FlyBase Allele Report: Dmel\Gpdh1[n1-4]

General Information

Symbol

Dmel\Gpdh1^n1-4

Species

D. melanogaster

Name

FlyBase ID

FBal0005144

Feature type

allele

Associated gene

Dmel\Gpdh1

Associated Insertion(s)

Carried in Construct

Also Known As

n1-4

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(O'Brien, 1972)

Mutagen

ethyl methanesulfonate

(O'Brien, 1972)

Progenitor genotype

Gpdh1^B

(O'Brien, 1972)

Cytology

Description

Nucleotide substitution: G?A.

(Carmon et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:5,946,338..5,946,338 [+]

Nucleotide change:

G5946338A

Reported nucleotide change:

G?A

Amino acid change:

Reported amino acid change:

G151D

Comment:

Reported as Gly to Asn, GGC to GAC. Amino acid change presumed to be a typo. Should be Gly to Asp.

(Carmon et al., 2010)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Kaltenbach et al., 2007)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

flightless

(O'Brien, 1972)

viable

(O'Brien, 1972)

Phenotype Manifest In

Detailed Description

Statement

Reference

Mutant flies do not show significantly increased survival compared to wild-type flies after infection with E. chaffeensis.

(Von Ohlen et al., 2012)

Adults are visibly normal but cannot sustain flight.

(O'Brien, 1972)

%CRM by rocket electrophoresis: >5 % wildtype enzyme activity: 1 relative enzyme mobility: >Gpdh^B relative viability: .20 flightless

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Gpdh1^n1-4 is an enhancer of visible | pharate adult stage phenotype of Gk1^RNAi.UAS, Scer\GAL4^c564

(Wightman et al., 2013)

Suppressor of

Statement

Reference

Gpdh1^n1-4 is a suppressor of visible phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Kaltenbach et al., 2007)

Phenotype Manifest In

Enhancer of

Statement

Reference

Gpdh1^n1-4 is an enhancer of wing | pharate adult stage phenotype of Gk1^RNAi.UAS, Scer\GAL4^c564

(Wightman et al., 2013)

Suppressor of

Statement

Reference

Gpdh1^n1-4 is a suppressor of eye phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Kaltenbach et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Gpdh^n1-4 enhances the curled or crumpled wing phenotype seen in Scer\GAL4^c564>Gk1^{dsRNA.Scer\UAS}-let escapers.

(Wightman et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

1124

al¹ Gpdh1^n1-4/SM1

Notes on Origin

Discoverer

S.J. O'Brien.

(O'Brien, 1972)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Gpdh1^n1-4

Gpdh^B0-1-4

Gpdh^n1-4

(Wightman et al., 2013)

n1-4

(Carmon et al., 2010, Kaltenbach et al., 2007)

α-GPDH-1^N1-4

(O'Brien, 1972)

Name Synonyms

negative 1-4

(O'Brien, 1972)

Secondary FlyBase IDs

References (8)

Report Sections

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