FlyBase Allele Report: Dmel\sgl[08310]

General Information

Symbol

Dmel\sgl⁰⁸³¹⁰

Species

D. melanogaster

Name

FlyBase ID

FBal0009608

Feature type

allele

Associated gene

Dmel\sgl

Associated Insertion(s)

P{PZ}sgl⁰⁸³¹⁰

Carried in Construct

Also Known As

l(3)08310, sgl^l(3)08310, sgl^P1731

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Binari et al., 1997)

Mutagen

P-element activity

(Binari et al., 1997, Hacker et al., 1997, Haerry et al., 1997)

Progenitor genotype

Associated Insertion(s)

P{PZ}sgl⁰⁸³¹⁰

Cytology

Description

Insertion of a P{PZ} element into the 5' untranslated region.

(Binari et al., 1997)

Insertion of a P{PZ} element 106bp upstream of the putative ATG start codon.

(Hacker et al., 1997)

Insertion of a P{PZ} element in the 5' untranslated region.

(Haerry et al., 1997)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:6,964,653..6,964,660 [-]

(Luders et al., 2003, Strigini and Cohen, 2000, Lin et al., 1999, Spradling et al., 1999, Harvie et al., 1998, Binari et al., 1997, Hacker et al., 1997, Haerry et al., 1997, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

cuticle

(Perrimon et al., 1996)

embryo | germline clone

(Hacker et al., 1997)

embryonic/first instar larval cuticle

(Zhu et al., 2005, Binari et al., 1997)

embryonic/first instar larval cuticle | dorsal

(Binari et al., 1997)

embryonic/first instar larval cuticle | germline clone

(Haerry et al., 1997)

embryonic/larval ganglionic tracheal branch

(Lin et al., 1999)

embryonic/larval tracheal dorsal trunk

(Lin et al., 1999)

embryonic/larval tracheal lateral trunk

(Lin et al., 1999)

embryonic/larval tracheal system

(Lin et al., 1999)

embryonic/larval tracheal system | germline clone

(Lin et al., 1999)

embryonic Malpighian tubule | germline clone

(Hacker et al., 1997)

embryonic midgut constriction 2 | germline clone

(Hacker et al., 1997)

mesoderm | germline clone

(Lin et al., 1999)

segment | ventral

(Binari et al., 1997)

stomodeal invagination | germline clone

(Hacker et al., 1997)

ventral denticle belt

(Hacker et al., 1997, Perrimon et al., 1996)

ventral denticle belt | germline clone

(Haerry et al., 1997)

wing | somatic clone

(Binari et al., 1997)

wing margin bristle | somatic clone

(Binari et al., 1997)

Detailed Description

Statement

Reference

When homozygous clones are induced in females using a technique that ensures that all eggs produced by these females are derived from homozygous germline clones and 76% of the follicles also carry homozygous somatic clones, then none of the cuticles of the resulting embryos have a dorsalised phenotype.

(Zhu et al., 2007)

sgl⁰⁸³¹⁰ embryos that lack both maternal and zygotic sgl exhibit a segment polarity phenotype. Salivary glands are present in these mutants.

(Zhu et al., 2005)

When homozygous clones are made in the follicle cells, no dorsalisation phenotypes are seen in the resulting embryos.

(Luders et al., 2003)

Migration of the mesoderm fails to occur properly in homozygous embryos derived from homozygous female germline clones (lacking both maternal and zygotic sgl function). The early steps in tracheal branching are significantly disturbed in stage 13 homozygous embryos (lacking zygotic sgl function). By late stage 15, tracheal branch formation is incomplete, as shown by the presence of large gaps in the dorsal and lateral trunks and stalled ganglionic branches. The penetrance of this phenotype is incomplete and the expressivity is variable; 7% of embryos show some degree of tracheal abnormality, ranging from one to all segments having breaks in the dorsal trunk. Virtually no tracheal branches are seen in homozygous embryos derived from homozygous female germline clones (lacking both maternal and zygotic sgl function).

