FlyBase Recombinant Construct Report: P{Hand-EGFP.C}

FB2024_03 , released June 25, 2024

FB2024_03 , released June 25, 2024

Recombinant Construct: P{Hand-EGFP.C}

General Information

Symbol

P{Hand-EGFP.C}

FlyBase ID

FBtp0022116

Feature type

transgenic_transposable_element

Component Allele(s)

Avic\GFP^EGFP.HandC

Also Known As

handC-GFP, hand-GFP, hand-C-GFP

Size

Expression Data

GFP reporter

Associated insertion(s)

Molecular map

Description and Uses

Construct components

Component allele Avic\GFP^EGFP.HandC

Transgenic product class

Component

Use(s)

Regulatory region(s)

Encoded product / tool

green fluorescent protein

Tagged with

nuclear protein localization tag

Description

1611bp of Hand genomic sequence (+827 to +2438), incorporating intron 3 is fused upstream of EGFP tagged with a nuclear localization signal (Tag:NLS(tra)).

(Sellin et al., 2006)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Avic\GFP^EGFP.HandC

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11

circular visceral muscle primordium

(Sellin et al., 2006)

embryonic stage 11 -- 13

visceral mesoderm

(Sellin et al., 2006)

embryonic stage 12 -- 13

embryonic cardioblast

(Sellin et al., 2006)

pericardial cell primordium

(Sellin et al., 2006)

embryonic stage 12 -- 14

heart primordium

(Sellin et al., 2006)

embryonic stage 14 -- 17

embryonic/larval cardial cell

(Sellin et al., 2006)

embryonic/larval pericardial cell

(Sellin et al., 2006)

circular visceral muscle fiber

(Sellin et al., 2006)

embryonic/larval heart

(Sellin et al., 2006)

embryonic stage 15

embryonic/larval garland cell

(Sellin et al., 2006)

embryonic stage 15 -- 17

embryonic/larval heart

(Sellin et al., 2006)

embryonic stage 16 -- 17

embryonic/larval lymph gland

(Sellin et al., 2006)

scutellar pulsatile organ

Comment: precursor

(Tögel et al., 2008)

scutellar pulsatile organ

Comment: precursor

(Tögel et al., 2008)

first instar larval stage

embryonic/larval dorsal vessel

(Sellin et al., 2006)

embryonic/larval garland cell

(Sellin et al., 2006)

embryonic/larval cardial cell

(Sellin et al., 2006)

embryonic/larval pericardial cell

(Sellin et al., 2006)

embryonic/larval lymph gland

(Sellin et al., 2006)

circular visceral muscle fiber

(Sellin et al., 2006)

embryonic/larval heart

(Sellin et al., 2006)

first instar larval stage -- third instar larval stage

embryonic/larval pericardial cell

(Das et al., 2008)

embryonic/larval cardial cell

(Das et al., 2008)

second instar larval stage

embryonic/larval cardial cell

(Sellin et al., 2006)

embryonic/larval pericardial cell

(Sellin et al., 2006)

embryonic/larval garland cell

(Sellin et al., 2006)

embryonic/larval dorsal vessel

(Sellin et al., 2006)

circular visceral muscle fiber

(Sellin et al., 2006)

embryonic/larval heart

(Sellin et al., 2006)

third instar larval stage

embryonic/larval cardial cell

(Sellin et al., 2006)

embryonic/larval pericardial cell

(Sellin et al., 2006)

embryonic/larval garland cell

(Sellin et al., 2006)

embryonic/larval dorsal vessel

(Sellin et al., 2006)

circular visceral muscle fiber

(Sellin et al., 2006)

embryonic/larval heart

(Sellin et al., 2006)

(Sellin et al., 2006)

pericardial cell

(Sellin et al., 2006)

scutellar pulsatile organ

(Tögel et al., 2008, Sellin et al., 2006)

adult dorsal vessel

(Sellin et al., 2006)

(Sellin et al., 2006)

(Sellin et al., 2006)

(Sellin et al., 2006)

pericardial cell

(Sellin et al., 2006)

scutellar pulsatile organ

(Sellin et al., 2006)

(Sellin et al., 2006)

circular visceral muscle fiber

(Sellin et al., 2006)

Additional Information

Statement

Reference

Marker for

Reflects expression of

(Das et al., 2008, Sellin et al., 2006)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (1)

Stocks containing insertion(s) of this recombinant construct

600222

P{Hand-EGFP.C}4.1, w^*

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (12)

Reported As

Symbol Synonym

C

(Sellin et al., 2006)

C-GFP

(Sellin et al., 2006)

HandC-GFP

(Tian et al., 2023, Mendoza-Garcia et al., 2021, Bataillé et al., 2020)

P{Hand-EGFP.C}

(FlyBase, 1996-)

P{Hand.1611.EGFP.Stinger}

(FlyBase, 1992-)

hand 3^rd intron 1-1612::GFP

(Popichenko et al., 2007)

hand C-GFP

(Sellin et al., 2006)

hand-C-GFP

(Das et al., 2008, Popichenko et al., 2007)

hand-GFP

(Schaub et al., 2019, Popichenko et al., 2007, Albrecht et al., 2006)

hand>GFP

(Albrecht et al., 2006)

handC-GFP

(Meyer et al., 2023, Drechsler et al., 2013, Popichenko et al., 2013)

handC3.1-GFP

(Jammrath et al., 2020)

Secondary FlyBase IDs

References (17)

Report Sections