3

41803

y¹ w^*; wg^Sp-1/CyO; P{btl-moe.mRFP1}3, P{GAL4-btl.S}3-1, P{UAS-mCD8::GFP.L}LL6/TM6B, Tb¹

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

(RFP)-Moesin

(Förster and Luschnig, 2012)

P[B123]-RFP-moe

(Li et al., 2022)

P{btl-moe.mRFP1}

(Miao and Hayashi, 2015, FlyBase, 1996-)

RFP-Moe

(Förster and Luschnig, 2012)

btl-RFP-moe

(Miao and Hayashi, 2016, Chen and Krasnow, 2014)

btl-mRFP-Moe

(Förster and Luschnig, 2012, Forster et al., 2010)

btl-mRFP1moe

(Du et al., 2018)

btl-moe-mRFP

(Sánchez-Higueras and Hombría, 2016)

btl::moeRFP

(Ricolo and Araujo, 2020)

btlRFPmoe

(Araújo and Casanova, 2011)

btlenhancer-mRFP1-moe

(Ribeiro et al., 2004)

btlenhancer-mRFP1moe

(Wang et al., 2010, Chanut-Delalande et al., 2007, Cabernard and Affolter, 2005)

btlenhancer-mRFP::moe

(Caussinus et al., 2008)

btlmoeRFP

(Butí et al., 2014)

mRFP::moe

(Caussinus et al., 2008)

Secondary FlyBase IDs

References (19)

Report Sections

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