(Lin et al., 1999)

Embryos derived from homozygous germ line clones in females have a cuticle phenotype identical to wg^- embryos. This phenotype is zygotically (paternally) rescuable, but not to wild-type; zygotically rescued embryos have weak segmental fusions in the ventral part of the embryo and are often missing dorsal cuticle pattern elements. Homozygous clones in the wing result in the loss of anterior margin bristles and wing tissue.

(Binari et al., 1997)

Homozygous embryos derived from heterozygous females die at the third larval instar or early pupal stage. Homozygous embryos derived from females with homozygous sgl⁰⁸³¹⁰ germline clones die as embryos with a severe segment polarity phenotype. These embryos can be partially rescued by a paternal wild-type copy of sgl; 30% of paternally rescued embryos exhibit a weak segment polarity phenotype (partial fusion of the denticle bands) and do not hatch. Homozygous embryos derived from females with homozygous sgl⁰⁸³¹⁰ germline clones have 2-3 invaginations in the dorsal epithelium of the foregut (which will give rise to the stomatogastric nervous system) that are fused at the base. The second midgut constriction is not formed. The Malpighian tubules are very short.

(Hacker et al., 1997)

Hemizygotes die between the first and second larval instar stages. Homozygous embryos derived from homozygous female germ-line clones show a loss of naked cuticle and a mirror-image duplication of denticle belts. This phenotype is fully rescued if the embryos receive a wild-type copy of sgl from their father. Homozygous somatic clones induced in second instar imaginal discs show no mutant phenotype in any part of the wing or other adult tissues.

(Haerry et al., 1997)

Germline clones produce eggs with patterning defects: unrescued embryos exhibit mirror image duplication of denticle belts, rescued embryos are wild type or exhibit partial fusion of denticle belts.

(Perrimon et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

sgl[+]/sgl⁰⁸³¹⁰ is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of Scer\GAL4^P52, Sema1a^UAS.cYa, Sema1a^k13702

(Cho et al., 2012)

sgl[+]/sgl⁰⁸³¹⁰ is an enhancer of visible phenotype of dsh⁴, dsh^w

(Haerry et al., 1997)

Phenotype Manifest In

Suppressed by

Statement

Reference

sgl⁰⁸³¹⁰ has mesoderm phenotype, suppressible by Scer\GAL4^twi.PG/htl^λ.UAS

(Lin et al., 1999)

sgl⁰⁸³¹⁰ has embryonic/larval tracheal system phenotype, suppressible by bnl^UAS.cSa/Scer\GAL4^69B

(Lin et al., 1999)

sgl⁰⁸³¹⁰ has embryonic/first instar larval cuticle phenotype, suppressible by Scer\GAL4^prd.RG1/wg^l-12.UAS

(Binari et al., 1997)

Enhancer of

Statement

Reference

sgl[+]/sgl⁰⁸³¹⁰ is an enhancer of larval posterior commissure | embryonic stage phenotype of Scer\GAL4^P52, Sema1a^UAS.cYa, Sema1a^k13702

(Cho et al., 2012)

sgl[+]/sgl⁰⁸³¹⁰ is an enhancer of adult phenotype of dsh⁴, dsh^w

(Haerry et al., 1997)

Suppressor of

Statement

Reference

sgl⁰⁸³¹⁰ is a suppressor | partially of embryonic/larval tracheal system phenotype of Scer\GAL4^69B, bnl^UAS.cSa

(Lin et al., 1999)

Other

Statement

Reference

dally^ΔP-188, sgl⁰⁸³¹⁰ has embryonic epidermis phenotype

(Tsuda et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

The commissural axon phenotype (failure to cross the midline) seen in embryos expressing Sema-1a^Scer\UAS.cYa under the control of Scer\GAL4^P52 in a Sema-1a^k13702 null background is significantly enhanced if the embryos are also heterozygous for sgl⁰⁸³¹⁰.

(Cho et al., 2012)

The mesoderm migration defects of sgl⁰⁸³¹⁰ embryos lacking both maternal and zygotic sgl function are partially rescued by htl^{Scer\UAS.T:λ\cI-DD} expressed under the control of Scer\GAL4^twi.PG. Some tracheal branching is recovered if bnl^Scer\UAS.cSa is expressed under the control of Scer\GAL4^69B in sgl⁰⁸³¹⁰ embryos lacking both maternal and zygotic sgl function. The tracheal branching defects produced by expression of bnl^Scer\UAS.cSa under the control of Scer\GAL4^69B are weakly blocked if the embryos are homozygous for sgl⁰⁸³¹⁰.

