FB2024_03 , released June 25, 2024
Allele: Dmel\Klp3Amei-352
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General Information
Symbol
Dmel\Klp3Amei-352
Species
D. melanogaster
Name
FlyBase ID
FBal0012149
Feature type
allele
Associated gene
Dmel\Klp3A
Associated Insertion(s)
Carried in Construct
Also Known As
mei-352
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
Progenitor genotype
Cytology
Description

Amino acid replacement: E321K.

(Page and Hawley, 2005)

Amino acid replacement: E?K.

(Page et al., 2004)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
X:2,602,494..2,602,494 [-]
Nucleotide change:

G2602494A

Amino acid change:

E321K | Klp3A-PA; E321K | Klp3A-PB

Reported amino acid change:

E321K

Comment:

Nucleotide substitution thought to be responsible for mutant phenotype. Other differences thought to be polymorphic variants.

(Page and Hawley, 2005)
point_mutation
X:2,601,629..2,601,629 [-]
Nucleotide change:

T2601629C

Amino acid change:

I609T | Klp3A-PA; I609T | Klp3A-PB

Reported amino acid change:

I609T

Comment:

Likely to be polymorphism. Other observed change in mutant thought to be responsible for mutant phenotype.

(Page and Hawley, 2005)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal meiotic cell cycle

      (Page and Hawley, 2005)

      abnormal meiotic cell cycle (with Df(1)ED6579)

      (Page and Hawley, 2005)

      abnormal meiotic cell cycle | recessive

      (Baker and Carpenter, 1972)

      female semi-sterile

      (Baker and Carpenter, 1972)

      female semi-sterile (with Klp3A2)

      (Page and Hawley, 2005)

      female semi-sterile (with Klp3A63e4)

      (Page and Hawley, 2005)

      female sterile

      (Page and Hawley, 2005)

      female sterile (with Df(1)ED6579)

      (Page and Hawley, 2005)

      female sterile (with Klp3A1)

      (Page and Hawley, 2005)

      female sterile (with Klp3A6)

      (Page and Hawley, 2005)

      lethal - all die during embryonic stage | maternal effect

      (Page and Hawley, 2005)

      lethal - all die during embryonic stage | maternal effect (with Df(1)ED6579)

      (Page and Hawley, 2005)

      lethal - all die during embryonic stage | maternal effect (with Klp3A1)

      (Page and Hawley, 2005)

      lethal - all die during embryonic stage | maternal effect (with Klp3A2)

      (Page and Hawley, 2005)

      lethal - all die during embryonic stage | maternal effect (with Klp3A6)

      (Page and Hawley, 2005)

      lethal - all die during embryonic stage | maternal effect (with Klp3A63e4)

      (Page and Hawley, 2005)

      some die during embryonic stage | maternal effect

      (Page and Hawley, 2005)

      some die during embryonic stage | maternal effect (with Df(1)ED6579)

      (Page and Hawley, 2005)

      some die during embryonic stage | maternal effect (with Klp3A1)

      (Page and Hawley, 2005)

      some die during embryonic stage | maternal effect (with Klp3A2)

      (Page and Hawley, 2005)

      some die during embryonic stage | maternal effect (with Klp3A6)

      (Page and Hawley, 2005)

      some die during embryonic stage | maternal effect (with Klp3A63e4)

      (Page and Hawley, 2005)
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The total meiotic exchange frequency for chromosome arm 2L shows little deviation from that of wild-type for homozygous and Klp3Amei-352/Df(1)ED6579 females, but the distribution of exchanges along the chromosome arm is altered. Klp3Amei-352 shows reduced female fertility both as a homozygote (3.7% of eggs hatch) and in transheterozygous combination with other Klp3A mutants (% eggs hatching given in each case); Df(1)ED6579 (2.02%), Klp3A1 (1.90%), Klp3A6 (1.46%) and Klp3A2 (10.29%). The majority of embryos derived from homozygous, Klp3Amei-352/Klp3A2, Klp3Amei-352/Klp3A1 or Klp3Amei-352/Klp3A6 females appear to arrest very early, with only one small nucleus plus a mass of polar body chromatin.

      (Page and Hawley, 2005)

      Homozygous larval brain ganglia have a reduced ability to synthesise DNA after exposure to N-acetoxy-2-acetylaminofluorene compared to wild-type.

      (Boyd and Shaw, 1982)

      Elevates meiotic exchange in the proximal euchromatin of the X chromosome.

      (Carpenter and Baker, 1982)

      Hemizygous male larvae are not sensitive to HN2 and MMS.

      (Mason et al., 1981)

      Mutants are weakly recombination defective. Mutants do not show sensitivity to UV light, γ rays or X rays. Homozygotes and hemizygotes do not show an increased frequency of spontaneous chromosome aberrations in neuroblast metaphases compared to wild-type larvae.

      (Gatti, 1979)

      Klp3Amei-352 mutation increases mitotic chromosome instability approximately three-fold.

      (Baker et al., 1978)

      Klp3Amei-352 females show exchange between the normally achiasmate fourth chromosomes.

      (Sandler and Szauter, 1978)

      Homozygous females are more sterile than can be accounted for by the observed frequencies of aneuploid ova. <up>FlyBase curator comment: FBrf0187654 demonstrates that the semisterility of mutant females is due to maternal-effect lethality, and that both the reduced fertility and the exchange phenotype are the consequence of the same mutational lesion.</up>

      (Baker and Carpenter, 1972)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer

      FBrf0023919 noted that homozygous females have a more severe defect in fertility than would be predicted given the frequency of nondisjunction seen among surviving progeny and did not determine whether the meiotic and semisterility phenotypes were due to a single mutation or to mutations in two separate loci on the X chromosome. FBrf0187654 demonstrates that the semisterility is a consequence of maternal effect lethality an that both the meiotic and semisterility phenotypes of Klp3Amei-352 mutants are due to a single lesion, in the Klp3A locus.

      (Page and Hawley, 2005)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Klp3Amei-352
      mei-352
      (Boyd and Shaw, 1982, Carpenter and Baker, 1982, Mason et al., 1981, Gatti, 1979)
      mei-3521
      (Page and Hawley, 2005)
      Name Synonyms
      Secondary FlyBase IDs
        References (10)