FlyBase Allele Report: Dmel\ash1[22]

ash1²¹/ash1²² transheterozygotes show a homeotic phenotype, with haltere towards wing and third leg towards second leg transformations, as well as partial transformation of abdominal segments 6 and 5 towards segments 5 and 4, which is visible from the partial loss of pigmentation on tergites 5 and 6 and appearance of bristles on sternite 6.

ash1²¹/Df(3L)Exel9011 are near fully lethal at early pupal stage, before the adult cuticle is formed. The single male with the adult cuticle developed enough for examination shows clear posterior to anterior transformations of the third thoracic and abdominal segments.

(Dorafshan et al., 2019)

ash1²¹/ash1²² adults or ash1²²/Df(3L)Exel9011 individuals rescued to adulthood by two copies of either ash1^{ΔBAH.Ubi-p63E}, ash1^{ΔPHD.Ubi-p63E} or ash1^{ΔSET.Ubi-p63E} show homeotic transformation: transformation of the third thoracic segment (T3) to second thoracic segment (T2), including partial transformation of haltere to wing, characterized by the appearance of bristles on the haltere, appearance of hypopleural bristles on T3, and the third leg to second leg transformation manifested by the appearance of apical and pre-apical bristles on the third leg; transformation of posterior abdominal segments to more anterior fate, visible by the partial loss of pigmentation in tergites 5.

(Dorafshan et al., 2019)

ash1²²/ash1²² animals that lack zygotically expressed Ash1 protein and either contain or lack maternally deposited Ash1 protein (due to the germline clones in the mother) develop to pupal stage and die as pharate adults.

ash1²²/ash1²² pharate adults that lack both maternally deposited and zygotically expressed Ash1 protein exhibit a spectrum of anteriorly directed homeotic transformations (HOX loss-of-function syndrome) without any other morphological defects compared to controls. Specifically, they exhibit transformation of the third to the second thoracic segment; transformations of the abdominal segments A5 and A6 towards A4 (most noticeable by the loss of pigmentation in males); and development of an A7 segment that is normally suppressed in controls.

(Schmähling et al., 2018)

Most ash1^R1288A/ash1²² transheterozygotes exhibit a third leg to second leg partial transformation, as indicated by the presence of ectopic apical bristles, and exhibit a haltere to wing partial transformation, as indicated by the presence of ectopic bristles on the pedicel, as compared to wild-type and heterozygous controls.

(Huang et al., 2017)

Homozygous haltere discs are larger than normal, probably due to a partial transformation into wing discs.

(Papp and Muller, 2006)

Lethality occurs during the larval and pupal stages. Double heterozygotes with Ubx^abx-1, trx^el2 or brm² exhibit transformation of third leg to second leg identity and haltere to wing transformation.

(Tripoulas et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ash1^R1288A/ash1²² has visible phenotype, suppressible by Df(2R)XL26/+

(Huang et al., 2017)

ash1^R1288A/ash1²² has homeotic phenotype, suppressible by Df(2R)XL26/+

(Huang et al., 2017)

ash1^R1288A/ash1²² has visible phenotype, suppressible | partially by Scer\GAL4^arm.PS/Caf1-55::MRG15^UAS.cHa

(Huang et al., 2017)

ash1^R1288A/ash1²² has homeotic phenotype, suppressible | partially by Scer\GAL4^arm.PS/Caf1-55::MRG15^UAS.cHa

(Huang et al., 2017)

ash1^R1288A/ash1²² has visible phenotype, suppressible | partially by Scer\GAL4^arm.PS/Caf1-55::MRG15^Δchromo.UAS

(Huang et al., 2017)

ash1^R1288A/ash1²² has homeotic phenotype, suppressible | partially by Scer\GAL4^arm.PS/Caf1-55::MRG15^Δchromo.UAS

(Huang et al., 2017)

ash1^R1288A/ash1²² has visible phenotype, suppressible | partially by Caf1-55::Hsap\MORF4L1^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

ash1^R1288A/ash1²² has homeotic phenotype, suppressible | partially by Caf1-55::Hsap\MORF4L1^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

NOT suppressed by

Statement

Reference

ash1²²/ash1²¹ has homeotic | adult stage phenotype, non-suppressible by NSD^ds46/NSD^ds46

