FB2024_03 , released June 25, 2024
Allele: Dmel\ndl1
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General Information
Symbol
Dmel\ndl1
Species
D. melanogaster
Name
FlyBase ID
FBal0012921
Feature type
allele
Associated gene
Dmel\ndl
Associated Insertion(s)
Carried in Construct
Also Known As
ndl046, ndl46
Key Links
Genomic Maps

Allele class

hypomorphic allele - genetic evidence

hypomorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - genetic evidence

(LeMosy and Hashimoto, 2000)

hypomorphic allele - molecular evidence

(LeMosy et al., 2000)
Mutagen
ethyl methanesulfonate
(Hong and Hashimoto, 1996, Tearle and Nusslein-Volhard, 1987)
Progenitor genotype
Cytology
Description

Nucleotide substitution: G4100A.

(LeMosy et al., 2000)

The protease domain of this allele has been sequenced. Amino acid replacement: V1278M. Nucleotide substitution: a G to A transition.

(Hong and Hashimoto, 1996)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3L:6,598,612..6,598,612 [-]
Nucleotide change:

G6598612A

Reported nucleotide change:

G?A

Amino acid change:

V1278M | ndl-PA

Reported amino acid change:

V1278M

Comment:

Position of mutation on reference sequence inferred by FlyBase curator based on author statement.

(LeMosy et al., 2000, Hong and Hashimoto, 1996)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      In eggs from mutant mothers, the vitelline membrane is permeable to Neutral red dye.

      (LeMosy and Hashimoto, 2000)

      Allele has partial activity in dorsoventral polarity establishment.

      (LeMosy et al., 2000)

      Class II allele. Affected embryos show temperature sensitive dorsalization. The degree of dorsalization varies from so mild as to allow hatching (8% at 18oC) to completely dorsalized (66% at 18oC). Eggs are not fragile.

      (Hong and Hashimoto, 1996)

      Homozygous females produce dorsalised embryos that are unusually fragile as they are contained within the vitelline envelope not the entire eggshell. The cuticle is twisted and lacks ventral and lateral structures. The percentage of eggs laid by ndl9/ndl1 females that hatch following temperature shifts demonstrate the temperature sensitive period for ndl begins around stage 7 of oogenesis and ends roughly 2 hours after fertilisation.

      (Hong and Hashimoto, 1995)

      Embryos transplanted with perivitelline fluid from Tl2 embryos exhibit a polar gastrulation. At the end of embryogenesis an almost complete restoration of pattern along the dorsoventral axis is seen. The point of injection determines the ventralmost point of the restored pattern.

      (Stein et al., 1991)

      Embryos derived from homozygous females are dorsalised.

      (Carroll et al., 1987)

      maternal-effect lethal embryos. Spacing of transverse stripes of ftz protein altered in mutant embryos (Carroll, Winslow, Twombly, and Scott, 1987). totally dorsalized

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      When pipST2.Scer\UAS is expressed under the control of Scer\GAL4ET3 in ndl1/ndl2 females, the resulting embryos are dorsalised.

      (Sen et al., 2000)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Complements

      ndlLP-2

      (LeMosy et al., 2000)
      Partially complements

      ndl9

      (LeMosy and Hashimoto, 2000)
      Fails to complement

      ndl3

      (LeMosy and Hashimoto, 2000)
      Rescued by

      ndl1 is rescued by ndlGAG.S-A

      (Turcotte and Hashimoto, 2002)
      Comments

      ndl9/ndl1 embryos exhibit a weaker dorsalisation phenotype and vitelline membrane permeability phenotype than either ndl9 or ndl1 over a deficiency. ndl1/ndl3 are as severely defective in both dorsal ventral patterning and eggshell integrity as ndl3 itself.

      (LeMosy and Hashimoto, 2000)

      80% of embryos derived from ndl1/ndlLP-2 or ndl9/ndlLP-2 females hatch at 22oC.

      (LeMosy et al., 2000)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Class II allele.

      (LeMosy et al., 2000)

      Complements most Class I ndl alleles, and the Class II ndl9 allele.

      (Hong and Hashimoto, 1996)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      References (15)