microtubule & oocyte (with BicC3)
nurse cell (with BicC3)
5% of eggs laid by heterozygous females have shortened or fused dorsal appendages.
A zone along the anterior region of the oocyte shows a significantly higher concentration of microtubules in BicC3/BicC4 egg chambers compared to wild type.
BicC3/BicC4 egg chambers often show incomplete nurse cell dumping. The actin cables that normally restrain the nurse cell nuclei are absent in the mutant egg chambers, and nurse cell nuclei are often seen to have entered the ring canals.
Dominant haplo-insufficient phenotype. All egg chambers are open ended, heterozygous females produce low frequency of bicaudal embryos.
Phenotypically wild type embryos from heterozygous mothers localize vas protein normally throughout embryogenesis. In phenotypically bicaudal embryos the pole cells fail to coalesce into gonads.
BicC4 has dorsal appendage | maternal effect phenotype, enhanceable by tral[+]/tralKG08052
BicC4 has dorsal appendage | maternal effect phenotype, enhanceable by tral[+]/tral1Δ1
BicC4 has dorsal appendage | maternal effect phenotype, enhanceable by me31BΔ1/me31B[+]
BicC4 has dorsal appendage | maternal effect phenotype, enhanceable by exu1/exu[+]
BicC4 has dorsal appendage | maternal effect phenotype, non-enhanceable by swa[+]/swa1
The penetrance of the mutant dorsal appendage phenotype seen in eggs laid by BicC4/+ females (5%) is dramatically increased if the females also carry tralKG08052/+ (97%), tral1Δ1/+ (65%), me31BΔ1/+ (79%) or exu1/+ (24%).
cupΔ212/+ has a very weak effect on the penetrance of the mutant dorsal appendage phenotype seen in eggs laid by BicC4/+ females, increasing it from 5% to 9% in the double heterozygous flies.
swa1/+ does not increase the penetrance of the mutant dorsal appendage phenotype seen in eggs laid by BicC4/+ females.
Schupbach.
T. Schupbach.