(Dorafshan et al., 2019)

ash1²²/ash1²¹ has visible | adult stage phenotype, non-suppressible by NSD^ds46/NSD^ds46

(Dorafshan et al., 2019)

ash1^R1288A/ash1²² has homeotic phenotype, non-suppressible by Caf1-55^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

ash1^R1288A/ash1²² has visible phenotype, non-suppressible | partially by Scer\GAL4^arm.PS/Caf1-55::MRG15^UAS.cHa

(Huang et al., 2017)

ash1^R1288A/ash1²² has homeotic phenotype, non-suppressible | partially by Scer\GAL4^arm.PS/Caf1-55::MRG15^UAS.cHa

(Huang et al., 2017)

ash1^R1288A/ash1²² has visible phenotype, non-suppressible by MRG15^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

ash1^R1288A/ash1²² has homeotic phenotype, non-suppressible by MRG15^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

ash1^R1288A/ash1²² has visible phenotype, non-suppressible by Caf1-55^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

Enhancer of

Statement

Reference

ash1[+]/ash1²² is an enhancer of visible | homeotic phenotype of Dsp1¹

(Decoville et al., 2001)

Suppressor of

Statement

Reference

ash1[+]/ash1²² is a suppressor of visible | dominant phenotype of gcm^Pyx

(Popkova et al., 2012)

Other

Statement

Reference

Df(2L)His^C, Df(3L)Exel9011/ash1²², His3^HisTA.H3K36R has lethal - all die before end of larval stage phenotype

(Dorafshan et al., 2019)

Df(2L)His^C, Df(3L)Exel9011/ash1²², His3^HisTA.H3K36R has lethal - all die before end of first instar larval stage phenotype

(Dorafshan et al., 2019)

Df(2L)His^C, His3^HisTA.H3K36R, ash1²² has some die during P-stage phenotype

(Dorafshan et al., 2019)

Df(2L)His^C, His3^HisTA.H3K36R, ash1²² has lethal - all die before end of P-stage phenotype

(Dorafshan et al., 2019)

Df(2L)His^C, His3^HisTA.H3K36R, ash1²² has majority die during larval stage phenotype

(Dorafshan et al., 2019)

Df(2L)His^C, Df(3L)Exel9011/ash1²², His3^HisTA.H3K36R has lethal - all die before end of P-stage phenotype

(Dorafshan et al., 2019)

ash1²², sktl[+]/sktl^Δ20 has visible | dominant | homeotic phenotype

(Cheng and Shearn, 2004)

ash1²², sktl[+]/sktl^Δ15 has visible | dominant | homeotic phenotype

(Cheng and Shearn, 2004)

ash1²², sktl^Δ15 has visible | dominant | homeotic phenotype

(Cheng and Shearn, 2004)

ash1²², sktl^Δ20 has visible | dominant | homeotic phenotype

(Cheng and Shearn, 2004)

Asx¹³, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Psc¹, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Scm^D1, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Scm^M56, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Su(z)2¹, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Su(z)6¹, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Asx³, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Su(z)7¹, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Trl^R85, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Asx[+]/Asx¹³, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Asx³/Asx[+], ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Asx^XF23/Asx[+], ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Df(2L)E110/+, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Df(2L)Gpdh1-A/+, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

+/Df(2L)Mdh, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Df(2L)cl-h1/+, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Df(2L)cl-h3/+, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

E(Pc)¹, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Df(2L)cl-h4/+, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Df(2R)Pu-D17/+, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Df(2R)or-BR6/+, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Df(3L)Ar14-8/+, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Df(3L)vin6/+, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

E(Pc)[+]/E(Pc)², ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

E(z)[+]/E(z)⁵, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Psc[+]/Psc¹, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Sce[+]/Sce^D1, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Scm^D1/Scm[+], ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

E(Pc)², ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Scm^M56/Scm[+], ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Su(z)2¹/Su(z)2[+], ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Su(z)6[+]/Su(z)6¹, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Su(z)7[+]/Su(z)7¹, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Trl[+]/Trl^R85, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

lid[+]/Kdm5¹⁰⁴²⁴, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Kdm5^k06801/lid[+], ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

