[84B2-84B2];[84C3-84C3];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
84B2;84C3
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
Df(3R)BSC633/TM6C, Sb1 cu1 males are sterile.
The presence of P+PBac{XP5.RB3}BSC633 was verified using the PCR methods and primers described in FBrf0175003, with the substitution of the primer 5'-GCTTCTAAACGCTTACGCATAAACGATG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods.
The cytological breakpoints of Df(3R)BSC633 predicted from the Release 5 genomic coordinates of the insertions sites of the progenitor transposable elements P{XP}Alyd01000 and PBac{RB}e03258 are 84B2;84C3.