FlyBase Allele Report: Dmel\mys[ts1]

General Information

Symbol

Dmel\mys^ts1

Species

D. melanogaster

Name

FlyBase ID

FBal0012691

Feature type

allele

Associated gene

Dmel\mys

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

heat sensitive hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

heat sensitive hypomorphic allele - genetic evidence

(Bunch et al., 1992)

Mutagen

ethyl methanesulfonate

Progenitor genotype

Cytology

Description

May contain regulatory mutation as lesion not found within exons or splice sites of mys.

(Jannuzi et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

bouton

(Beumer et al., 2002)

bouton & neuromuscular junction

(Beumer et al., 1999)

bouton & neuromuscular junction & abdominal lateral longitudinal muscle 1

(Beumer et al., 1999)

bouton & neuromuscular junction & abdominal ventral longitudinal muscle 1

(Beumer et al., 1999)

bouton & neuromuscular junction & abdominal ventral longitudinal muscle 3

(Beumer et al., 1999)

germinal proliferation center hub | heat sensitive

(Lee et al., 2016)

neuromuscular junction

(Beumer et al., 2002, Beumer et al., 1999)

neuromuscular junction & abdominal lateral longitudinal muscle 1

(Beumer et al., 1999)

neuromuscular junction & abdominal ventral longitudinal muscle 1

(Beumer et al., 1999)

NMJ bouton | third instar larval stage

(Lee et al., 2017)

Detailed Description

Statement

Reference

mys^ts1 mutant third instar larvae show a synapse overgrowth phenotype with an increase in the number of synaptic boutons as well as immature undifferentiated 'ghost boutons' on the neuromuscular junctions. Unlike in wild-type, high K[+] stimulation in mys^ts1 mutants fails to induce a significant further increase in the number of ghost boutons and neither does it increase the bouton size. Similarly, the bouton size is also not increased upon high frequency (10Hz) nerve stimulation.

(Lee et al., 2017)

mys^ts1/mys^ts1 testes (at 25[o]C) show hub mislocalization (significant increase in distance from the tip to the hub compared to controls), though overall hub structure is not altered; at 29[o]C mys^ts1/mys^ts1 is semi-lethal but surviving mutant testes display similar 'pulling' hub phenotypes as seen in shv¹ mutants.

(Lee et al., 2016)

Normal transverse tubule formation is seen in the abdominal temporary eclosion muscles of mys¹/mys^ts1 pharate adults.

(Ribeiro et al., 2011)

Homozygous and mys^ts1/mys⁸ flies show normal odour avoidance behaviour.

(Bhandari et al., 2006)

Mutant synapses at the larval neuromuscular junction show structural overgrowth, with an increased number of boutons in a highly branched, over-elaborated synaptic arbor.

(Beumer et al., 2002)

Neuromuscular junctions (NMJs) of homozygous larvae are significantly larger and more complex than normal. This overgrowth phenotype is similar in animals raised at 25^oC and 29^oC, and is more obvious at the normally simpler muscle 4 terminal than the more complex muscle 12 terminal. The number of primary NMJ branches on muscle 4 is normal, but all higher order branching is very significantly increased, resulting in a highly significant increase in total branching. The number of type I boutons is significantly increased in the NMJs. The neuromuscular junctions of mys^ts1/mys¹¹ larvae show no significant increase in bouton numbers. Homozygous and mys^ts1/mys¹¹ larvae show altered synapse specificity at the muscle 12 NMJ. The NMJs may show a "backbranched" phenotype; at least one arboreal branch leaves muscle 12 and branches back onto muscle 13 from a site away from the point of nerve entry onto muscle 12. The NMJs may show a "pulled away" phenotype; boutons are suspended in the space between muscles 12 and 13 and defasciculation, or branching, occurs before the nerve has actually reached the point of insertion on the muscle. The NMJs may show a "multiple insertions" phenotype, where two or more separate and distinct nerve entry points can be seen on muscle 12. In these junctions, both nerves can frequently be traced back to a point of separation above muscle 13. Homozygous and mys^ts1/mys¹¹ larvae have significantly larger nerve-evoked excitatory junctional current (EJC) amplitudes than normal at the muscle 4 NMJ. The frequency and mean (quantal) amplitude of miniature EJCs is normal at the NMJ in homozygous larvae.

