The P{FRT(Target70,let-7-C^KO1)} has been used as a donor construct for end-in homologous recombination of the genomic region containing mir-100, let-7 and mir-125, which should result in partial duplication of the region, and an Scer\FRT site from the donor construct becoming inserted at this region of the genome in the targeted chromosome. This has been carried out in flies carrying PBac{WH}CG10283^f03195, so that if this partial duplication is created, recombination can occur between the Scer\FRT site in the targeted chromosome and the Scer\FRT site in the insertion, resulting in a deletion of the sequence between the two sites. The end result is a deletion of approximately 1.1kb that removes the sequences encoding the mature mir-100, let-7 and mir-125 microRNAs, plus the CG10283 gene is also disrupted as a result of the recombination events.

1071bp deletion that removes the mir-100, let-7 and mir-125 microRNAs. The CG10283 gene is also disrupted.

Df(2L)let-7-C^KO1/Df(2L)BSC149 animals show partial lethality.

Df(2L)let-7-C^KO1/Df(2L)BSC149 males and females show a significant decrease in median lifespan compared to controls.

Df(2L)let-7-C^KO1/Df(2L)BSC149 males are sterile. Df(2L)let-7-C^KO1/Df(2L)BSC149 females are fertile.

The musculature and myoblasts of Df(2L)let-7-C^GKI/Df(2L)let-7-C^KO1 larvae appear normal.

43% of Df(2L)let-7-C^KO1/Df(2L)let-7-C^GKI animals die prematurely during the course of development, with 74% of these arresting at the very end of metamorphosis. The remaining 57% of Df(2L)let-7-C^KO1/Df(2L)let-7-C^GKI animals eclose as adults, but have chronic defects in function, including severely reduced motility, climbing ability, flight and male and female fertility.

The adult neuromusculature of Df(2L)let-7-C^KO1/Df(2L)let-7-C^GKI animals displays persistent pupal as well as immature adult characteristics. The dorsal internal oblique muscles (DIOMs) that ordinarily decay during post-eclosion maturation fail to disappear in older mutant adults in most cases, and the neuromuscular junctions connecting DIOMs and their innervating motoneurons also fail to decay. The dorsal muscles (DMs) of mutant adults are smaller than those of age-matched controls. The mutant DMs are narrower and contain fewer nuclei compared to wild-type controls. The neuromuscular junctions of the Dms are either completely absent, shorter in length than normal, or devoid of boutons, appearing as long, thin processes along the length of the DM.

Homozygotes show 100% lethality.

Mutant pupae and adults appear morphologically normal.

Associated with: CG10283^K01.

The "let-7-C[KO1]" chromosome carries a deletion of approximately 1.1kb that removes the sequences encoding the mature mir-100, let-7 and mir-125 microRNAs. The CG10283 gene is also disrupted in this chromosome.

The "let-7-C[KO1]" chromosome carries a 1071bp deletion that removes the mir-100, let-7 and mir-125 microRNAs. The CG10283 gene is also disrupted on this chromosome.