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General Information
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| Term |
western blot |
ID (Ontology) |
MI:0113 (Molecular Interactions) |
| Definition |
Western blot is a procedure to identify and quantify proteins. A mixture of protein is first submitted to an electrophoresis in denaturing condition and then electro-transferred from the gel to a membrane. The membrane is then incubated with a primary antibody specific for a given protein or a specific residue modification in the sample under analysis. A secondary antibody, radiolabelled or fused to fluorophore or to a chromogenic enzyme, targets the first antibody and allows the visualisation of the protein band on the membrane.[ PubMed:14755292 ] |
| Also Known As |
"Immuno blot" |
| Comment |
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Links to Extrernal Ontologies
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Annotations
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Records annotated with this term OR any of its CHILD TERMS
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Records annotated with this exact term (annotations to child terms are NOT included)
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| Data Class | Field | Records |
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| Insertions (FBti) | EXPRESSION_STAGE_TISSUE_POSITION | 10 |
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Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records
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| Full annotation statements | Relevant FlyBase reports |
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| Genes | Interaction Groups | Insertions | Constructs |
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western blot (all annotations which use CV term, excluding "NOT" statements) | 457 | 4654 | 10 | 3 |
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Relationships