FB2024_03 , released June 25, 2024
Allele: Dmel\okrRU
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General Information
Symbol
Dmel\okrRU
Species
D. melanogaster
Name
FlyBase ID
FBal0013236
Feature type
allele
Associated gene
Dmel\okr
Associated Insertion(s)
Carried in Construct
Also Known As
okraRU, okrRU47, RU, rad54RU
Key Links
Genomic Maps

Allele class

antimorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

antimorphic allele - genetic evidence

(Ghabrial et al., 1998)
Mutagen
ethyl methanesulfonate
(Schupbach and Wieschaus, 1991)
Progenitor genotype
Cytology
Description

Amino acid replacement: Q391term.

(Ghabrial et al., 1998)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2L:2,991,482..2,991,482 [-]
Nucleotide change:

C2991482T

Amino acid change:

Q391term | okr-PA

Reported amino acid change:

Q391term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Ghabrial et al., 1998)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  Bloom syndrome
      modeled by BlmD2, BlmN1
      (Lafave et al., 2014)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      okrAA/okrRU mutant females contain an increased number of meiotically-induced double strand breaks (DSBs) compared to controls.

      Many okrAA/okrRU mutant dorsal appendages are abnormal compared to controls. Instead of spherical shaped karyosomes as is seen in wild-type stage 6 egg chambers, the karyosomes of okrAA/okrRU oocytes are fragmented.

      (Lake et al., 2011)

      okrAA/okrRU larvae are highly sensitive to treatment with X-rays or with methyl methanesulfonate.

      okrAA/okrRU mutants show significant decreases in HR-h (homologous recombination using the homologous chromosome) repair compared to controls in a "Repair Reporter 3" assay when the HR-h pathway is available. There is a significant increase in single-strand annealing and in flanking deletions in the mutant flies compared to controls under these conditions.

      In a "Repair Reporter 3" assay when the HR-h pathway is unavailable, okrRU mutants show significant changes in single-strand annealing (SSA) and non-homologous end joining (NHEJ) compared to controls; the use of SSA is increased and NHEJ is decreased compared to wild-type levels.

      (Klovstad et al., 2008)

      okrAA/okrRU females produce eggs with ventralised eggshells.

      (Barbosa et al., 2007)

      okrRU/Df(2L)JS17 mutants have a similar frequency of single-strand annealing repair (SSA) compared to controls in a P{wIw.FRT} hemizygous assay to study DNA double-stranded break repair when assayed at 38oC, but show a higher frequency of SSA than controls in the same assay at 32oC.

      okrRU/Df(2L)JS17 mutants have a reduced frequency of interhomolog gene conversion (GC) and a corresponding increase in SSA frequencies compared to controls in a P{wIw.FRT}/P{wIw.FRT.yellow} homozygous assay to study DNA double-stranded break repair when assayed at both 32oC and 38oC.

      okrRU/Df(2L)JS17 mutants have an increased SSA frequency compared to controls in a P{wIw.FRT}/P{wIw.FRT.8z} homozygous assay to study DNA double-stranded break repair when assayed at 38oC.

      (Wei and Rong, 2007)

      Hemizygotes show a reduced survival rate after exposure to X rays or to 0.08% methyl methanesulfonate compared to control animals.

      (Abdu et al., 2003)

      Homozygous and hemizygous okrRU/Df(2L)JS17 females produce eggs with a range of eggshell phenotypes; from resembling wild-type with two normal dorsal appendages, having two dorsal appendages fused at the base, having a single dorsal appendage to having little or no dorsal appendage material. Hemizygotes are viable.

      (Ghabrial et al., 1998)

      Most extreme eggs are spindle shaped without dorsal appendages. Other eggs have fused dorsal appendages or of normal morphology but remain unfertilized.

      (Schupbach and Wieschaus, 1991)

      female-sterile homozygous females lay eggs which are of variable shapes; in the most extreme cases, the eggs are longer than normal, more pointed at the posterior end and lack dorsal appendages, resembling eggs produced by the dominant female-sterile mutation Fs(2)D.