E(Pc)[+]/E(Pc)¹, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

E(z)⁵, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Kdm5¹⁰⁴²⁴, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Kdm5^k06801, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Sce^D1, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Asx^XF23, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Phenotype Manifest In

Suppressed by

Statement

Reference

ash1^R1288A/ash1²² has haltere pedicel | adult stage phenotype, suppressible by Df(2R)XL26/+

(Huang et al., 2017)

ash1^R1288A/ash1²² has adult sensillum phenotype, suppressible by Df(2R)XL26/+

(Huang et al., 2017)

ash1^R1288A/ash1²² has haltere pedicel | adult stage phenotype, suppressible | partially by Scer\GAL4^arm.PS/Caf1-55::MRG15^UAS.cHa

(Huang et al., 2017)

ash1^R1288A/ash1²² has adult sensillum phenotype, suppressible | partially by Scer\GAL4^arm.PS/Caf1-55::MRG15^UAS.cHa

(Huang et al., 2017)

ash1^R1288A/ash1²² has haltere pedicel | adult stage phenotype, suppressible | partially by Scer\GAL4^arm.PS/Caf1-55::MRG15^Δchromo.UAS

(Huang et al., 2017)

ash1^R1288A/ash1²² has adult sensillum phenotype, suppressible | partially by Scer\GAL4^arm.PS/Caf1-55::MRG15^Δchromo.UAS

(Huang et al., 2017)

ash1^R1288A/ash1²² has haltere pedicel | adult stage phenotype, suppressible | partially by Caf1-55::Hsap\MORF4L1^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

ash1^R1288A/ash1²² has adult sensillum phenotype, suppressible | partially by Caf1-55::Hsap\MORF4L1^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

NOT suppressed by

Statement

Reference

ash1²²/ash1²¹ has haltere phenotype, non-suppressible by NSD^ds46/NSD^ds46

(Dorafshan et al., 2019)

ash1²²/ash1²¹ has metathoracic leg phenotype, non-suppressible by NSD^ds46/NSD^ds46

(Dorafshan et al., 2019)

ash1²²/ash1²¹ has wing | ectopic phenotype, non-suppressible by NSD^ds46/NSD^ds46

(Dorafshan et al., 2019)

ash1²²/ash1²¹ has mesothoracic leg | ectopic phenotype, non-suppressible by NSD^ds46/NSD^ds46

(Dorafshan et al., 2019)

ash1²²/ash1²¹ has adult abdominal segment 5 phenotype, non-suppressible by NSD^ds46/NSD^ds46

(Dorafshan et al., 2019)

ash1²²/ash1²¹ has adult abdominal segment 6 phenotype, non-suppressible by NSD^ds46/NSD^ds46

(Dorafshan et al., 2019)

ash1²²/ash1²¹ has adult abdominal segment 4 phenotype, non-suppressible by NSD^ds46/NSD^ds46

(Dorafshan et al., 2019)

ash1^R1288A/ash1²² has haltere pedicel | adult stage phenotype, non-suppressible by MRG15^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

ash1^R1288A/ash1²² has adult sensillum phenotype, non-suppressible by MRG15^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

ash1^R1288A/ash1²² has haltere pedicel | adult stage phenotype, non-suppressible by Caf1-55^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

ash1^R1288A/ash1²² has adult sensillum phenotype, non-suppressible by Caf1-55^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

ash1^R1288A/ash1²² has metathoracic leg | adult stage phenotype, non-suppressible by Scer\GAL4^arm.PS/Caf1-55::MRG15^UAS.cHa

(Huang et al., 2017)

ash1^R1288A/ash1²² has leg sensillum | ectopic | adult stage phenotype, non-suppressible by Scer\GAL4^arm.PS/Caf1-55::MRG15^UAS.cHa

(Huang et al., 2017)

Enhancer of

Statement

Reference

ash1[+]/ash1²² is an enhancer of wing vein | ectopic phenotype of Snr1^E1

(Marenda et al., 2004)

ash1²² is an enhancer of eye phenotype of CycE^JP

(Brumby et al., 2002)

ash1[+]/ash1²² is an enhancer of adult abdominal segment 4 | ectopic phenotype of E(z)^Trm

