FlyBase Recombinant Construct Report: P{R11F03-GAL4.DBD}

FB2024_03 , released June 25, 2024

FB2024_03 , released June 25, 2024

Recombinant Construct: P{R11F03-GAL4.DBD}

General Information

Symbol

P{R11F03-GAL4.DBD}

FlyBase ID

FBtp0099454

Feature type

transgenic_transposable_element

Component Allele(s)

Scer\GAL4^DBD.R11F03

Also Known As

R11F03

Size

Expression Data

GAL4 reporter/driver

Associated insertion(s)

Molecular map

Description and Uses

Construct components

Component allele Scer\GAL4^DBD.R11F03

Transgenic product class

Component

Use(s)

Regulatory region(s)

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Description

The R11F03 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Scer\GAL4^DBD.R11F03

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

first instar larval stage -- third instar larval stage

larval mushroom body output neuron h1

Comment: when combined with P{R67B01-p65.AD} (combination referred to as 'SS00894'), MBON-h1/h2 were indistinguishable

(Eschbach et al., 2021)

larval mushroom body output neuron h2

Comment: when combined with P{R67B01-p65.AD} (combination referred to as 'SS00894'), MBON-h1/h2 were indistinguishable

(Eschbach et al., 2021)

mushroom body output neuron 15

Comment: when combined with P{R24H08-p65.AD} (combination referred to as 'MB026B')

when combined with P{R71D08-p65.AD} (combination referred to as 'MB052B')

when combined with P{R65B09-p65.AD} (combination referred to as 'MB050B')

(Ichinose et al., 2021, Scaplen et al., 2021, Aso et al., 2014, Aso et al., 2014)

mushroom body output neuron 18

Comment: when combined with P{R65B09-p65.AD} (combination referred to as 'MB050B')

when combined with P{R71D08-p65.AD} (combination referred to as 'MB052B')

(Ichinose et al., 2021, Scaplen et al., 2021, Aso et al., 2014)

mushroom body output neuron 19

Comment: when combined with P{R71D08-p65.AD} (combination referred to as 'MB052B')

(Ichinose et al., 2021, Aso et al., 2014)

mushroom body output neuron 17

Comment: when combined with P{R71D08-p65.AD} (combination referred to as 'MB052B')

(Ichinose et al., 2021, Aso et al., 2014)

mushroom body output neuron 16

Comment: when combined with P{R24H08-p65.AD} (combination referred to as 'MB026B')

when combined with P{R71D08-p65.AD} (combination referred to as 'MB052B')

(Ichinose et al., 2021, Scaplen et al., 2021, Aso et al., 2014)

mushroom body output neuron 16 | faint

Comment: when combined with P{R24H08-p65.AD} (combination referred to as 'MB026B')

(Aso et al., 2014)

mushroom body output neuron 18 | intense

Comment: when combined with P{R65B09-p65.AD} (combination referred to as 'MB050B')

when combined with P{R71D08-p65.AD} (combination referred to as 'MB052B')

(Aso et al., 2014)

mushroom body output neuron 19 | faint

Comment: when combined with P{R71D08-p65.AD} (combination referred to as 'MB052B')

(Aso et al., 2014)

mushroom body output neuron 17 | faint

Comment: when combined with P{R71D08-p65.AD} (combination referred to as 'MB052B')

(Aso et al., 2014)

mushroom body vertical lobe arborizing neuron 2 alpha' | faint

Comment: when combined with P{R71D08-p65.AD} (combination referred to as 'MB052B')

(Aso et al., 2014)

adult brain | faint | restricted

Comment: when combined with P{R20A02-p65.AD} (combination referred to as 'SS02707')

when combined with P{R50H07-p65.AD} (combination referred to as 'SS02705')

when combined with P{R56H10-p65.AD} (combination referred to as 'SS02712')

when combined with P{R67A06-p65.AD} (combination referred to as 'SS02702')