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      okrAA/okrRU has female sterile phenotype, suppressible by vilya826

      (Lake et al., 2015)

      okrAA/okrRU has abnormal meiotic cell cycle phenotype, suppressible by tremf05981

      (Lake et al., 2011)

      okrAA/okrRU has abnormal meiotic cell cycle phenotype, suppressible by tremF9

      (Lake et al., 2011)

      okrAA/okrRU, p53unspecified has female sterile phenotype, suppressible by mei-W681

      (Lu et al., 2010)
      NOT suppressed by
      Enhancer of
      Statement
      Reference

      okrAA/okrRU is an enhancer of visible | maternal effect phenotype of p535A-1-4

      (Chakravarti et al., 2022)

      okrAA/okrRU is an enhancer of visible | maternal effect phenotype of p53B41.5

      (Chakravarti et al., 2022)
      Other
      Phenotype Manifest In
      NOT Enhanced by
      Statement
      Reference

      okrAA/okrRU has karyosome phenotype, non-enhanceable by p53B41.5

      (Chakravarti et al., 2022)
      Suppressed by
      Statement
      Reference

      okrAA/okrRU has karyosome phenotype, suppressible | partially by p535A-1-4

      (Chakravarti et al., 2022)

      okrAA/okrRU has karyosome phenotype, suppressible | partially by p53A2.3

      (Chakravarti et al., 2022)

      okrAA/okrRU has karyosome phenotype, suppressible | partially by tremF9

      (Lake et al., 2011)

      okrAA/okrRU has karyosome phenotype, suppressible | partially by tremf05981

      (Lake et al., 2011)

      okrAA/okrRU has dorsal appendage phenotype, suppressible | partially by tremF9

      (Lake et al., 2011)

      okrAA/okrRU, p53unspecified has nurse cell phenotype, suppressible by mei-W681

      (Lu et al., 2010)

      okrAA/okrRU has egg chorion phenotype, suppressible by mei-W681/mei-W681

      (Barbosa et al., 2007)

      okrAA/okrRU has egg chorion phenotype, suppressible by lokp6/lokp6

      (Abdu et al., 2002)

      okrAA/okrRU has oocyte nucleus phenotype, suppressible by lokp6/lokp6

      (Abdu et al., 2002)

      okrAA/okrRU has egg chorion | maternal effect phenotype, suppressible | maternal effect by mei-W68unspecified/Df(2R)LL5

      (Ghabrial and Schupbach, 1999)

      okrRU has egg chorion | maternal effect phenotype, suppressible by mei-41D11/mei-41D1

      (Ghabrial and Schupbach, 1999)
      NOT suppressed by
      Statement
      Reference

      okrAA/okrRU has karyosome phenotype, non-suppressible by p53B41.5

      (Chakravarti et al., 2022)

      okrAA/okrRU has egg chorion phenotype, non-suppressible by mus304D1

      (Abdu et al., 2002)

      okrAA/okrRU has egg chorion phenotype, non-suppressible by p5311-1B-1

      (Abdu et al., 2002)

      okrAA/okrRU has dorsal appendage phenotype, non-suppressible by p5311-1B-1

      (Abdu et al., 2002)

      okrAA/okrRU has dorsal appendage phenotype, non-suppressible by mus304D1

      (Abdu et al., 2002)
      Enhancer of
      Statement
      Reference

      okrAA/okrRU is an enhancer of egg | maternal effect phenotype of p535A-1-4

      (Chakravarti et al., 2022)

      okrAA/okrRU is an enhancer of egg | maternal effect phenotype of p53B41.5

      (Chakravarti et al., 2022)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      tremf05981 suppresses the accumulation of meiotically-induced double stranded breaks seen in region 3 of the germarium in okrAA/okrRU mutant females.

      tremF9 partially suppresses the accumulation of meiotically-induced double stranded breaks seen in region 3 of the germarium in okrAA/okrRU mutant females.

      The karyosome defects observed in okrAA/okrRU are almost completely suppressed in the background of tremF9. The dorsal appendage defects are also partially suppressed.

      The karyosome defects observed in okrAA/okrRU are almost completely suppressed in the background of tremf05981.

      (Lake et al., 2011)

      The ventralised eggshell phenotype produced by okrAA/okrRU females is suppressed by mei-W681.

      (Barbosa et al., 2007)

      The eggshell patterning defects of eggs derived from okrRU/okrAA females are suppressed if the females also carry mei-W68unspecified/Df(2R)LL5. The eggshell patterning defects of eggs derived from okrRU/okrRU females are suppressed if the females also carry mei-41D1/mei-41D11.

      (Ghabrial and Schupbach, 1999)

      Mutation does not suppress the bicaudal phenotype of oskbcd.3UTR.T:Zzzz\His6.

      (Wilson et al., 1996)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (7)
      References (24)