FB2024_03 , released June 25, 2024
Recombinant Construct: P{R76F05-p65.AD}
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General Information
Symbol
P{R76F05-p65.AD}
FlyBase ID
FBtp0099561
Feature type
transgenic_transposable_element
Component Allele(s)
Hsap\RELAAD.R76F05
Also Known As
R76F05, R76F05-p65ADZp
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Hsap\RELAAD.R76F05
Transgenic product class
Component
Use(s)
Regulatory region(s)
GMR76F05
Encoded product / tool
p65(AD)::Zip+
Description

The R76F05 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
larval mushroom body dopaminergic neuron h1

Comment: when combined with P{R95H02-GAL4.DBD} (combination referred to as 'SS01696')

(Truman et al., 2023, Saumweber et al., 2018)
larval mushroom body dopaminergic neuron f1

Comment: when combined with P{R15B01-GAL4.DBD} (combination referred to as 'MB054B')

when combined with P{VT002847-GAL4.DBD} (combination referred to as 'SS02180')

(Eschbach et al., 2020)
larval mushroom body dopaminergic neuron g1

Comment: when combined with P{R15B01-GAL4.DBD} (combination referred to as 'MB054B')

(Eschbach et al., 2020)
adult stage
dopaminergic PAM neuron 2 | intense

Comment: when combined with P{R23C12-GAL4.DBD} (combination referred to as 'MB109B')

(Yamagata et al., 2015, Aso et al., 2014)
dopaminergic PAM neuron 4 | faint

Comment: when combined with P{R23C12-GAL4.DBD} (combination referred to as 'MB109B')

(Yamagata et al., 2015)
dopaminergic PAM neuron 6 | faint

Comment: when combined with P{R23C12-GAL4.DBD} (combination referred to as 'MB109B')

(Yamagata et al., 2015)
dopaminergic PAM neuron 3 | faint

Comment: when combined with P{R23C12-GAL4.DBD} (combination referred to as 'MB109B')

(Yamagata et al., 2015)
dopaminergic PAM neuron 10

Comment: when combined with P{R32G08-GAL4.DBD} (combination referred to as 'MB213B')

(Yamagata et al., 2015, Aso et al., 2014)
dopaminergic PAM neuron 5 | intense

Comment: when combined with P{R80G12-GAL4.DBD} (combination referred to as 'MB056B')

(Yamagata et al., 2015)
dopaminergic PAM neuron 6 | intense

Comment: when combined with P{R80G12-GAL4.DBD} (combination referred to as 'MB056B')

(Yamagata et al., 2015, Aso et al., 2014)
dopaminergic PAM neuron 2

Comment: when combined with P{R23C12-GAL4.DBD} (combination referred to as 'MB109B')

(Aso and Rubin, 2016, Lewis et al., 2015)
dopaminergic PAM neuron 5

Comment: when combined with P{R80G12-GAL4.DBD} (combination referred to as 'MB056B')

(Lewis et al., 2015, Aso et al., 2014)
dopaminergic PAM neuron 6

Comment: when combined with P{R80G12-GAL4.DBD} (combination referred to as 'MB056B')

(Lewis et al., 2015)
dopaminergic PAM neuron 1 | faint

Comment: when combined with P{R23C12-GAL4.DBD} (combination referred to as 'MB109B')

(Aso et al., 2014)
dopaminergic PAM neuron 11 | faint

Comment: when combined with P{R32G08-GAL4.DBD} (combination referred to as 'MB213B')

(Aso et al., 2014)
dopaminergic PAM neuron 4

Comment: when combined with P{R32G08-GAL4.DBD} (combination referred to as 'MB213B')

(Aso et al., 2014)
mushroom body beta lobe slice 1

Comment: when combined with P{R32G08-GAL4.DBD} (combination referred to as 'MB213B')

(Aso and Rubin, 2016)
mushroom body beta lobe slice 2

Comment: when combined with P{R32G08-GAL4.DBD} (combination referred to as 'MB213B')

(Aso and Rubin, 2016)
dopaminergic PAL neuron | subset

Comment: when combined with P{Ddc-GAL4.DBD}

when combined with P{ple-GAL4.DBD}

(Cichewicz et al., 2017)
dopaminergic PAM neuron | subset

Comment: when combined with P{Ddc-GAL4.DBD}

when combined with P{VT043075-GAL4.DBD} (combination referred to as 'SS35259')