(Lin et al., 1999)

Embryos heterozygous for dally^ΔP-188 derived from mothers heterozygous for sgl⁰⁸³¹⁰ show cuticle defects characteristic of a reduction in wg signalling.

(Tsuda et al., 1999)

sgl⁰⁸³¹⁰ embryos expressing wg^{l-12.Scer\UAS} under the control of Scer\GAL4^prd.RG1 show a minor rescue of the sgl⁰⁸³¹⁰ cuticle phenotype, although naked cuticle is not specified.

(Binari et al., 1997)

Expression of wg^{l-12.Scer\UAS} under the control of Scer\GAL4^prd.RG1 in sgl⁰⁸³¹⁰ embryos does not generate naked cuticle. Expression of wg^Scer\UAS.cKa under the control of Scer\GAL4^prd.RG1 in sgl⁰⁸³¹⁰ embryos induces naked cuticle at 25^oC. Narrow regions of naked cuticle are produced when wg^Scer\UAS.cKa is expressed under the control of Scer\GAL4^prd.RG1 in sgl⁰⁸³¹⁰ embryos at 16^oC. Expression of hh^Scer\UAS.cIa under the control of Scer\GAL4^prd.RG1 in sgl⁰⁸³¹⁰ embryos induces naked cuticle at 16^oC and 25^oC. Expression of arm^{S10.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^prd.RG1 in sgl⁰⁸³¹⁰ embryos induces naked cuticle.

(Hacker et al., 1997)

Dominantly enhances the weak phenotype of dsh⁴/Y flies carrying one copy of dsh^w, increasing the proportion of these flies that have defects in adult structures. sgl⁰⁸³¹⁰ homozygotes that are fully rescued by a single copy of sgl^Ubi-p63E.PH die as second instar larvae if they are also heterozygous for wg^en11.

(Haerry et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

sgl⁰⁵⁰⁰⁷

(BDGP Project Members, 1994-1999)

Rescued by

sgl⁰⁸³¹⁰ is rescued by sgl^Ubi-p63E.PH

(Haerry et al., 1997)

Comments

30% of homozygous embryos derived from females with homozygous sgl⁰⁸³¹⁰ germline clones are rescued by injection of sgl^fl.cHa at the syncytial blastoderm stage.

(Hacker et al., 1997)

Homozygotes are generally completely rescued by sgl^Ubi-p63E.PH, although some sgl^Ubi-p63E.PH lines show partial rescue, producing flies with distal deletions of wing veins.

(Haerry et al., 1997)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11731

P{PZ}sgl⁰⁸³¹⁰ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹

Notes on Origin

Discoverer

A. Spradling.

Comments

Complements: sfl⁰³⁸⁴⁴. Complements: argos⁰⁵⁹⁵⁹. Complements: l(3)L4060^L4060.

(BDGP Project Members, 1994-1999)

Somatic clonal analysis indicates that sgl acts non-cell-autonomously.

(Binari et al., 1997)

External Crossreferences and Linkouts ( 2 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AF009013

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

AAB63462

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

P1731

(Binari et al., 1997)

l(3)08310

(Spradling et al., 1999, Benevolenskaya et al., 1998, Binari et al., 1997, Hacker et al., 1997, Haerry et al., 1997)

l(3)08310⁰⁸³¹⁰

sgl⁰⁸³¹⁰

(Cho et al., 2012, Zhu et al., 2007)

sgl^P1731

(Pfeiffer et al., 2002, Toyoda et al., 2000)

sgl^l(3)08310

(Yan et al., 2010, Lin et al., 1999, Tsuda et al., 1999)

ska^P8310

(Haerry et al., 1997)

sugarless⁰⁸³¹⁰

(Strigini and Cohen, 2000)

Name Synonyms

Secondary FlyBase IDs

References (20)

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