Contains a genomic fragment centred on Rab1 in which part of the Rab1 open reading frame (extending from the ATG to the end of the ATG-containing exon) has been replaced with the GAL4 driver coding region and a loxP cassette containing Disc\RFP3xP3.PC and the kanamycin resistance gene.
Comment: 30-40h APF
Comment: 30-40h APF
Comment: 30-40h APF
ScerGAL4Rab1.ATG drives expression in the larval brain neurons, as well as in the eye, wing and leg imaginal discs. In the pupal and adult brain, expression is ubiquitous. Expression in the adult mushroom body is stronger than in the pupal one.
The PBac{I-SceI(FRT.Rab1-GAL4.ATG(loxP.3xP3-RFP))} vector can be used both as a driver expressing Scer\GAL4 under the control of the Rab1 regulatory sequences present in the construct and also as a donor construct for ends-out homologous recombination at the endogenous Rab1 locus.