FlyBase Recombinant Construct Report: PBac{I-SceI(FRT.Rab32-GAL4.ex1(loxP.3xP3-RFP))}

Contains a genomic fragment centred on Rab32 in which the coding region of the Rab32 first exon has been replaced with the GAL4 driver coding region and a loxP cassette containing Disc\RFP^3xP3.PC and the kanamycin resistance gene.

(Chan et al., 2011)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Scer\GAL4^Rab32.ex1

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

neuron | subset of embryonic/larval brain

(Chan et al., 2011)

glial cell | subset of embryonic/larval brain

(Chan et al., 2011)

neuron | subset of larval ventral nerve cord

(Chan et al., 2011)

Bolwig organ | restricted

(Jin et al., 2012)

leg disc | restricted

(Jin et al., 2012)

pupal stage P6 -- P7

neuron | subset of pupal brain

Comment: 30-40h APF

(Chan et al., 2011)

adult stage

neuron | subset of adult brain

(Chan et al., 2011)

neuron of adult optic lobe | faint

(Jin et al., 2012)

adult insulin secreting cell of pars intercerebralis

(Jin et al., 2012)

neuron of lamina

(Jin et al., 2012)

Additional Information

Statement

Reference

ScerGAL4^ltd.ex1 drives expression in many neurons and possibly glia in the larval brain. In pupal and adult brain, this expression is observed in some neurons in the central brain.

(Chan et al., 2011)

Marker for

Reflects expression of

Rab32

(Jin et al., 2012, Chan et al., 2011)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

The PBac{I-SceI(FRT.Rab32-GAL4.ex1(loxP.3xP3-RFP))} vector can be used both as a driver expressing Scer\GAL4 under the control of the ltd regulatory sequences present in the construct and also as a donor construct for ends-out homologous recombination at the endogenous ltd locus.

(Chan et al., 2011)

Stocks (1)

Stocks containing insertion(s) of this recombinant construct

Bloomington

51598

y¹ w^*; PBac{I-SceI(FRT.Rab32-GAL4.ex1(loxP.3xP3-RFP))}VK00033

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

PBac{I-SceI(FRT.Rab32-GAL4.ex1(loxP.3xP3-RFP))}

(FlyBase, 1996-)