FlyBase Allele Report: Dmel\trh[1]

General Information

Symbol

Dmel\trh¹

Species

D. melanogaster

Name

FlyBase ID

FBal0017036

Feature type

allele

Associated gene

Dmel\trh

Associated Insertion(s)

Carried in Construct

Also Known As

trh^5D55

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Wilk et al., 1996)

Mutagen

ethyl methanesulfonate

(Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Amino acid replacement: I233N.

(Chung et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:371,067..371,067 [-]

Nucleotide change:

T371067A

Reported nucleotide change:

T?A

Amino acid change:

Reported amino acid change:

I233N

(Chung et al., 2011)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

trh¹ mutant embryos do not exhibit tracheal invagination with no local apical constrictions or cell-shape changes occurring, with the epithelium remaining flat.

in trh¹ mutant embryos, there is no specific accumulation of either actin or myosin at the invaginating edge.

(Brodu and Casanova, 2006)

In trh¹ homozygous embryos, salivary duct cells fail to invaginate, instead remaining clustered on the surface of the embryo.

(Haberman et al., 2003)

Homozygous mutant embryos have no tracheal tubules.

(Sedaghat et al., 2002)

Mutants lack tracheal cell identity.

(Wolf and Schuh, 2000)

In homozygous embryos the Malpighian tubules empty into a single large sack that is attached to the hindgut and no ureters are evident (in contrast to wild-type embryos where the two anterior tubules join in a common ureter and the two posterior tubules join in a common ureter, so that two ureters empty into the hindgut). The sack could result from the malformation of either the proximal tubules or the ureters. The more distal segments of the tubules, although tubular, fail to elongate or undergo cell rearrangement, resulting in tubules that are less than 1/2 the length and wider than normal tubules. The hindguts are normal in shape but somewhat shorter than normal.

(Jack and Myette, 1999)

The filzkorper do not elongate and trachea do not form properly. The oesophagus, gastric caecae and Malpighian tubules are unaffected.

(Isaac and Andrew, 1996)

Tracheal structures are not formed in the embryo. Only small invaginations can occasionally be detected. Salivary ducts do not form and the filzkorper are defective.

(Wilk et al., 1996)

Trachea absent. Majority of sensory nerves form normally, though incidence of misrouted axons is significantly increased, elongation of axons is slowed and sensory neurons which fail to send out an axon are frequent. These defects are seen most strongly in trh¹, twi¹ double mutants where neither trachea nor muscles are present.

(Younossi-Hartenstein and Hartenstein, 1993)

embryonic lethal. Tracheae are absent and filzkorper not elongated.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

jing[+]/jing⁰¹⁰⁹⁴, trh¹ has tracheal section phenotype, enhanceable by tgo¹/tgo[+]

(Sedaghat et al., 2002)

trh¹ has tracheal section phenotype, enhanceable by jing[+]/jing⁰¹⁰⁹⁴

(Sedaghat et al., 2002)

jing⁰¹⁰⁹⁴, trh¹/trh[+] has tracheal section phenotype, enhanceable by tgo¹/tgo[+]

(Sedaghat et al., 2002)

NOT Enhancer of

Statement

Reference

trh¹ is a non-enhancer of tracheal transverse connective primordium phenotype of jing³

(Sedaghat et al., 2002)

trh¹ is a non-enhancer of adult visceral tracheal branch phenotype of jing³

(Sedaghat et al., 2002)

trh¹ is a non-enhancer of tracheal dorsal trunk primordium phenotype of jing³

(Sedaghat et al., 2002)

NOT Suppressor of

Statement

Reference

trh¹ is a non-suppressor of tracheal dorsal trunk primordium phenotype of jing³

(Sedaghat et al., 2002)

trh¹ is a non-suppressor of tracheal transverse connective primordium phenotype of jing³

(Sedaghat et al., 2002)

trh¹ is a non-suppressor of adult visceral tracheal branch phenotype of jing³

(Sedaghat et al., 2002)

Other

Statement

Reference

btl^H82Δ3, tgo¹, trh¹ has tracheal section phenotype

(Sedaghat et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

trh¹, jing⁰¹⁰⁹⁴ double heterozygous embryos display a loss of about half of their trachea. When tgo¹ is also added, 69% of embryos display a loss of about half of their trachea. jing⁰¹⁰⁹⁴, tgo¹ double heterozygous embryos display a loss of about 1/5 of their trachea. jing^rev, trh¹ double heterozygotes have a wild-type tracheal system. When btl^H82Δ3 is also added to trh¹, tgo¹ double heterozygous 69% of embryos display a loss of about half of their trachea. The addition of trh¹ to jing³ homozygotes has no effect on the jing³ tracheal phenotype.

(Sedaghat et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

trh alleles fall into an allelic series. From strongest to weakest: trh¹ = trh⁸ > trh⁷ > trh¹⁰⁵¹² > trh⁶ > trh⁵ > trh⁴.

(Isaac and Andrew, 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

trh¹

(Kondo and Hayashi, 2019, Tsikala et al., 2014, Chung et al., 2011, Chung et al., 2009, Inoue and Hayashi, 2007, Brodu and Casanova, 2006, Jiang and Crews, 2003)

trh^5D55

(Ogura et al., 2018, Tajiri et al., 2007, Jin et al., 2001, Wolf and Schuh, 2000, Jones et al., 1998, Kuo et al., 1996, Wilk et al., 1996, Younossi-Hartenstein and Hartenstein, 1993)

trh^5D

(Tearle and Nusslein-Volhard, 1987)

Name Synonyms

Secondary FlyBase IDs

References (21)

Report Sections

Open Close