FlyBase Allele Report: Dmel\N[Ax-71d]

General Information

Symbol

Dmel\N^Ax-71d

Species

D. melanogaster

Name

FlyBase ID

FBal0028450

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

Also Known As

Ax^71d

Key Links

Allele class

antimorphic allele - genetic evidence

Mutagen

X ray

Nature of the Allele

Allele class

antimorphic allele - genetic evidence

(de Celis and Garcia-Bellido, 1994)

Mutagen

X ray

Progenitor genotype

Cytology

Description

Amino acid replacement: S1088I. Mutant partially sequenced; correlated with single amino acid replacements within six adjacent EGF-homologous elements of the N protein; has Gly at residue 2057 characteristic of Oregon R rather than Ser of Canton S (Kelley, Kidd, Deutsch and Young, 1987). Mutation involves a change from serine to isoleucine at residue 1088 in EGF repeat 27; AGT --> ATT (Kelley, Kidd, Deutsch and Young, 1987).

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive | temperature conditional

(Indrasamy et al., 2000)

partially lethal (with N^Ax-1)

(Grushko et al., 1998)

partially lethal (with N^Ax-9)

(Grushko et al., 1998)

viable

(Grushko et al., 1998, Palka et al., 1990)

viable (with N^Ax-16)

(Grushko et al., 1998)

viable (with N^Ax-E2)

(Grushko et al., 1998)

visible | dominant

(Palka et al., 1990)

visible | recessive

(Indrasamy et al., 2000)

Phenotype Manifest In

Detailed Description

Statement

Reference

N^Ax-71d is a temperature-sensitive lethal allele: male viable at 25[o]C but nearly lethal at 29[o]C.

N^Ax-71d mutants exhibit elevated levels of asymmetry relative to Canton-S flies for scutellar and thoracic bristles. These mutants also show reduced mean character size for thoracic bristles.

N^Ax-71d mutants show significantly reduced mean character size for orbital bristles compared to Canton-S flies.

(Indrasamy et al., 2000)

Length of microchaetae, macrochaetae and longitudinal wing veins is reduced with respect to that of wild type. Heteroallelic combinations with lethal Abruptex alleles show stronger phenotypes at 29^oC than when heterozygous with a N null at 29^oC.

(de Celis and Garcia-Bellido, 1994)

Hemizygous males lack the ventral sensillum of wing vein L3 and the anterior cross vein sensillum, and often lack the twin sensilla of the wing margin and the three sensilla of wing vein L3. The marginal nerve often shows neuroma-like swellings and may be truncated. The L3 nerve is often misrouted into posterior regions of the wing which normally do not contain nerves.

(Palka et al., 1990)

Viable, enhancer type.

(Xu et al., 1990)

Homozygous, viable, phenotype like N^Ax-1. Viable with alleles N^Ax-E2 and N^Ax-16 and lethal with N^Ax-1 and N^Ax-9 (negative complementation), but viability restored by Dp(1;2)51b. There is no obvious effect on the Notch phenotype in heterozygotes with Df(1)N-8 or N^55e11, but Ax phenotype is expressed. In heterozygotes with N^Ax-E2, the mutant phenotype is weakly expressed; heterozygotes with lethal alleles N^Ax-59b and N^Ax-59d are lethal. N^Ax-71d is complementary with recessive alleles at Notch at 18^oC, 25^oC and 29^oC. The mutant expression of Ax tends to increase with increasing temperature except that N^Ax-71d/N^nd-2 at 29^oC has no Ax expression.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

N^Ax-71d/N^Ax-1 has partially lethal phenotype, enhanceable by Dp(3;3)MKRS-D2

(Grushko et al., 1998)

N^Ax-71d/N^Ax-1 has partially lethal phenotype, enhanceable by Dp(3;3)bxd¹¹⁰

(Grushko et al., 1998)

N^Ax-9/N^Ax-71d has partially lethal phenotype, enhanceable by Dp(3;3)MKRS-D2

(Grushko et al., 1998)

N^Ax-9/N^Ax-71d has partially lethal phenotype, enhanceable by Dp(3;3)bxd¹¹⁰

(Grushko et al., 1998)

Other

Statement

Reference

Dp(3;3)MKRS-D2, N^Ax-71d has partially lethal phenotype

(Grushko et al., 1998)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Homozygous viability is decreased if the flies also carry Dp(3;3)MKRS-D2.

(Grushko et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Portin & Ruohonen.

Comments

Does not modify the N haplo-insufficient phenotype of loss of the wing margin. N^Ax-71d shows negative complementation with N^Ax-M1.

(de Celis and Garcia-Bellido, 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ax^71d

(Indrasamy et al., 2001, Indrasamy et al., 2000, Grushko et al., 1998, de Celis and Garcia-Bellido, 1994, de Celis et al., 1993, Palka et al., 1990, Xu et al., 1990)

N^Ax-71d

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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