FB2024_03 , released June 25, 2024
Allele: Dmel\Dll3
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General Information
Symbol
Dmel\Dll3
Species
D. melanogaster
Name
FlyBase ID
FBal0001001
Feature type
allele
Associated gene
Dmel\Dll
Associated Insertion(s)
Carried in Construct
Key Links
Allele class

hypomorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - genetic evidence

(Gorfinkiel et al., 1999, Couso and Bishop, 1998, Cohen and Jurgens, 1989)
Mutagen
ethyl methanesulfonate
(Cohen and Jurgens, 1989, Sunkel and Whittle, 1987)
Progenitor genotype
Cytology

Polytene chromosomes normal.

(Sunkel and Whittle, 1987)
Description
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      adult hindgut

      (Gorfinkiel et al., 1999)

      antenna

      (Dong et al., 2000)

      antenna (with Dll7)

      (Dey et al., 2009, Dong et al., 2002)

      antenna (with Dllmd23)

      (Dey et al., 2009)

      antennal segment 2 & macrochaeta

      (Sunkel and Whittle, 1987)

      arista

      (Dong et al., 2000, Sunkel and Whittle, 1987)

      arista | proximal

      (Dong et al., 2000)

      cercus | male

      (Gorfinkiel et al., 1999)

      dorsal organ | larval stage (with Dll1)

      (Plavicki et al., 2012)

      dorsal organ | larval stage (with Dll17)

      (Plavicki et al., 2012)

      femur

      (Dong et al., 2000, Campbell and Tomlinson, 1998, Couso and Bishop, 1998)

      femur & macrochaeta

      (Sunkel and Whittle, 1987)

      hypogynium

      (Gorfinkiel et al., 1999)

      Kenyon cell | embryonic stage 12 (with Dll7)

      (Plavicki et al., 2012)

      leg

      (Dong et al., 2000, Couso and Bishop, 1998)

      leg | ectopic

      (Dong et al., 2000)

      leg | ectopic (with Dll7)

      (Dong et al., 2002)

      leg | ectopic (with Dllmd23)

      (Dong et al., 2002)

      macrochaeta | ectopic

      (Sunkel and Whittle, 1987)

      mushroom body medial lobe | embryonic stage 12 (with Dll7)

      (Plavicki et al., 2012)

      mushroom body pedunculus | embryonic stage 12 (with Dll7)

      (Plavicki et al., 2012)

      mushroom body vertical lobe | embryonic stage 12 (with Dll7)

      (Plavicki et al., 2012)

      second segment of antenna

      tarsal segment

      (Dong et al., 2000, Campbell and Tomlinson, 1998)

      tarsal segment (with Dll7)

      (Dey et al., 2009)

      tarsal segment (with Dllmd23)

      (Dey et al., 2009)

      third segment of antenna

      (Dong et al., 2000, Sunkel and Whittle, 1987)

      third segment of antenna (with Dllmd23)

      (Dong et al., 2002)

      third segment of antenna | distal

      (Dong et al., 2000)

      third segment of antenna | distal (with Dll7)

      (Dong et al., 2002)

      tibia

      (Dong et al., 2000, Campbell and Tomlinson, 1998, Couso and Bishop, 1998)

      tibia & macrochaeta

      tibia | ectopic

      (Dong et al., 2000)

      trochanter

      (Couso and Bishop, 1998)

      unguis (with Dll7)

      (Dey et al., 2009)
      Detailed Description
      Statement
      Reference

      Dll17/Dll3 mutant larvae show a significantly impaired behavioural response to an attractive odorant (EtOAc). Some larvae show dorsal organ malformations but there appears to be no correlation between the larvae with cuticular defects and those with olfactory impairment. Motor activity appears normal, and allowing more time for the larva to respond (10 minutes rather than 5 minutes) does not change the response indices.

      Dll3/Dll7 mutant stage 12 embryos show disruption to the mushroom body peduncles. The Kenyon cell clusters are reduced in size by at least 50%, the calyces are markedly smaller, the dorsal and medial lobes are thinned and the relative position of the peduncle and the dorsal lobe are abnormal.

      Dll1/Dll3 mutant larvae have malformed dorsal organs. Larvae exhibit a normal behavioural response to an attractive odorant.

      (Plavicki et al., 2012)

      Dll3/Dll7 animals display antenna to leg transformations and deletion of tarsal segments 3-5 and the claw.

      About 3% of Dllmd23/Dll3 animals emerge as adults, with all escapers displaying partial transformation of the antenna to leg structures and deletion of distal leg structures.

