FlyBase Allele Report: Dmel\Cka[05836]

General Information

Symbol

Dmel\Cka⁰⁵⁸³⁶

Species

D. melanogaster

Name

FlyBase ID

FBal0008096

Feature type

allele

Associated gene

Dmel\Cka

Associated Insertion(s)

P{PZ}Cka⁰⁵⁸³⁶

Carried in Construct

Also Known As

l(2)05836, cka¹

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

Progenitor genotype

Associated Insertion(s)

P{PZ}Cka⁰⁵⁸³⁶

Cytology

Description

Insertion in the 5' untranslated region.

(Chen et al., 2002)

P{PZ} insertion in intron 1 of the CT22741 transcript of Cka, 359bp downstream of the 5' end of the transcript and 53bp downstream of the end of exon 1. P{PZ} element is inserted in the same orientation as the gene.

(Misra, 2000.11.18)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:8,031,104..8,031,104 [+]

(Liaw and Chiang, 2019, Chen et al., 2002, Misra, 2000.11.18, Spradling et al., 1999, Harvie et al., 1998, Russell et al., 1998, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Embryos lacking both maternal and zygotic Cka function (derived from females carrying homozygous germ-line clones) die during embryogenesis with a dorsal open phenotype. They have head defects and lack the dorsal epidermis, as indicated by a large dorsal hole in the cuticle. Embryos lacking both maternal and zygotic Cka function do not undergo the cell shape changes that normal occur in the leading edge and lateral epithelial cells during dorsal closure.

(Chen et al., 2002)

Cka⁰⁵⁸³⁶/Cka^s1883 mutant eye discs display a variable reduction in disc size. In some discs overall morphology appears normal. Malformed and misfolded discs are also observed, with a higher frequency in smaller discs.

(Weng and Du, 2002)

Homozygotes die during the larval and pupal stages. Embryos derived from homozygous female germ line clones have a dorsal open phenotype which can be fully paternally rescued.

(Russell et al., 1998)

Germline clones produce eggs with patterning defects: head and dorsal open. Lethality occurs during the larval and pupal stages.

(Perrimon et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Cka⁰⁵⁸³⁶ has embryonic dorsal epidermis | germline clone | rescuable maternal effect phenotype, suppressible by hep^CA.UAS/Scer\GAL4^hs.PB

(Chen et al., 2002)

Cka⁰⁵⁸³⁶ has embryonic dorsal epidermis | germline clone | rescuable maternal effect phenotype, suppressible by tkv^{Q199D.UAS.Tag:HA}/Scer\GAL4^hs.PB

(Chen et al., 2002)

Cka⁰⁵⁸³⁶ has embryonic dorsal epidermis | germline clone | rescuable maternal effect phenotype, suppressible by Jra^Asp.hs.sev

(Chen et al., 2002)

Enhancer of

Statement

Reference

Cka⁰⁵⁸³⁶/Cka[+] is an enhancer of embryonic dorsal epidermis phenotype of kay²

(Chen et al., 2002)

Cka⁰⁵⁸³⁶/Cka⁰⁵⁸³⁶ is an enhancer of embryonic dorsal epidermis phenotype of bsk¹

(Chen et al., 2002)

Suppressor of

Statement

Reference

Cka⁰⁵⁸³⁶/Cka[+] is a suppressor of eye phenotype of Ras85D^V12.sev

(Ashton-Beaucage et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The weak dorsal-open phenotype seen in homozygous kay² embryos is enhanced by Cka⁰⁵⁸³⁶/+. The weak dorsal-open phenotype seen in homozygous bsk¹ embryos is enhanced by Cka⁰⁵⁸³⁶/Cka⁰⁵⁸³⁶. The dorsal open phenotype seen in homozygous Cka⁰⁵⁸³⁶ embryos derived from females carrying homozygous Cka⁰⁵⁸³⁶ germ-line clones is substantially rescued by expression of Jra^Asp.hs.sev using heat shock. The dorsal open phenotype seen in homozygous Cka⁰⁵⁸³⁶ embryos derived from females carrying homozygous Cka⁰⁵⁸³⁶ germ-line clones is substantially rescued by expression of hep^CA.Scer\UAS under the control of Scer\GAL4^hs.PB using heat shock. The dorsal open phenotype seen in homozygous Cka⁰⁵⁸³⁶ embryos derived from females carrying homozygous Cka⁰⁵⁸³⁶ germ-line clones is substantially rescued by expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^hs.PB using heat shock.

(Chen et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Cka^s1883

(BDGP Project Members, 1994-1999)

Rescued by

Cka^{MI13708-TG4.0}/Cka⁰⁵⁸³⁶ is rescued by Cka^UASp.C.EGFP/Scer\GAL4^{Cka-MI13708-TG4.0}

(Lee et al., 2018)

Cka⁰⁵⁸³⁶ is rescued by Cka^hs.PC

(Chen et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11451

P{PZ}Cka⁰⁵⁸³⁶ cn¹/CyO; ry⁵⁰⁶

Notes on Origin

Discoverer

A. Spradling.

Comments

The P{EP}Cka^EP2514 insertion is 1bp away from the P{PZ}Cka⁰⁵⁸³⁶ insertion.

(Misra, 2000.11.18)

Precise excision of the insertion reverts the lethal mutant phenotype.

(Chen et al., 2002)

Complements: baf¹.

(Furukawa et al., 2003)

Complements: Df(2L)baf².

(Furukawa et al., 2003)

Complements: l(2)k10210^k10210.

(Furukawa et al., 2003)

Complements: mts⁰²⁴⁹⁶. Complements: Bsg⁰⁶²⁴³. Complements: Bsg^k04604. Complements: l(2)k06327^k06327. Complements: Bsg^k06338. Complements: Bsg^k07602. Complements: Bsg^k09030. Complements: Bsg^k09402. Complements: Bsg^k09412. Complements: l(2)k10210^k10210. Complements: l(2)k11101^k11101. Complements: Bsg^k13638. Complements: wg^rO727. Complements: l(2)s4199^s4199. Complements: poe⁰¹⁶⁵⁹. Fails to complement: l(2)k00607^k00607.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Cka⁰⁵⁸³⁶

(Neal et al., 2022, Liaw and Chiang, 2019, Lee et al., 2018, Liu et al., 2016)

Cka¹

(Ríos-Barrera et al., 2015, Ashton-Beaucage et al., 2014)

Cka²⁰³⁹

(Weng and Du, 2002)

WD-40-family-member⁰⁵⁸³⁶

cka¹

(Chen et al., 2002)

l(2)05836

(Furukawa et al., 2003, Chen et al., 2002, Misra, 2000.11.18, Spradling et al., 1999, Russell et al., 1998)

l(2)05836⁰⁵⁸³⁶

(Perrimon et al., 1996, BDGP Project Members, 1994-1999)

Name Synonyms

Secondary FlyBase IDs

References (17)

Report Sections

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