(Bajusz et al., 2001)

ash1[+]/ash1²² is an enhancer of adult abdominal segment 5 phenotype of E(z)^Trm

(Bajusz et al., 2001)

ash1[+]/ash1²² is an enhancer of adult mesothoracic segment | ectopic phenotype of E(z)^Trm

(Bajusz et al., 2001)

ash1[+]/ash1²² is an enhancer of adult metathoracic segment phenotype of E(z)^Trm

(Bajusz et al., 2001)

ash1[+]/ash1²² is an enhancer of adult prothoracic segment phenotype of E(z)^Trm

(Bajusz et al., 2001)

ash1[+]/ash1²² is an enhancer of wing | ectopic phenotype of Dsp1¹

(Decoville et al., 2001)

ash1[+]/ash1²² is an enhancer of haltere phenotype of Dsp1¹

(Decoville et al., 2001)

NOT Enhancer of

Statement

Reference

ash1²² is a non-enhancer of eye phenotype of Scer\GAL4^ey.PH, osa^UAS.cCa

(Collins et al., 1999)

Suppressor of

Statement

Reference

ash1[+]/ash1²² is a suppressor of chaeta | increased number phenotype of gcm^Pyx

(Popkova et al., 2012)

ash1[+]/ash1²² is a suppressor of wing vein | ectopic phenotype of Snr1^E1

(Marenda et al., 2004)

ash1²² is a suppressor of wing phenotype of Scer\GAL4^OK386, nej^UAS.cMa

(Bantignies et al., 2000)

NOT Suppressor of

Statement

Reference

ash1²² is a non-suppressor of eye phenotype of Scer\GAL4^ey.PH, osa^UAS.cCa

(Collins et al., 1999)

Other

Statement

Reference

ash1²², sktl[+]/sktl^Δ20 has mesothoracic leg phenotype

(Cheng and Shearn, 2004)

ash1²², sktl[+]/sktl^Δ20 has metathoracic leg | ectopic phenotype

(Cheng and Shearn, 2004)

ash1²², sktl[+]/sktl^Δ15 has mesothoracic leg phenotype

(Cheng and Shearn, 2004)

ash1²², sktl[+]/sktl^Δ15 has metathoracic leg | ectopic phenotype

(Cheng and Shearn, 2004)

ash1²², sktl^Δ15 has mesothoracic leg phenotype

(Cheng and Shearn, 2004)

ash1²², sktl^Δ15 has metathoracic leg | ectopic phenotype

(Cheng and Shearn, 2004)

ash1²², sktl^Δ20 has mesothoracic leg phenotype

(Cheng and Shearn, 2004)

ash1²², sktl^Δ20 has metathoracic leg | ectopic phenotype

(Cheng and Shearn, 2004)

Asx¹³, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Psc¹, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Scm^D1, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Scm^M56, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Su(z)2¹, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Su(z)6¹, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Su(z)7¹, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Trl^R85, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Asx[+]/Asx¹³, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Asx³/Asx[+], ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Asx^XF23/Asx[+], ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Asx³, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Df(2L)E110/+, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Df(2L)Gpdh1-A/+, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

+/Df(2L)Mdh, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Df(2L)cl-h1/+, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Df(2L)cl-h3/+, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Df(2L)cl-h4/+, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Df(2R)Pu-D17/+, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Df(2R)or-BR6/+, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Df(3L)Ar14-8/+, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Df(3L)vin6/+, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

E(Pc)¹, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

E(Pc)[+]/E(Pc)², ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

E(z)[+]/E(z)⁵, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Psc[+]/Psc¹, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Sce[+]/Sce^D1, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Scm^D1/Scm[+], ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Scm^M56/Scm[+], ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Su(z)2¹/Su(z)2[+], ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Su(z)6[+]/Su(z)6¹, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Su(z)7[+]/Su(z)7¹, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Trl[+]/Trl^R85, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

E(Pc)², ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

lid[+]/Kdm5¹⁰⁴²⁴, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Kdm5^k06801/lid[+], ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

E(Pc)[+]/E(Pc)¹, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Kdm5¹⁰⁴²⁴, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Kdm5^k06801, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

E(z)⁵, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Sce^D1, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Asx^XF23, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The haltere to wing partial transformation exhibited by most ash1^R1288A/ash1²² transheterozygotes is fully suppressed by one copy of Df(2R)XL26 and is partially suppressed by the expression of Caf1-55::MRG15^Scer\UAS.cHa or Caf1-55::MRG15^{Δchromo.Scer\UAS}, but not of MRG15^Scer\UAS.cHa or Caf1-55^Scer\UAS.cHa, under the control of Scer\GAL4^arm.PS; their third leg to second leg partial transformation, however, is not suppressed by the expression of Caf1-55::MRG15^Scer\UAS.cHa under the control of Scer\GAL4^arm.PS.

