FB2024_03 , released June 25, 2024
Allele: Dmel\mle4
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General Information
Symbol
Dmel\mle4
Species
D. melanogaster
Name
FlyBase ID
FBal0012337
Feature type
allele
Associated gene
Dmel\mle
Associated Insertion(s)
Carried in Construct
Also Known As
mlets
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
Progenitor genotype
Cytology
Description

Amino acid replacement: T415S.

(Richter et al., 1996)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:5,978,347..5,978,347 [-]
Nucleotide change:

A5978347T

Amino acid change:

T415S | mle-PA; T58S | mle-PC

Reported amino acid change:

T415S

Comment:

Amino acid replacement reported as reported as Thr to Ser, which could result from a nucleotide substitution in the first or second base of the codon. The mutation was annotated at the first base of the codon.

(Richter et al., 1996)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Males arise at a low frequency when raised at a permissive temperature. These rare escapers are sterile and produce non-motile sperm.

      (Rastelli and Kuroda, 1998)

      Homozygous adult males escape lethality at a rate of 1/150 at 18oC. In prunspecified males, gl60j males and pp males that are also mutant for mle4, the eyes are darker than for their mle4/+ sibs. rsP1 and Nspl-1 males that are also homozygous for mle4 show a less severe phenotype than their mle4/+ sibs. vg79d5 males that are also mutant for mle4 show less severe phenotype than females also mutant for mle4. mle4 has very little effect on the dosage compensation of wa : homozygous males have slightly darker eyes than their heterozygous brothers. wAdh.PB males that are also mutant for mle4 show paler eye pigmentation with respect their mle4/+ sibs. KrIf-1 and B1 males that are also mutant for mle4 show a more severe phenotype than their mle4/+ sibs.

      (Hiebert and Birchler, 1994)

      Homozygous males show abnormal dosage compensation as determined by tritiated uridine labeling of polytene chromosomes.

      (Okuno et al., 1984)

      male-lethal temperature-sensitive male-lethal 18oC survivors sterile, small and slow to develop

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Occasionally females heterozygous for Sxlf2 and homozygous for mle4 (at 18oC) have sex combs on the basitarsi of their forelegs. Some females homozygous for mle4 and heterozygous for Sxlf1 have sex combs on the basitarsi of their forelegs at 25oC (restrictive temperature). This phenotype occurs with a higher frequency in females with homozygous mle4 mothers. Intersexes are seen in females which are homozygous for mle4 and either carrying one functional copy of Sxl or transheterozygous for Sxlf1 and Sxlfhv1.

      (Skripsky and Lucchesi, 1982)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Belote, 1977.

      Comments
      Comments

      mle4 has no effect on the dosage compensation of we, wapl, wF4-2, wa2, Dp(1;1)w+61e19 or wi.

      (Hiebert and Birchler, 1994)

      Single amino acid change is not responsible for the mutant phenotype: transgenic copy (P{HA-ts}) binds to the X chromosome and fails to complement mle mutants.

      (Richter et al., 1996)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      mle4
      mlets
      (Rastelli and Kuroda, 1998, Richter et al., 1996, Okuno et al., 1984, Skripsky and Lucchesi, 1982)
      Name Synonyms
      Secondary FlyBase IDs
        References (9)