The "rad201[1]" chromosome contains a number of lesions in two different genes. These are: F50L in the CG42382 annotation, and K61E, V93A and Y108H in the CG2412 annotation (corresponding to the Rad51C gene). In addition, an opus element is inserted in the Rad51C promoter region approximately 400bp upstream of the start codon. This mutant allele was isolated from a natural population, and thus it is not known which of the lesions are neutral and which cause the mutant phenotype.
The rad2011 allele contains a missense mutation (P95L) in the CG2412 open reading frame. No changes are detected in the CG8016 open reading frame.
rad2011 mutants have a similar frequency of single-strand annealing repair (SSA) compared to controls in a P{wIw.FRT} hemizygous assay to study DNA double-stranded break repair when assayed at 32oC or 38oC.
Mutants show an increased frequency of induced dominant lethal mutations in eggs and egg chamber degeneration after irradiation.
Mutant flies irradiated with γ rays show an increased rate of chromosome aberrations in somatic cells and an increase in the lethality of developing flies.
The frequency of spontaneous recessive lethal mutations in chromosomes 2 and 3 during oogenesis in rad2011 flies is 9.3%, compared with 0.6% for wild-type flies. Irradiation with 1.29 x 10-1 Kl/kg γ rays results in a decrease in the frequency of lethal mutations compared to the spontaneous level, whereas irradiation with 7.74 x 10-2 Kl/kg γ rays does not result in a change in the frequency of mutations.
Larvae are supersensitive to ionizing radiation.
Mutant females irradiated with γ rays show a decrease in fertility due to a delay in the development of oocytes and their resorbtion. Irradiated mutant egg chambers also show a high frequency of induced dominant lethality.
Survival of homozygous larvae more sensitive to exposure to γ radiation than controls; chromosome- and chromatid-aberration induction increased over control but ratio of the two types same as control. X-ray-induced X-chromosome loss in immature oocytes greater in rad201 than in control; mature oocytes show no effect of rad201. Post-treatment hyperthermia enhances the effect of irradiation in mature oocytes and control immature oocytes, but not in immature oocytes of rad201.
Df(2L)D1/+, rad2011 has chemical sensitive | dominant phenotype
