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General Information
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| Term |
rna three hybrid |
ID (Ontology) |
MI:0438 (Molecular Interactions) |
| Definition |
In vivo reconstruction of specific RNA-proteins interactions. The DNA binding and transcription activator domains of GAL4 are brought together via the interaction of recombinant RNA. The first hybrid protein contains the DNA binding domain of GAL4 fused to RevM10 (a mutated RNA binding protein of HIV-1 that binds specifically to the Rev responsive element RRE of the env gene). A recombinant RNA contains the RRE sequence and a target RNA sequence X. The second hybrid protein contains the activation domain of GAL4 fused to protein Y tested for its ability to bind the target RNA X. If this interaction occurs the three hybrid reconstructs GAL4 and the transcription of a reporter gene is activated.[ PubMed:12162957 PubMed:8972875 ] |
| Also Known As |
"rna 3-hybrid" ; "rna tri hybrid" ; "rna-three hybrid" (for all, see Synonyms field below) |
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Links to Extrernal Ontologies
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Annotations
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Records annotated with this term OR any of its CHILD TERMS
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Records annotated with this exact term (annotations to child terms are NOT included)
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No relevant records available
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Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records
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| Full annotation statements | Relevant FlyBase reports |
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| Interaction Groups |
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rna three hybrid (all annotations which use CV term, excluding "NOT" statements) | 21 |
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Relationships