FB2024_03 , released June 25, 2024
Recombinant Construct: TI{ywing2+}
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General Information
Symbol
TI{ywing2+}
FlyBase ID
FBtp0129877
Feature type
engineered_region
Component Allele(s)
Size
Expression Data
Associated insertion(s)
13 available
Molecular map
Description and Uses
Description

TI{ywing2+} represents a DNA segment that has been inserted into the genome by homology directed repair using CRISPR/Cas9 in combination with a donor plasmid. The inserted TI{ywing2+} DNA is not flanked by transposable element ends and consists of the ywing2+ dominant marker flanked by the nucleotides 'GG' and 'CC' upstream and downstream, respectively. These flanking dinucleotides generate two novel Cas9 target sites that are not present in the endogenous sequence, allowing for removal of the TI{ywing2+} DNA segment via a second round of homology directed repair.

(Li-Kroeger et al., 2018)
Construct components
Component
Use(s)
Marker(s)
ywing2+
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (9)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
TI{ywing2+}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (6)