FlyBase Allele Report: Dmel\p[1]

General Information

Symbol

Dmel\p¹

Species

D. melanogaster

Name

FlyBase ID

FBal0013443

Feature type

allele

Associated gene

Dmel\p

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

spontaneous

Nature of the Allele

Allele class

Mutagen

spontaneous

Progenitor genotype

Cytology

Description

Nucleotides 1946-1948 (ATG), part of codons 649-650 of wild-type p are substituted by TT, resulting in a frameshift. The mutated gene encodes residues 1-648 of p, followed by an unrelated segment of 39 amino acids and an early termination codon.

(Falcon-Perez et al., 2007)

Single base pair deletion within codon Q648, resulting in a frameshift. The resulting truncated protein is predicated to contain 648 amino acids from wild-type p followed by 39 additional amino acids. Also contains a silent base change in the P62 codon and a nucleotide change in the p upstream region (POU sequence CAAAT is changed to CCAAT).

(Syrzycka et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:8,661,624..8,661,624 [-]

Comment:

The deletion of a single base (A) in codon Q648 causes a frameshift and early translation termination 39 aa downstream.

(Syrzycka et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eye color

(Falcon-Perez et al., 2007, Silva and Mensua, 1985)

abnormal eye color (with Df(3R)p25)

(Syrzycka et al., 2007)

chemical sensitive | recessive

(Humphreys et al., 1996)

visible

(Falcon-Perez et al., 2007)

visible | recessive

Phenotype Manifest In

pigment cell

(Falcon-Perez et al., 2007, Silva and Mensua, 1985)

Detailed Description

Statement

Reference

Mutant flies show a significant decrease in ommochrome and drosopterin pigment levels compared to wild type.

(Harris et al., 2011)

Eye colour: abnormal with reduced levels of red and brown pigment (24 and 24% of wild-type levels respectively).

(Falcon-Perez et al., 2007)

p¹/Df(3R)p25 flies show a reduction in pteridine (red) eye pigment levels compared to controls.

(Syrzycka et al., 2007)

Homozygous adults are hypersensitive to paraquat.

(Humphreys et al., 1996)

Eye colour: dull ruby with purplish tone. 40% normal red and 33% normal brown pigment

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

p¹ has visible phenotype, non-suppressible by Scer\GAL4^GMR.PS/prt^UAS.cFa

(Falcon-Perez et al., 2007)

p¹ has abnormal eye color phenotype, non-suppressible by Scer\GAL4^GMR.PS/prt^UAS.cFa

(Falcon-Perez et al., 2007)

Enhancer of

Statement

Reference

p¹ is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, aos^GD16463

(Harris et al., 2011)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

p¹ has pigment cell phenotype, non-suppressible by Scer\GAL4^GMR.PS/prt^UAS.cFa

(Falcon-Perez et al., 2007)

Enhancer of

Statement

Reference

p¹ is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, aos^GD16463

(Harris et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The reduction in drosopterin pigment levels that is seen in p¹ flies is not affected by HPS4^W515X.

The reduction in drosopterin pigment levels seen in flies carrying w^dsRNA.GMR is enhanced manyfold if they are also mutant for p¹.

The mispatterning of the eye caused by expression of aos^GD16463 under the control of Scer\GAL4^GMR.PF is modestly enhanced in a p¹ mutant background.

(Harris et al., 2011)

Expression of CG10251^Scer\UAS.cFa under the control of Scer\GAL4^GMR.PS does not rescue the eye colour phenotype of p¹ flies.

(Falcon-Perez et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

p¹ is rescued by p^UAS.cFa/Scer\GAL4^GMR.PS

(Falcon-Perez et al., 2007)

Comments

Images (2)

Holtzman, S. and Kaufman, T. (2013)

Mutant

Wild-type

Stocks (4)

Bloomington

552

p¹

2366

Df(3R)XTA1, Diap1^th-1 st¹ kni^ri-1 rn^roe-1 p¹/Dp(3;3)M95A⁺13, st¹ e¹

Kyoto

105976

p¹

107287

Df(3R)XTA1, Diap1^th-1 st¹ kni^ri-1 rn^roe-1 p¹/Dp(3;3)M95A⁺13, st¹ e¹

Notes on Origin

Discoverer

Morgan, July 1910.

Comments

The p¹, p^p and p^snb alleles have been found to have identical changes in sequence, despite being reported to have arisen independently. It seems more likely that stock contamination, mislabelling, or loss occurred some time during past handling of stocks, rather than the same lesions having occurred independently three times.

(Syrzycka et al., 2007)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

p¹

(Harris et al., 2011, Falcon-Perez et al., 2007, Syrzycka et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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