(Robie et al., 2017)

adult brain | restricted

Comment: when combined with P{R20A02-p65.AD} (combination referred to as 'SS02707')

when combined with P{R50H07-p65.AD} (combination referred to as 'SS02705')

when combined with P{R67A06-p65.AD} (combination referred to as 'SS02702')

(Robie et al., 2017)

adult optic lobe neuron | subset

Comment: when combined with P{R20A02-p65.AD} (combination referred to as 'SS02707')

when combined with P{R56H10-p65.AD} (combination referred to as 'SS02712')

(Robie et al., 2017)

adult ellipsoid body ring neuron R1

Comment: when combined with P{R20A02-p65.AD} (combination referred to as 'SS02707')

when combined with P{R50H07-p65.AD} (combination referred to as 'SS02705')

when combined with P{R67A06-p65.AD} (combination referred to as 'SS02702')

(Robie et al., 2017)

adult ellipsoid body ring neuron R3

Comment: when combined with P{R20A02-p65.AD} (combination referred to as 'SS02707')

when combined with P{R50H07-p65.AD} (combination referred to as 'SS02705')

when combined with P{R56H10-p65.AD} (combination referred to as 'SS02712')

when combined with P{R67A06-p65.AD} (combination referred to as 'SS02702')

(Robie et al., 2017)

adult ellipsoid body ring neuron R4

Comment: when combined with P{R20A02-p65.AD} (combination referred to as 'SS02707')

when combined with P{R50H07-p65.AD} (combination referred to as 'SS02705')

when combined with P{R56H10-p65.AD} (combination referred to as 'SS02712')

when combined with P{R67A06-p65.AD} (combination referred to as 'SS02702')

(Robie et al., 2017)

adult ellipsoid body ring neuron | subset

Comment: when combined with P{R67A06-p65.AD} (combination referred to as 'SS02702')

(Tison et al., 2020)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Images

Virtual Fly Brain - A database to explore neuroanatomy, connectivity and gene expression

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (13)

Stocks containing insertion(s) of this recombinant construct

75978

w¹¹¹⁸; P{R67A06-p65.AD}attP40; P{R11F03-GAL4.DBD}attP2

75981

w¹¹¹⁸; P{R50H07-p65.AD}attP40; P{R11F03-GAL4.DBD}attP2

75983

w¹¹¹⁸; P{R20A02-p65.AD}attP40; P{R11F03-GAL4.DBD}attP2

75988

w¹¹¹⁸; P{R56H10-p65.AD}attP40; P{R11F03-GAL4.DBD}attP2

602538

w¹¹¹⁸; P{R52B07-p65.AD}attP40; P{R11F03-GAL4.DBD}attP2

603381

w¹¹¹⁸; P{R23B09-p65.AD}attP40; P{R11F03-GAL4.DBD}attP2

604524

w¹¹¹⁸; P{VT020956-p65.AD}attP40; P{R11F03-GAL4.DBD}attP2

68365

w¹¹¹⁸; P{R65B09-p65.AD}attP40/CyO; P{R11F03-GAL4.DBD}attP2

68300

w¹¹¹⁸; P{R24H08-p65.AD}attP40; P{R11F03-GAL4.DBD}attP2/TM6B, Tb¹

603027

w¹¹¹⁸; P{R24H08-p65.AD}attP40/CyO; P{R11F03-GAL4.DBD}attP2/TM6B, Tb¹

603001

w¹¹¹⁸; P{R80A11-p65.AD}attP40/CyO, P{2xTb¹-RFP}CyO; P{R11F03-GAL4.DBD}attP2

11869

w¹¹¹⁸; P{R11F03-GAL4.DBD}attP2

More stocks available...

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

11F03-DBD

(Duistermars et al., 2018)

P{R11F03-GAL4.DBD}

(FlyBase, 1992-)

R11F03

(Jovanic et al., 2019, Robie et al., 2017, Aso et al., 2014)

R11F03-ZpGdbd

(Tison et al., 2020)

Secondary FlyBase IDs

References (23)

Report Sections