eag4PM mutant larvae fail to exhibit Response Index increment by associative training upon LIN/SUC exposure.
The naive olfactory response of eag4PM larvae is slightly higher than in wild-type but not statistically significant. The gustatory response for SUC is slightly higher in eag4PM larvae than in wild-type flies.
The rate of habituation of the giant fiber escape pathway is increased and the number of stimuli needed to attain five consecutive failures is decreased in eag4PM flies compared to wild-type. The short-latency response of the dorsal longitudinal muscle occurs earlier than in wild-type flies.
Hemizygotes fail to show courtship conditioning, showing an increase in courtship of mated females compared to wild type controls.
Response of the channel to modulation by protein kinases depends on temperature: IA is increased at 5oC but remains almost unchanged at 16oC, conversely IK is nearly normal at 5oC and decreased at 16oC. At 16oC IA and IK levels, and ICF and ICS levels can be reduced by application of W7, a Ca2+/CaM antagonist. cGMP-modulation of IK and IA by the mutant channel by 8-Br-cGMP is altered.
Flies show some leg-shaking under anaesthesia. The action potential of the dorsal longitudinal muscle occasionally appears abnormal in eag4PM mutants. Lowers the following frequency of the giant fibre response of the tergotrochanteral muscle pathway. eag4PM Sh21 but not eag4PM Sh5 double mutant flies show spontaneous activity of the dorsal longitudinal and dorsoventral indirect flight muscles when at rest. This phenotype is suppressed by mlenap-ts1 in eag4PM Sh21 mlenap-ts1 flies. 54% of eag4PM Sh21 flies have a wings-down phenotype.
Defects in all K+ currents within larval muscle.
eag4PM Sh21 double mutant larvae show an increased ramification of neurites throughout muscles 12 and 13. This phenotype is suppressed by mlenap-ts1 at the restrictive temperature. eag4PM Sh21 double mutant larval muscles show periods of activity not organised into bursts (tonic activity) in contrast to wild-type. This phenotype is partially suppressed by mlenap-ts1.
Flies show slight leg-shaking behaviour.
weak behavioral abnormalities but is abnormal physiologically;
eag4PM has increased rate of movement phenotype, enhanceable by qvr1
eag4PM is an enhancer of increased rate of movement phenotype of qvr1
Sh21, eag4PM has abnormal behavior phenotype
eag4PM Sh21 and eag1 Sh21 double mutants exhibit vigorous leg-shaking behaviour under ether anaesthesia. Many exhibit a wings down phenotype. High frequency, spontaneous EJPs of a greater amplitude are seen at larval neuromuscular junctions and spontaneous discharges of EJPs or action potentials can be recorded in adult flight muscles.
