FlyBase Allele Report: Dmel\kn[1]

General Information

Symbol

Dmel\kn¹

Species

D. melanogaster

Name

FlyBase ID

FBal0005770

Feature type

allele

Associated gene

Dmel\kn

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

hypomorphic allele

Mutagen

Nature of the Allele

Allele class

hypomorphic allele

(Chao et al., 2017)

Mutagen

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

1st posterior cell

(Mohler et al., 2000)

1st posterior cell (with kn^col-1)

(Crozatier et al., 2002)

2nd posterior cell (with kn^col-1)

(Crozatier et al., 2002)

3rd posterior cell (with kn^col-1)

(Crozatier et al., 2002)

anterior crossvein

(Vervoort et al., 1999)

dendrite | somatic clone

(Jinushi-Nakao et al., 2007)

dendrite | somatic clone (with kn^KN2)

(Jinushi-Nakao et al., 2007)

larval dorsal multidendritic neuron ddaC | somatic clone

(Jinushi-Nakao et al., 2007)

larval dorsal multidendritic neuron ddaC | somatic clone (with kn^KN2)

(Jinushi-Nakao et al., 2007)

larval multidendritic class IV neuron | somatic clone

(Jinushi-Nakao et al., 2007)

larval multidendritic class IV neuron | somatic clone (with kn^KN2)

(Jinushi-Nakao et al., 2007)

wing | P-stage (with kn^col-1)

(Crozatier et al., 2002)

wing basal cell 1

(Mohler et al., 2000)

wing cell & wing vein L3 & wing vein L4

(Nestoras et al., 1997)

wing vein L3

(Mohler et al., 2000, Vervoort et al., 1999)

wing vein L4

(Mohler et al., 2000, Vervoort et al., 1999)

Detailed Description

Statement

Reference

kn¹ MARCM clones exhibit many termini in tufts at the ends of the main dendrite branches.

kn¹/kn^KN2 mutants exhibit 59% more uncovered areas of dendritic outgrowth compared to wild-type ddaC neurons.

kn¹/kn^KN2 ddaC neurons exhibit a significant reduction in dendrite termini to 77% of wild-type levels. In addition there is a polarization of the normally symmetrical class IV dendritic arbor. In mutant neurons about 70% of the dendrite termini are concentrated in the region dorsal to the cell body.

kn¹/kn^KN2 mutant ddaC neuronal clones display a marked reduction of dendrite outgrowth and branching, with tufts of short terminal dendrite branches at the ends of the main branch segments often being visible. The termini are concentrated nearer to the cell body than in wild-type.

kn¹/kn^KN2 mutants do not survive into late embryonic stages.

(Jinushi-Nakao et al., 2007)

Wings veins L3 and L4 are closer to each other in kn^col-1/kn¹ animals than in wild type and are apposed proximally. The L3-L4 intervein is 66+/-6% smaller than wild type, whereas the anterior margin to L2 an dL2-L3 interveins are normal in size. The L4-L5 and L5 to posterior margin interveins are smaller than wild type. The wave of mitosis that normally takes place in each intervein primordium between 15 and 21 hours after puparium formation is specifically absent from the central region of kn^col-1/kn¹ wings.

(Crozatier et al., 2002)

kn¹/kn^SA1 flies have wings that lack most of the intervein space betweens veins 3 and 4, and show fusions of veins 3 and 4, particularly proximal to the posterior crossvein.

(Mohler et al., 2000)

In a kn¹/kn^col-1 transheterozygote, the proximal regions of wing veins L3 and L4 are apposed, and the anterior crossvein is missing.

(Vervoort et al., 1999)

Fusion of wing veins L3 and L4 in the position of the anterior cross vein and narrowing of the more distal intervein space. Transheterozygotes with kn^KN1, kn^KN2, kn^KN3 or kn^KN4 enhance the wing vein phenotype, the veins are extensively fused. This loss of intervein region reduced the length and width of the wings by 80-90%. The transition point between socketed and unsocketed bristles at the wing margin is shifted posteriorly to the shifted position of wing vein L3.

(Nestoras et al., 1997)

Narrow wings with short connecting extra veins between LIII and LIV.