      (Dey et al., 2009)

      The distal antennal segment 3 (a3) and the arista of Dll3/Dll7 transheterozygotes are transformed towards leg. The circular outline of the joint between antennal segments 2 and 3 is also missing. Dllmd23/Dll3 animals results in the loss of the arista and transformation of antennal segment 3 towards leg. Antennal segment 2 is present and has a normal cuticle.

      (Dong et al., 2002)

      Dll3/Dll7 animals show partial transformation of the antenna into leg. The distal part of antennal segment 3 and the proximal part of the arista are transformed towards tibia. Tarsal segment deletions and fusions in the legs are seen. Dll1/Dll3 animals die as pharate adults. They show both a transformation of the antenna towards leg and distal truncations. What remains of antennal segment 3 resembles leg tissue and the arista is deleted. A complete loss of the tarsal segments and shortening of both the femur and tibia is seen in the legs. Dll3/Dll17 animals die as pharate adults. They show truncations of the antenna, such as deletion of antennal segment 3 and the arista. A complete loss of the tarsal segments and shortening of both the femur and tibia is seen in the legs.

      (Dong et al., 2000)

      In Dll3/Df(2R)Dll-MP adults, the hindgut is enlarged at the expense of the anal plates in both males and females. In females, the vaginal plates are disorganised. A few anal bristles surrounded by hindgut tissue may be observed. The internal and external genitalia appear normal in both males and females.

      (Gorfinkiel et al., 1999)

      Homozygotes lack all of the tarsus and the tibia and femur are reduced in size.

      (Campbell and Tomlinson, 1998)

      Homozygous adults have legs that are truncated at the tibia. The tibia, femur and trochanter are shorter than wild-type and are deformed.

      (Couso and Bishop, 1998)

      Severe leg reductions: deletion of all distal tarsal segments, tibia and femur very reduced.

      (Cohen and Jurgens, 1989)

      Greatly reduced femur and tibia, associated with normal trochanter and coxa.

      (Cohen et al., 1989)

      Heterozygotes are wild-type. Homozygotes die as pharate adults in which the most distal part of the arista is sometimes present, but there is no evidence of a third antennal segment. The second segment has a large number of ectopic bristles, some of which are bracted. The thoracic legs are distorted and lack all elements distal to the tibia. Duplications are sometimes seen in the femur which have a large number of ectopic bristles that are not bracted. Hemizygous but not homozygous embryos sometimes lack Keilin's organs and the labial sense organ.

      (Sunkel and Whittle, 1987)

      Dll3/+ wild type; Dll3/Dll1 fail to eclose; pharate adults lack third antennal segment; second segment transformed to leg; distal arista normal. Legs lack all structures distal to tibiae; tibiae enlarge and bear ectopic bristles; no remaining leg bristles are bracted. recessive pharate-adult lethal

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Statement
      Reference

      Dll3/Dllmd23 has lethal phenotype, enhanceable by discoRNAi.UAS/Scer\GAL4Dll-md23

      (Dey et al., 2009)
      Suppressed by
      Statement
      Reference

      Dll3/Dllmd23 has lethal phenotype, suppressible | partially by discoUAS.cDa/Scer\GAL4Dll-md23

      (Dey et al., 2009)

      Dll3/Dllmd23 has homeotic phenotype, suppressible | partially by discoUAS.cDa/Scer\GAL4Dll-md23

      (Dey et al., 2009)
      Phenotype Manifest In
      Suppressed by
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Scer\GAL4Dll-md23-mediated expression of discoScer\UAS.cDa partially rescues the lethality associated with Dllmd23/Dll3, and nearly completely rescues the antenna and leg phenotypes of this mutant.

      Scer\GAL4Dll-md23-mediated expression of discodsRNA.Scer\UAS reduces the number of Dllmd23/Dll3 escapers to nearly zero.

      (Dey et al., 2009)

      No effect on the eye pigment phenotype of wT81.

      (Netter et al., 1998)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Dll3 is rescued by Scer\GAL4Dll-em212/DllUAS.cGa

      (Gorfinkiel et al., 1997)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Sunkel.

      Comments
      Comments

      Strong Dll allele.

      (Cohen et al., 1989)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Ba3
      (Lindsley and Zimm, 1992)
      Dll3
      (Ahn et al., 2011, Dey et al., 2009, Galindo et al., 2005)
      dll3
      (Plavicki et al., 2012)
      Name Synonyms
      Secondary FlyBase IDs
        References (21)