Mi{Trojan-p65AD.2} represents a transgenic construct generated in vivo by using phiC31:int-mediated recombination to replace the attP cassette of a Mi{MIC} element insertion with an intron phase 2 'Trojan p65AD' cassette from the pBS-KS-attB2-SA(2)-T2A-p65AD-Hsp70 plasmid. The Trojan p65AD cassette consists of a splice acceptor site followed by the T2A peptide, sequence encoding a p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain) and an Hsp70 transcription termination signal. Integration of the cassette into a Mi{MIC} insertion in a coding intron (with the same phase) of a native Drosophila gene of interest will result in the cassette behaving as a 'Trojan' exon: the splice acceptor site ensures that the T2A-p65(AD)::Zip+ open reading frame is incorporated into the mRNA of the native Drosophila gene, while the T2A sequence truncates the native gene product and promotes the separate translation of the p65(AD)::Zip+ open reading frame. Thus the p65(AD)::Zip+ hemidriver should be expressed under the control of the regulatory sequences of the native Drosophila gene of interest in the resulting fly line.
One of 3 essentially identical transgenic constructs (Mi{Trojan-p65AD.0}, Mi{Trojan-p65AD.1} and Mi{Trojan-p65AD.2}) that vary only in the phase of the Trojan p65AD cassette.