(Huang et al., 2017)

ash1²² sktl^Δ15 double heterozygotes show a homeotic transformation of third leg to second leg (3.7% penetrance). ash1²² sktl^Δ20 double heterozygotes show a homeotic transformation of third leg to second leg (2.4% penetrance).

(Cheng and Shearn, 2004)

At 29^oC, ash1²² suppresses the ectopic vein that extends distally from the posterior crossvein in Snr1^E1 animals. but enhances the extra vein material seen posterior of the L5 wing vein.

(Marenda et al., 2004)

The frequency of transformation of halteres into wings seen in Dsp1¹/Y flies is enhanced by ash1²²/+. The enhancement is dramatically reduced if the female parents are heterozygous rather than homozygous for Dsp1¹.

(Decoville et al., 2001)

Double heterozygotes with Df(2L)GpdhA, Df(2L)cl-h4, Df(2L)cl-h1, Df(2L)E110, Df(2L)Mdh, Df(2R)or-BR6, Df(3L)Ar14-8, Df(2L)cl-h3, Df(2R)Pu-D17, Df(3L)vin6, lid^k06801, lid¹⁰⁴²⁴, E(Pc)¹, E(Pc)², Su(z)2¹, Psc¹, Asx^XF23, Asx³, Asx¹³, Sce^D1, E(z)⁵, Trl^R85, Su(z)7¹, Su(z)6¹, Scm^D1 or Scm^M56 show T3 to T2 transformations.

(Gildea et al., 2000)

The addition of ash1²² does not affect the variable eye size phenotype seen in osa^Scer\UAS.cCa/Scer\GAL4^ey.PH flies.

(Collins et al., 1999)

The eye colour of flies carrying w^{4.Ubx.bxd:pbx.N} is strongly reduced if they are also heterozygous for ash1²².

(Rozovskaia et al., 1999)

Xenogenetic Interactions

Statement

Reference

(Huang et al., 2017)

Complementation and Rescue Data

Rescued by

FBal0351313:, /Df(3L)Exel9011/ash1²² is rescued by ash1^Ubi-p63E.FL/ash1^Ubi-p63E.FL

(Dorafshan et al., 2019)

FBal0351314:, /Df(3L)Exel9011/ash1²² is rescued by ash1^{ΔPHD.Ubi-p63E}/ash1^{ΔPHD.Ubi-p63E}

(Dorafshan et al., 2019)

FBal0351313:, /Df(3L)Exel9011/ash1²² is rescued by ash1^Ubi-p63E.FL/ash1^Ubi-p63E.FL

(Dorafshan et al., 2019)

Df(3L)Exel9011/ash1²² is rescued by ash1^{ΔAT.Ubi-p63E}

(Dorafshan et al., 2019)

FBal0356963:, /Df(3L)Exel9011/ash1²² is rescued by ash1^{ΔAT.Ubi-p63E}/ash1^{ΔAT.Ubi-p63E}

(Dorafshan et al., 2019)

Df(3L)Exel9011/ash1²² is rescued by ash1^Ubi-p63E.FL

(Dorafshan et al., 2019)

ash1²² is rescued by ash1^C.Tag:TAP

(Schmähling et al., 2018)

ash1²² is rescued by ash1^N.Tag:TAP

(Schmähling et al., 2018)

ash1^R1288A/ash1²² is rescued by ash1^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

ash1²²/ash1¹⁰ is rescued by ash1^{αTub84B.EGFP}

(Steffen et al., 2013)

Df(3L)Exel9011/ash1²² is rescued by ash1^{αTub84B.EGFP}

(Steffen et al., 2013)

Partially rescued by

FBal0351314:, /Df(3L)Exel9011/ash1²² is partially rescued by ash1^{ΔPHD.Ubi-p63E}/ash1^{ΔPHD.Ubi-p63E}

