FlyBase Recombinant Construct Report: P{nanos-MCP-mCherry-FLAG}

nos promoter sequences drive expression of a Tag:MCP-mCherry fusion protein. A Tag:NLS(SV40-largeT) nuclear localization signal is present at the N-terminal end of the open reading frame (ORF) and a Tag:FLAG epitope is present at the C-terminal end of the ORF. The portion of the fs(1)K10 3'UTR which stabilises germline transcripts and restricts them to nurse cells is also present. FlyBase curator comment: in the presence of a mRNA tagged with Tag:MS2 (an RNA stem loop to which Tag:MCP binds), the Tag:MCP-mCherry fusion protein can bind to this tagged mRNA, allowing its localization within the cell to be studied. In the absence of a Tag:MS2-tagged mRNA (or when unbound), the Tag:MCP-mCherry fusion protein will be targeted to the nucleus due to the presence of the Tag:NLS(SV40-largeT) tag.

(Hayashi et al., 2014)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Disc\RFP^{mCherry.nanos.Tag:MCP,Tag:FLAG,Tag:NLS(SV40-largeT)}

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (0)

Stocks containing insertion(s) of this recombinant construct

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

P{nanos-MCP-mCherry-FLAG}

(FlyBase, 1996-)

P{nos-MCP-mCherry-FLAG}

(FlyBase, 1992-)

nanos::MCP-mCherry

(Hayashi et al., 2014)

Secondary FlyBase IDs

References (3)

Report Sections

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