(Beumer et al., 1999)

Lethality occurs during the embryonic/larval stages.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

mys^ts1 has abnormal neuroanatomy | third instar larval stage phenotype, non-enhanceable by shv¹/shv¹

(Lee et al., 2017)

Suppressed by

Statement

Reference

mys^ts1 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by shv^UAS.Tag:HA/Scer\GAL4^nSyb.PS

(Lee et al., 2017)

mys^ts1 has abnormal neuroanatomy phenotype, suppressible by Scer\GAL4^e22c/CaMKII^UAS.cKa

(Beumer et al., 2002)

mys^ts1 has abnormal neuroanatomy phenotype, suppressible by Fas2^e86

(Beumer et al., 2002)

mys^ts1 has abnormal neuroanatomy phenotype, suppressible by Scer\GAL4^Mhc.PW/CaMKII^UAS.cKa

(Beumer et al., 2002)

NOT suppressed by

Statement

Reference

mys^ts1 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Scer\GAL4^how-24B/shv^UAS.Tag:HA

(Lee et al., 2017)

mys^ts1 has abnormal neuroanatomy phenotype, non-suppressible by Scer\GAL4^elav-C155/CaMKII^UAS.cKa

(Beumer et al., 2002)

NOT Enhancer of

Statement

Reference

mys^ts1/mys^ts1 is a non-enhancer of abnormal neuroanatomy | third instar larval stage phenotype of shv¹

(Lee et al., 2017)

Suppressor of

Statement

Reference

mys^ts1 is a suppressor of abnormal neuroanatomy phenotype of Fas2^e86

(Beumer et al., 2002)

Other

Statement

Reference

mys[+]/mys^ts1, tnc^EY03355 has abnormal neurophysiology | larval stage phenotype

(Wang et al., 2018)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

mys^ts1 has NMJ bouton | third instar larval stage phenotype, non-enhanceable by shv¹/shv¹

(Lee et al., 2017)

mys^ts1 has germinal proliferation center hub | heat sensitive phenotype, non-enhanceable by shv¹/shv¹

(Lee et al., 2016)

Suppressed by

Statement

Reference

mys^ts1 has NMJ bouton | third instar larval stage phenotype, suppressible by shv^UAS.Tag:HA/Scer\GAL4^nSyb.PS

(Lee et al., 2017)

mys^ts1 has bouton phenotype, suppressible by Scer\GAL4^e22c/CaMKII^UAS.cKa

(Beumer et al., 2002)

mys^ts1 has bouton phenotype, suppressible by Fas2^e86

(Beumer et al., 2002)

mys^ts1 has neuromuscular junction phenotype, suppressible by Scer\GAL4^e22c/CaMKII^UAS.cKa

(Beumer et al., 2002)

mys^ts1 has neuromuscular junction phenotype, suppressible by Fas2^e86

(Beumer et al., 2002)

NOT suppressed by

Statement

Reference

mys^ts1 has NMJ bouton | third instar larval stage phenotype, non-suppressible by Scer\GAL4^how-24B/shv^UAS.Tag:HA

(Lee et al., 2017)

mys^ts1 has neuromuscular junction phenotype, non-suppressible by Scer\GAL4^elav-C155/CaMKII^UAS.cKa

(Beumer et al., 2002)

NOT Enhancer of

Statement

Reference

mys^ts1/mys^ts1 is a non-enhancer of NMJ bouton | third instar larval stage phenotype of shv¹

