FB2024_03 , released June 25, 2024
Recombinant Construct: PBac{Ubi(FRT.stop)GBP6:tkv-2A-GBP1:put}
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General Information
Symbol
PBac{Ubi(FRT.stop)GBP6:tkv-2A-GBP1:put}
FlyBase ID
FBtp0150469
Feature type
transgenic_transposable_element
Component Allele(s)
putFRT.Ubi.VHH(GBP1),Tag:FLAG
tkvFRT.Ubi.VHH(GBP6),Tag:V5
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele tkvFRT.Ubi.VHH(GBP6),Tag:V5
Transgenic product class
cDNA
(Stapornwongkul et al., 2020)
wild_type
(Stapornwongkul et al., 2020)
Component
Use(s)
Encoded product / tool
tkv
Tagged with
Tag:V5
VHH(GBP6)
Also carries
FRT
Description

A Ubiquitin promoter is fused upstream of an FRT stop cassette, followed by a bicistronic transcript that encodes two open reading frames separated by a 2A linker (this results in separate translation of each protein). The first ORF comprises a tkv cDNA (clone LD08886) into which the VHH(GBP6) coding sequence has been inserted (flanked by linker sequences that includes 2xTag:V5 in the upstream linker) in between residues A66 and R67. The second ORF comprises a put cDNA (clone LP03259) into which the VHH(GBP1) coding sequence has been inserted (flanked by linker sequences that includes 3xTag:FLAG in the upstream linker) in between residues G29 and S30.

(Stapornwongkul et al., 2020)
Component allele putFRT.Ubi.VHH(GBP1),Tag:FLAG
Transgenic product class
cDNA
(Stapornwongkul et al., 2020)
wild_type
(Stapornwongkul et al., 2020)
Component
Use(s)
Encoded product / tool
put
Tagged with
Tag:FLAG
VHH(GBP1)
Also carries
FRT
Description

A Ubiquitin promoter is fused upstream of an FRT stop cassette, followed by a bicistronic transcript that encodes two open reading frames separated by a 2A linker (this results in separate translation of each protein). The first ORF comprises a tkv cDNA (clone LD08886) into which the VHH(GBP6) coding sequence has been inserted (flanked by linker sequences that includes 2xTag:V5 in the upstream linker) in between residues A66 and R67. The second ORF comprises a put cDNA (clone LP03259) into which the VHH(GBP1) coding sequence has been inserted (flanked by linker sequences that includes 3xTag:FLAG in the upstream linker) in between residues G29 and S30.

(Stapornwongkul et al., 2020)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
PBac{Ubi(FRT.stop)GBP6:tkv-2A-GBP1:put}
(FlyBase, 1996-)
SR
(Stapornwongkul et al., 2020)
ubi-[>STOP>Nb2highTkv 2A Nb1highPut]
(Stapornwongkul et al., 2020)
ubi-[FRT STOP FRT GBP6:Tkv PT2A GBP1:Put]
(Stapornwongkul et al., 2020)
Secondary FlyBase IDs
    References (2)