(Diaz-Benjumea and Garcia-Bellido, 1990)

Veins L3 and L4 shifted closer together in region of anterior crossvein, which is either extremely thick or eliminated by regional fusion of L3 and L4. Frequently, extra crossvein between L3 and L4 near end of wing. Shift in positions of sensilla and extra chaetae accompanies shift in vein positions. Wing narrowed. Head narrowed and flattened, so the long axis of eye is at oblique angle. May overlap wild type at high temperatures and in late counts. Best at 19^oC. RK2.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

kn^KN2/kn¹ has abnormal neuroanatomy | somatic clone phenotype, enhanceable by ct^UAS.cPa/Scer\GAL4^ppk.PG

(Jinushi-Nakao et al., 2007)

kn¹/kn^col-1 has visible phenotype, enhanceable by hh^ts2

(Vervoort et al., 1999)

Suppressor of

Statement

Reference

kn¹ is a suppressor of visible phenotype of ptc^G20

(Nestoras et al., 1997)

Other

Statement

Reference

kn¹, vn[+]/vn^M2 has visible phenotype

(Crozatier et al., 2002)

kn¹, vn^M2 has visible phenotype

(Crozatier et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

kn^KN2/kn¹ has larval dorsal multidendritic neuron ddaC | somatic clone phenotype, enhanceable by ct^UAS.cPa/Scer\GAL4^ppk.PG

(Jinushi-Nakao et al., 2007)

kn^KN2/kn¹ has dendrite | somatic clone phenotype, enhanceable by ct^UAS.cPa/Scer\GAL4^ppk.PG

(Jinushi-Nakao et al., 2007)

kn^KN2/kn¹ has larval multidendritic class IV neuron | somatic clone phenotype, enhanceable by ct^UAS.cPa/Scer\GAL4^ppk.PG

(Jinushi-Nakao et al., 2007)

kn¹/kn^col-1 has wing vein phenotype, enhanceable by hh^ts2

(Vervoort et al., 1999)

kn¹/kn^col-1 has wing vein L3 phenotype, enhanceable by hh^ts2

(Vervoort et al., 1999)

kn¹/kn^col-1 has wing vein L4 phenotype, enhanceable by hh^ts2

(Vervoort et al., 1999)

Suppressed by

Statement

Reference

kn¹ has phenotype, suppressible by Egfr^t1/Egfr^f11

(Diaz-Benjumea and Garcia-Bellido, 1990)

kn¹ has wing vein phenotype, suppressible by Egfr^t1/Egfr^f11

(Diaz-Benjumea and Garcia-Bellido, 1990)

Suppressor of

Statement

Reference

kn¹ is a suppressor of wing vein L2 phenotype of ptc^G20

(Nestoras et al., 1997)

kn¹/kn¹ is a suppressor of phenotype of Egfr^t1/Egfr^f11

(Diaz-Benjumea and Garcia-Bellido, 1990)

kn¹/kn¹ is a suppressor of wing vein phenotype of Egfr^t1/Egfr^f11

(Diaz-Benjumea and Garcia-Bellido, 1990)

Other

Statement

Reference

kn¹, vn[+]/vn^M2 has wing vein L4 phenotype

(Crozatier et al., 2002)

kn¹, vn^M2 has wing vein L4 phenotype

(Crozatier et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of ct^Scer\UAS.cPa under the control of Scer\GAL4^ppk.PG in class IV neurons in a kn¹/kn^KN2 results in the formation of many additional filpodia/spikes that closely resemble those of class III neurons. The overall level of branching in class IV neuron dendritic arbors appears similar to those of class III neurons.

(Jinushi-Nakao et al., 2007)

kn¹/kn¹ ; vn^M2/+ flies lack most of wing vein L4, except for its most proximal and distal parts.

(Crozatier et al., 2002)

At 25^oC hh^ts2 enhances the wing vein phenotype seen in kn^col-1/kn¹.

(Vervoort et al., 1999)

Loss of wing vein L2 in ptc^G20 mutants is suppressed when simultaneously mutant for kn.

(Nestoras et al., 1997)

Double mutant combinations of Egfr^t1/Egfr^f11 with kn¹/kn¹ show mutual suppression, with both the Egfr and vein phenotypes being normalised.

(Diaz-Benjumea and Garcia-Bellido, 1990)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

kn^col-1

(Vervoort et al., 1999)

Rescued by

kn¹/kn^col-1 is rescued by kn^UAS.cVa/Scer\GAL4^dpp.blk1

(Vervoort et al., 1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

64235

kn¹/SM6a

Kyoto

105853

kn¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

(Diaz-Benjumea and Garcia-Bellido, 1990)

kn¹

(Yoong et al., 2020, Chao et al., 2017, Karim and Moore, 2011, Christensen et al., 2009.7.22, Christensen et al., 2008.4.15, Jinushi-Nakao et al., 2007, Glise et al., 2005, Crozatier et al., 2002)

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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