(Lee et al., 2017)

mys^ts1/mys^ts1 is a non-enhancer of germinal proliferation center hub phenotype of shv¹

(Lee et al., 2016)

Suppressor of

Statement

Reference

mys^ts1 is a suppressor of bouton phenotype of Fas2^e86

(Beumer et al., 2002)

mys^ts1 is a suppressor of neuromuscular junction phenotype of Fas2^e86

(Beumer et al., 2002)

Other

Statement

Reference

mys[+]/mys^ts1, tnc^EY03355 has embryonic/larval neuromuscular junction | larval stage phenotype

(Wang et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Neurotransmission across the muscle 6 neuromuscular junctions in tnc^EY03355, mys^ts1 double heterozygotes displays significant decreases in mEJP frequency and amplitude, as compared to either heterozygous controls.

(Wang et al., 2018)

shv¹;mys^ts1 double mutant third instar larvae display a synaptic overgrowth phenotype (increased number of boutons and immature, undifferentiated 'ghost boutons') that is comparable in severity to those observed in each of the mutants alone.

Expression of shv^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^nSyb.PS (but not Scer\GAL4^how-24B) rescues the increased number of boutons on neuromuscular junctions characteristic for mys^ts1 mutant third instar larvae.

(Lee et al., 2017)

mys^ts1/mys^ts1; shv¹/+ or mys^ts1/mys^ts1; shv¹/shv¹ testes (at 29[o]C) show similar hub mislocalization phenotypes (increased distance from tip to hub compared to controls) as either single mys^ts1/mys^ts1 or shv¹/shv¹ mutant alone.

(Lee et al., 2016)

Animals carrying Rat\CamKII-I^Ala.hs which are also mutant for mys^ts1 show an approximately 16% increase in bouton number at the neuromuscular junction. Expression of CaMKII^Scer\UAS.cKa under the control of Scer\GAL4^elav-C155 has no effect on the overgrowth phenotype seen at the mys^ts1 larval neuromuscular junction. Expression of CaMKII^Scer\UAS.cKa under the control of Scer\GAL4^Mhc.PW results in a slight, but insignificant, decrease in the overgrowth phenotype seen at the mys^ts1 larval neuromuscular junction, partially rescuing the overproduction of type I boutons. Expression of CaMKII^Scer\UAS.cKa under the control of Scer\GAL4^e22c completely rescues the overgrowth phenotype seen at the mys^ts1 larval neuromuscular junction. Bouton numbers at the larval neuromuscular junction are not significantly different from wild type in Fas2^e86 mys^ts1 double mutants.

(Beumer et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

mys¹¹

(Bunch et al., 1992)

mys¹⁰

(Bunch et al., 1992)

mys¹

(Bunch et al., 1992)

mys^xR04

(Bunch et al., 1992)

mys^XN101

(Bunch et al., 1992)

Rescued by

mys^ts1 is rescued by mys^UAS.cUa/Scer\GAL4^C833

(Lee et al., 2016)

Not rescued by

mys^ts1 is not rescued by mys^UAS.cDa/Scer\GAL4^Mhc.PW

(Beumer et al., 2002)

Comments

Expression of mys^Scer\UAS.cUa driven by Scer\GAL4^C833 rescues hub mislocalization in mys^ts1/mys^ts1 testes.

(Lee et al., 2016)

Fails to complement mys¹⁰, mys^XN101, mys^xR04, mys¹ and mys¹¹ at elevated temperatures.

(Bunch et al., 1992)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

3169

cm¹ ct⁶ mys^ts1

Kyoto

106890

cm¹ ct⁶ mys^ts1

Notes on Origin

Discoverer

Wright.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

l(1)mys^ts

(Deak et al., 1982)

mys^ts1

(Kim et al., 2018, Wang et al., 2018, Lee et al., 2017, Lee et al., 2016, Ribeiro et al., 2011, Rushton et al., 2009, Bhandari et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (15)

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