No mutations are found in the jv coding region. Transcript levels are reduced 4.9-fold compared to wild type.
jv1 macrochaetes are abnormally smooth, with shallower and thinner ridges than in wild type. They are also bulbous at the tip, and the tip has abnormally organized surface grooves with the ridges not being parallel to each other. In 25% of jv1 files, the posterior scutellar bristles are fused. However, jv1 bristles are of wild type length.
At 42 h after puparium formation (APF), actin bundles within the developing bristles of jv1 mutants remain wide rather than tapering toward the tip. At 48 h APF, the mutant bundles are disordered and run in different directions along the bristle shaft and some appear to be thinner than others. These defects are more severe at the swollen bristle tip. In transverse section, the mutant actin bundles are not triangular in shape as in wild type. Also , there are gaps in some bundles when no actin filaments are observed. In some extreme cases, the mutant bundles are larger than wild type owing to the more disordered arrangement of the filaments.
The microtubule array is properly organized in jv1 bristle shafts.
jvc03230/jv1 show defects in bristle development similar to jv1.
Denticles tend to be a little thin and elongate but this is variable and difficult to reliably distinguish from wild type.
Cell autonomous and useful as a marker for epidermal clones.
RK2. All bristles and hairs cylindrical, instead of tapered, with small enlargement before tip.
jv1 is partially rescued by jvUASp.GFP/Scer\GAL4Act5C.PU
jv1/jvc03230 is partially rescued by jvUASp.GFP/Scer\GAL4Act5C.PU
Scer\GAL4Act5C.PU-mediated expression of jvScer\UAS.P\T.T:Avic\GFP partially rescues the swollen bristle tip and fused posterior scutellar bristle phenotypes of jv1 and jv1/jvc03230 flies. Bristle ridges are more pronounced, though ridges still do not run parallel to each other.