when combined with P{ple-GAL4.DBD}

(Sterne et al., 2021, Cichewicz et al., 2017)
dopaminergic PPL1 neuron | subset

Comment: when combined with P{Ddc-GAL4.DBD}

when combined with P{ple-GAL4.DBD}

(Cichewicz et al., 2017)
dopaminergic PPM1 neuron | subset

Comment: when combined with P{Ddc-GAL4.DBD}

when combined with P{ple-GAL4.DBD}

(Cichewicz et al., 2017)
dopaminergic PPM2 neuron | subset

Comment: when combined with P{Ddc-GAL4.DBD}

when combined with P{ple-GAL4.DBD}

(Cichewicz et al., 2017)
dopaminergic PPM3 neuron | subset

Comment: when combined with P{Ddc-GAL4.DBD}

when combined with P{ple-GAL4.DBD}

(Cichewicz et al., 2017)
dopaminergic PPL2ab neuron | subset

Comment: when combined with P{ple-GAL4.DBD}

when combined with P{Ddc-GAL4.DBD}

(Cichewicz et al., 2017)
AOTU neuron of the dopaminergic PAL cluster

Comment: when combined with P{DAT-B-GAL4.DBD}

when combined with P{R61H03-GAL4.DBD}

(Xie et al., 2018)
SMP-gamma neuron of the dopaminergic PPL1 cluster

Comment: when combined with P{DAT-B-GAL4.DBD}

when combined with P{ple-GAL4.DBD.TH-D}

(Xie et al., 2018)
VMNP-LO neuron of the dopaminergic PAL cluster

Comment: when combined with P{DAT-B-GAL4.DBD}

when combined with P{R61H03-GAL4.DBD}

when combined with P{ple-GAL4.DBD.TH-C}

(Xie et al., 2018)
dFB neuron of the dopaminergic PPL1 cluster

Comment: when combined with P{DAT-B-GAL4.DBD}

when combined with P{R60F07-GAL4.DBD}

(Xie et al., 2018)
mushroom body pedunculus-medial lobe arborizing neuron 1

Comment: when combined with P{DAT-B-GAL4.DBD}

when combined with P{R60F07-GAL4.DBD}

when combined with P{ple-GAL4.DBD.TH-F}

(Xie et al., 2018)
SMP-PED neuron of the dopaminergic PPL1 cluster

Comment: when combined with P{R60F07-GAL4.DBD}

(Xie et al., 2018)
WED neuron of the dopaminergic PPM2 cluster

Comment: when combined with P{R60F07-GAL4.DBD}

when combined with P{R61H03-GAL4.DBD}

when combined with P{ple-GAL4.DBD.TH-C}

when combined with P{ple-GAL4.DBD.TH-D}

when combined with P{ple-GAL4.DBD.TH-F}

(Xie et al., 2018)
LAL-SEG neuron of the dopaminergic PPM2 cluster

Comment: when combined with P{R61H03-GAL4.DBD}

when combined with P{ple-GAL4.DBD.TH-C}

when combined with P{ple-GAL4.DBD.TH-D}

when combined with P{ple-GAL4.DBD.TH-F}

(Xie et al., 2018)
VLP neuron of the dopaminergic PPM2 cluster

Comment: when combined with P{R61H03-GAL4.DBD}

when combined with P{ple-GAL4.DBD.TH-D}

(Xie et al., 2018)
mushroom body medial-vertical lobe arborizing neuron 1

Comment: when combined with P{R61H03-GAL4.DBD}

(Xie et al., 2018)
VLP-AMMC neuron of the dopaminergic PPM2 cluster

Comment: when combined with P{ple-GAL4.DBD.TH-C}

when combined with P{ple-GAL4.DBD.TH-F}

(Xie et al., 2018)
adult dopaminergic AUM neuron of the gnathal ganglion

Comment: when combined with P{ple-GAL4.DBD.TH-F}

(Xie et al., 2018)
LH-CA neuron of the dopaminergic PPL2ab cluster

Comment: when combined with P{ple-GAL4.DBD.TH-F}

(Xie et al., 2018)
SMP-PRW neuron of the dopaminergic PAL cluster

Comment: when combined with P{ple-GAL4.DBD.TH-F}

(Xie et al., 2018)
VLP-SAD neuron of the dopaminergic PPM2 cluster

Comment: when combined with P{ple-GAL4.DBD.TH-F}

(Xie et al., 2018)
adult ascending neuron | subset

Comment: when combined with P{VT000357-GAL4.DBD} (combination referred to as 'SS35281')

when combined with P{VT043075-GAL4.DBD} (combination referred to as 'SS35259')

(Sterne et al., 2021)
adult asteroid subesophageal neuron

Comment: when combined with P{VT000357-GAL4.DBD} (combination referred to as 'SS35281')

(Sterne et al., 2021)
adult balloon subesophageal neuron

Comment: when combined with P{VT043075-GAL4.DBD} (combination referred to as 'SS35259')

(Sterne et al., 2021)
adult neuron | subset of adult abdominal neuromere

Comment: when combined with P{VT043075-GAL4.DBD} (combination referred to as 'SS35259')

(Sterne et al., 2021)
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Images
Virtual Fly Brain - A database to explore neuroanatomy, connectivity and gene expression
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments

FlyBase curator comment: According to correspondence with the authors, some split combinations listed in the methods of the paper are incorrect; the correct combinations (MB002B=R12C11-AD+R14C08-DBD, MB316B=R58E02-AD+R93G08-DBD, MB056B=R76F05-AD+R80G12-DBD, MB047B=R58E02-AD+R93A09-DBD) are listed on FlyLight so these are the ones that have been associated with the paper.

(Lewis et al., 2015)
Stocks (15)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (32)