(Dorafshan et al., 2019)

FBal0351315:, /Df(3L)Exel9011/ash1²² is partially rescued by ash1^{ΔSET.Ubi-p63E}/ash1^{ΔSET.Ubi-p63E}

(Dorafshan et al., 2019)

FBal0351315:, /Df(3L)Exel9011/ash1²² is partially rescued by ash1^{ΔSET.Ubi-p63E}/ash1^{ΔSET.Ubi-p63E}

(Dorafshan et al., 2019)

FBal0356961:, /Df(3L)Exel9011/ash1²² is partially rescued by ash1^{ΔBAH.Ubi-p63E}/ash1^{ΔBAH.Ubi-p63E}

(Dorafshan et al., 2019)

Df(3L)Exel9011/ash1²² is partially rescued by ash1^{ΔPHD.Ubi-p63E}

(Dorafshan et al., 2019)

Df(3L)Exel9011/ash1²² is partially rescued by ash1^{ΔSET.Ubi-p63E}

(Dorafshan et al., 2019)

ash1²² is partially rescued by ash1^+t9.5

(Schmähling et al., 2018)

ash1²² is partially rescued by ash1^R1464A

(Schmähling et al., 2018)

Not rescued by

Df(3L)Exel9011/ash1²² is not rescued by ash1^{ΔBAH.Ubi-p63E}

(Dorafshan et al., 2019)

Comments

Either ash1^{ΔSET.Ubi-p63E} or ash1^{ΔPHD.Ubi-p63E} homozygosity rescue ash1²²/Df(3L)Exel9011 transheterozygotes to adulthood. However, adults have low fitness, with no stable stocks being established, and all individuals show homeotic transformation.

(Dorafshan et al., 2019)

ash1²²/Df(3L)Exel9011 individuals rescued by ash1^Ubi-p63E.FL or ash1^{ΔAT.Ubi-p63E} are viable, fertile and do not show homeotic transformations.

(Dorafshan et al., 2019)

ash1²²/ash1²² adults expressing ash1^C.Tag:TAP or ash1^N.Tag:TAP are morphologically normal compared to controls.

ash1^+t9.5/+ expressing ash1²²/ash1²² animals derived from ash1²²/ash1²² mothers are fertile and morphologically normal compared to controls.

ash1^R1464A expressing ash1²²/ash1²² animals that contain maternally deposited wild-type Ash1 protein and zygotically expressed Ash1 protein with the R1464A point mutation are fertile.

ash1^R1464A/+ expressing ash1²²/ash1²² animals that contain maternally deposited and zygotically expressed Ash1 protein with the R1464A point mutation exhibit similar homeotic transformations as ash1²²/ash1²² pharate adults. A substantial fraction of them eclose from the pupal case but die in 1-2 days.

(Schmähling et al., 2018)

Both the third leg to second leg partial transformation and the haltere to wing partial transformation exhibited by ash1^R1288A/ash1²² transheterozygotes are rescued by the expression of ash1^Scer\UAS.cHa under the control of Scer\GAL4^arm.PS.

(Huang et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

24161

w^*; ash1²² P{FRT(w^hs)}2A/TM6C, Sb¹ Tb¹

Notes on Origin

Discoverer

Comments

Range of lethality for transheterozygotes extends from the third larval instar for the most severe alleles to the pharate adult stage for the least severe alleles.

(Tripoulas et al., 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Ash1²²

(Kirilly et al., 2011)

VV183

ash1²²

(Steffen et al., 2021, Dorafshan et al., 2019, Dorafshan et al., 2019, Schmähling et al., 2018, Song et al., 2018, Huang et al., 2017, McCracken and Locke, 2016, Copur and Müller, 2013, Steffen et al., 2013, Popkova et al., 2012, Beltran et al., 2007, Papp and Muller, 2006)

ash1^VV183

(Cheng and Shearn, 2004, Gildea et al., 2000)

ash1^vv183

(Decoville et al., 2001, Lopez et al., 2001)

dash²²

(Lindsley and Zimm, 1992)

dash^VV183

(Shearn et al., 1971)

Name Synonyms

Secondary FlyBase IDs

References (30)

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