FlyBase Allele Report: Dmel\bi[omb-3198]

General Information

Symbol

Dmel\bi^omb-3198

Species

D. melanogaster

Name

FlyBase ID

FBal0001123

Feature type

allele

Associated gene

Dmel\bi

Associated Insertion(s)

Carried in Construct

Also Known As

l(1)omb³¹⁹⁸, omb³¹⁹⁸

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Tomlinson, 2003, Kopp and Duncan, 1997)

Mutagen

ethyl methanesulfonate

(Poeck et al., 1993, Pflugfelder et al., 1990)

Progenitor genotype

Cytology

Description

Point mutation causing premature termination of the protein.

(Tsai et al., 1997)

Point mutation.

(Balles and Pflugfelder, 1994, Pflugfelder et al., 1992)

Amino acid replacement: W395term.

(Poeck et al., 1993)

Nucleotide substitution: G?A.

(Poeck et al., 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:4,450,882..4,450,882 [+]

Nucleotide change:

G4450882A

Reported nucleotide change:

G?A

Amino acid change:

Reported amino acid change:

W395term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

(Poeck et al., 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal tergite | posterior & trichome

(Kopp and Duncan, 1997)

adult cuticle & abdomen | somatic clone | cell autonomous

(Lawrence et al., 2002)

anterior-posterior compartment boundary of the wing disc | somatic clone

(Shen and Dahmann, 2005)

microchaeta | somatic clone

(Lawrence et al., 2002)

tergite | posterior

(Kopp and Duncan, 1997)

wing

(del Alamo Rodriguez et al., 2004)

wing disc

(del Alamo Rodriguez et al., 2004)

Detailed Description

Statement

Reference

bi^omb-3198 clones generated along the A/P boundary of the wing imaginal disc, using the FLP/FRT technique, show a defective segregation phenotype. bi^omb-3198 clones of A, but not P, origin are partly misplaced into the territory of the adjacent compartment, causing a displacement of the A/P compartment boundary toward P.

(Shen and Dahmann, 2005)

Mutants die as pharate adults. A deletion of the central wing disc is seen, also huge overgrowths are seen that do not evaginate, but remain internal.

(del Alamo Rodriguez et al., 2004)

Clones of bi^omb-3198 that are induced in the dorsal half of the eye do not remove the dorsal rim ommatidia.

(Tomlinson, 2003)

When somatic clones are made in the segmental anterior 4 (a4) region of the anterior segmental compartment in the adult abdomen an associated patch of reversed polarity is seen, which can extend in front of and behind the clone. The clone itself also lacks the dark a4 pigment. When the clone is near the back of the anterior compartment hairs (and thus polarity) are reversed. This reversed polarity effect only extends as far as the anterior posterior boundary. When somatic clones are made in the segmental posterior 3 (p3) region of the posterior segmental compartment in the adult abdomen, about one third of the clones lost some, but not all of the hairs within the clone.

(Lawrence et al., 2002)

Hemizygotes usually die at the late larval or early pupal stage, with a few animals surviving to the late pharate adult stage. Homozygous clones in the abdomen have no effect on polarity or cell fates when located in the anterior tergite. Clones in the posterior tergite and posterior hairy zone cause reversal of trichome polarity and disappearance of the pigment band. Clones in the posterior tergite are small, even if induced at early embryonic stages.

(Kopp and Duncan, 1997)

Neuroanatomical omb phenotype.

(Poeck et al., 1993)

Displays lac and rb phenotypes.

(Pflugfelder et al., 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

bi^omb-3198 is a non-suppressor of increased cell number | somatic clone phenotype of Scer\GAL4^Act5C.PP, tkv^{Q253D.UAS.cNc}

(Martin-Castellanos and Edgar, 2002)

bi^omb-3198 is a non-suppressor of increased occurrence of cell division | somatic clone | larval stage phenotype of Scer\GAL4^Act5C.PP, tkv^{Q253D.UAS.cNc}

(Martin-Castellanos and Edgar, 2002)

Phenotype Manifest In

Suppressed by

Statement

Reference

bi^omb-3198 has anterior-posterior compartment boundary of the wing disc | somatic clone phenotype, suppressible by ci^UAS.cDa/Scer\GAL4^hh-Gal4

(Shen and Dahmann, 2005)

bi^omb-3198 has wing disc anterior compartment | somatic clone phenotype, suppressible by ci^UAS.cDa/Scer\GAL4^hh-Gal4

(Shen and Dahmann, 2005)

bi^omb-3198 has wing disc phenotype, suppressible by Scer\GAL4^nub-AC-62/puc^UAS.cMa

(del Alamo Rodriguez et al., 2004)

NOT suppressed by

Statement

Reference

bi^omb-3198 has wing disc phenotype, non-suppressible by Scer\GAL4^nub-AC-62/puc^UAS.cMa

(del Alamo Rodriguez et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Clones generated along the A/P boundary of the wing imaginal disc, that overexpress ci^Scer\UAS.cDa, driven by Scer\GAL4^hh-Gal4, in a bi^omb-3198 background show no mis-segregation phenotype.

(Shen and Dahmann, 2005)

When bi^omb-3198 ptc¹⁶ double homozygous clones are made in the anterior compartment of the abdominal segment. These clones makes segmental region anterior 6 (a6) cuticle, like ptc¹⁶ clones, but reverse polarity in the front half of the clone as do bi^omb-3198 clones.

(Lawrence et al., 2002)

When tkv^{Q253D.Scer\UAS.cNc} is driven by Scer\GAL4^Act5C.PP in a bi^omb-3198 background, no suppression of the proliferation phenotype is seen.

(Martin-Castellanos and Edgar, 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

80152

y¹ w^* bi^omb-3198/FM7a

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 6 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

AAB26699

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Df(1)omb3198

bi^omb-3198

l(1)bi²

l(1)omb3198

(Shen et al., 2008)

l(1)omb³¹⁹⁸

(Reinhardt et al., 2019, Tsai et al., 2015, Kopp and Duncan, 1997, Tsai et al., 1997, Grimm and Pflugfelder, 1996, Balles and Pflugfelder, 1994, Pflugfelder et al., 1990)

omb^-

(Lawrence et al., 2002)

omb³¹⁹⁸

(Shen and Dahmann, 2005, del Alamo Rodriguez et al., 2004, Tomlinson, 2003, Takatsu et al., 2000, Poeck et al., 1993, Pflugfelder et al., 1992)

Name Synonyms

Secondary FlyBase IDs

FBab0000866

References (16)

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