FB2024_03 , released June 25, 2024
Allele: Dmel\adp60
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General Information
Symbol
Dmel\adp60
Species
D. melanogaster
Name
FlyBase ID
FBal0000386
Feature type
allele
Associated gene
Dmel\adp
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
spontaneous
(Doane, 1999.3.16, Doane, 1963)
natural population
(Doane, 1996)
Progenitor genotype
Cytology
Description

23bp deletion, resulting in a frameshift that produces two changed amino acid residues followed by a premature stop codon.

(Hader et al., 2003)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
delins
2R:18,153,110..18,153,134 [+]
Comment:

Reported as a 23bp deletion which cause a frameshift and early termination. The published sequence show that a 25bp region is missing and TA is inserted in the gap, resulting in a frameshift and early termination.

Inserted_sequence:

TA

(Hader et al., 2003)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  obesity
      CEA
      (Suh et al., 2007, Hader et al., 2003)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous adp60 mutants have decreased mobility compared with heterozygotes.

      Homo- and heterozygous adp60 mutant flies deprived of food survive longer than wild-type controls under the same conditions. Survival is adp60 > adp60/+ > +/+. Triglyceride content of the flies follows the same trend.

      (Suh et al., 2007)

      After 1 week of ad libitum feeding, the fat body cells of adp60 flies contain greatly enlarged lipid droplets compared to controls. In addition, the mutant animals show a twofold higher accumulation of triglycerides, and a variable, slight reduction of glycogen levels to 70-80% of normal levels.

      adp60 adults show increased resistance to starvation compared to control flies.

      (Hader et al., 2003)

      Homozygous females are heavier than control females. Heterozygous females are not significantly different in weight from control females.

      (Wisotzkey et al., 2003)

      Adults are obese.

      (Doane, 1996)

      Corpus allatum hypertrophies in mated females. Females are fertile but egg hatchability is reduced. Adult emergence is low.

      (Doane, 1963)

      Adult fat body hypertrophies as cells become distorted by enormous oil globules. Lipid accumulated at expense of glycogen in fat body; yolk deposition retarded (FBrf0065652; FBrf0034729). Lipid content and fatty acid profiles compared for various developmental stages in adp60 and wild type (FBrf0044927). Abnormal fat bodies visible through body wall of 6-day-old and older adults when submerged in 95% alcohol and then water. Corpus allatum of mated females hypertrophies. Females fertile but egg hatchability reduced to 45-90%, depending on residual genome; dorsal appendages of chorion convoluted or fused (FBrf0015801); adult emergence lowered to 33-85%. (FBrf0015272).Heterozygotes show desiccation tolerance superior to that of wild type or adp60 homozygotes (FBrf0039608). RK3.

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      adp60/Df(2L)C28 double heterozygous females are significantly heavier (approximately 21% heavier) than control females.

      adp60/Df(2L)C28 double heterozygotes eclose at the same rate as adp60/+ flies.

      The weight of adp60/tocP and adp60/Mad12 double heterozygous flies is not significantly different from that of control flies.

      adp60/Df(2L)C28 double heterozygous females expressing Hydr2Scer\UAS.cWa under the control of Scer\GAL4hs.PB (using heat shock) show no significant difference in weight compared to control females.

      (Wisotzkey et al., 2003)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      adp60 is rescued by adp+t3.6

      (Hader et al., 2003)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Availability

      Stated to be lost.

      (Losurdo et al., 2024)
      Notes on Origin
      Discoverer

      Doane, 1960.

      Arose in: Kaduna wild population maintained at Edinburgh. Identified in 1960.

      (Doane, 1999.3.16)

      Isolated from: Kaduna, Nigeria population.

      (Doane, 1996, Doane, 1963)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      adp60
      (Losurdo et al., 2024, Suh et al., 2007, Beller et al., 2006, Hader et al., 2003, Wisotzkey et al., 2003)
      fs(2)adp
      Name Synonyms
      Secondary FlyBase IDs
        References (16)