[54B16-54B16];[54C1-54C1];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
54B16;54C1
A 15,396 nt deletion corresponding to deficiency FDD-0000787 in www.drosdel.org.uk.
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
Inferred to overlap with: Df(2R)BSC161.
The presence of P+PBac{XP5.RB3}BSC406 was verified using the PCR methods and primers described in FBrf0175003 with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods.
The cytological breakpoints of Df(2R)BSC406 predicted from the Release 5 genomic coordinates of the progenitor P{XP}CG14478d09297 and PBac{RB}CG11419e01070 transposable element insertions sites are 54B16;54C1.