[70A5-70A5];[70C5-70C5];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
70A5;70C5
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
1: P{XP}d06352
2: PBac{RB}Rgle01319
Inferred to overlap with: Df(3L)Exel6119.
Inferred to overlap with: Df(3L)Exel9017.
The presence of P+PBac{XP5.RB3}BSC614 was verified using the PCR methods and primers described in FBrf0175003, with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods.
The cytological breakpoints of
Df(3L)BSC614 predicted from the Release 5 genomic coordinates of the insertion sites of the progenitor P{XP}d06352 and PBac{RB}Rgle01319 transposable elements are 70A5;70C5.