FB2024_03 , released June 25, 2024
Allele: Dmel\sti1
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General Information
Symbol
Dmel\sti1
Species
D. melanogaster
Name
FlyBase ID
FBal0011189
Feature type
allele
Associated gene
Dmel\sti
Associated Insertion(s)
Carried in Construct
Also Known As
dck1
Key Links
Genomic Maps

Allele class

hypomorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - genetic evidence

(D'Avino et al., 2004, Carpenter, 1994)
Mutagen
ethyl methanesulfonate
Progenitor genotype
Cytology
Description

The mutation results in a product lacking the C1 and Citron Homology domains and the last portion of the coiled-coil region.

(D'Avino et al., 2004)

Amino acid replacement: R1093term.

(D'Avino et al., 2004)

Amino acid replacement: ?1093term.

(Naim et al., 2004)

Nucleotide substitution: T4013A.

(Naim et al., 2004)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3L:12,510,777..12,510,777 [-]
Nucleotide change:

C12510777T

Amino acid change:

R1093term | sti-PA; R1097term | sti-PB

Reported amino acid change:

R1093term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(D'Avino et al., 2004)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal cytokinesis

      (Naim et al., 2004)

      abnormal mitotic cell cycle

      (Carpenter, 1994, Gatti and Goldberg, 1991)

      abnormal mitotic cell cycle | larval stage (with sti4)

      (D'Avino et al., 2004)

      abnormal mitotic cell cycle | larval stage (with sti5)

      (D'Avino et al., 2004)

      abnormal size | larval stage (with Df(3L)F10)

      (D'Avino et al., 2004)

      abnormal size | larval stage (with sti3)

      (D'Avino et al., 2004)

      abnormal size | larval stage (with sti4)

      (D'Avino et al., 2004)

      abnormal size | spermatogenesis

      (Naim et al., 2004)

      decreased occurrence of cell division | spermatogenesis

      (Naim et al., 2004)

      lethal - all die before end of larval stage | recessive

      (Gatti and Baker, 1989)

      lethal - all die before end of prepupal stage

      (D'Avino et al., 2004)

      lethal - all die before end of prepupal stage (with Df(3L)F10)

      (D'Avino et al., 2004)

      lethal - all die before end of prepupal stage (with sti2)

      (D'Avino et al., 2004)

      lethal - all die before end of prepupal stage (with sti3)

      (D'Avino et al., 2004)

      lethal - all die before end of prepupal stage (with sti4)

      (D'Avino et al., 2004)

      partially lethal - majority die | adult stage (with sti5)

      (D'Avino et al., 2004)

      partially lethal - majority die | pharate adult stage (with sti5)

      (D'Avino et al., 2004)

      some die during larval stage | recessive

      (Gatti and Baker, 1989)

      some die during prepupal stage | recessive

      (D'Avino et al., 2004)

      some die during prepupal stage | recessive (with Df(3L)F10)

      (D'Avino et al., 2004)

      some die during prepupal stage | recessive (with sti2)

      (D'Avino et al., 2004)

      some die during prepupal stage | recessive (with sti3)

      (D'Avino et al., 2004)

      some die during prepupal stage | recessive (with sti4)

      (D'Avino et al., 2004)
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      sti4/sti1 mutant third instar larval brains do not exhibit a mitotic index higher than in wild-type. However, they exhibit a much higher level of polyploid neuroblasts. Approximately 24.2% of sti4/sti1 cells are 4N, 20.9% 8N, and 16.5% over 8N. Approximately 3.3% of sti4/sti1 cells exhibit a hypercondensed phenotype. sti1 homozygous, sti1/sti3, sti1/sti4, and sti1/Df(3L)F10 larvae have very small imaginal discs and are lethal at the prepupal stage. sti5/sti1 mutant third instar larval brains do not exhibit a mitotic index higher than in wild-type. However, they exhibit a much higher level of polyploid neuroblasts. Approximately 41.9% of sti5/sti1 cells are 4N, 19.3% 8N, and 3.2% over 8N. sti5/sti1 third instar neuroblasts do not exhibit a hypercondensed phenotype.

      (D'Avino et al., 2004)

      sti1 brains have high proportions of polyploid cells. However, the chromosomes of these cells are morphologically normal and the mitotic index is similar to wild type. sti1 males have a proliferation defect in the germ cells, resulting in tiny testes that have virtually no dividing spermatocytes.

      (Naim et al., 2004)

      In trans to a deficiency, mitotic chromosomes (both sisters and heterologues) stuck together and show metaphase delay; "successful" anaphase fragments the chromosomes.

      (Carpenter, 1994)

      Imaginal discs of homozygous larvae are small.

      (Gatti and Baker, 1989)

      imaginal discs small 77% highly polyploid cells; no mitotic block

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer

      Shearn and Garen.

      Comments
      Comments

      Based on lethal phase analysis the following sti alleles can be ranked from the most severe to the weakest as follows: sti3 > sti1 = sti2 = sti4 > sti5.

      (D'Avino et al., 2004)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      References (9)