FlyBase Allele Report: Dmel\dpp[e90]

General Information

Symbol

Dmel\dpp^e90

Species

D. melanogaster

Name

FlyBase ID

FBal0031065

Feature type

allele

Associated gene

Dmel\dpp

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Wharton et al., 1996, Wharton et al., 1993)

Progenitor genotype

Cytology

Description

Amino acid replacement: F496I.

(Wharton et al., 1996)

Mutation falls in the C-terminal ligand region of the dpp protein.

(Wharton et al., 1996)

Nucleotide substitution: T2673A.

(Wharton et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:2,458,279..2,458,279 [+]

Nucleotide change:

T2458279A

Reported nucleotide change:

T2673A

Amino acid change:

F496I | dpp-PA; F496I | dpp-PB; F496I | dpp-PC; F496I | dpp-PE

Reported amino acid change:

F496I

(Wharton et al., 1996)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage | heat sensitive

(Wharton et al., 1996)

some die during embryonic stage | heat sensitive

(Wharton et al., 1996)

Phenotype Manifest In

Detailed Description

Statement

Reference

dpp^e90 homozygotes, kept at 18'C until 42 hours APF (equivalent to 20 hours APF at 25'C) and then raised to 25'C for the proceeding 22 hours have slightly abnormal arrangments of inter-ommatidial cells including: 3's which fail to establish a correct position as a vertex of the hexagon; 2'/3's abnormally arranged around sensory bristles; aberrant bristle-bristle contacts.

GA28a. Genetic interaction (class, effect) [CV] *S :

(Cordero et al., 2007)

dpp^e90/dpp^hr56 males raised at 18^oC to eclosion and then shifted to 29^oC, show no significant loss of male germline stem cells after 1 week.

(Kawase et al., 2004)

Females carrying temperature sensitive combinations dpp^e90/dpp^hr56 or dpp^hr4/dpp^hr56 after shifting to the restrictive temperature for one week show 40-50% of germaria that are significantly smaller than wild type. The effect is more pronounced in older females. Stem cell number is reduced, stem cells have differentiated into cysts. Some effect on egg chamber budding is seen.

(Xie and Spradling, 1998)

Embryos show cuticular holes, disorganization of the head skeleton, internalization of the seventh and eighth abdominal segments and the filzkorper. dpp^e90 shows a temperature sensitive embryonic lethality: percentage embryonic lethality drops from 29% at 25^oC to 15% at 18^oC. Temperature sensitivity is evident in denticle pattern as well as survival.

(Wharton et al., 1996)

Homozygous and transheterozygous embryos were examined with respect to 25 cuticular markers, results demonstrate a graded requirement for dpp along the dorso-ventral axis.

(Wharton et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

dpp^e90/dpp[+] is a suppressor | partially of increased cell number | female | adult stage phenotype of Psc^GD4481, Scer\GAL4^C587, Su(z)2^GD16388

(Li et al., 2016)

dpp^e90/dpp[+] is a suppressor | partially of neoplasia | female | adult stage phenotype of Psc^GD4481, Scer\GAL4^C587, Su(z)2^GD16388

(Li et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

dpp^e90 has phenotype, enhanceable by tld¹⁴

(Raftery et al., 1995)

Suppressor of

Statement

Reference

dpp^e90/dpp[+] is a suppressor | partially of germarium phenotype of Psc^GD4481, Scer\GAL4^C587, Su(z)2^GD16388

(Li et al., 2016)

Other

Statement

Reference

dpp^hr56/dpp^e90, gbb^D4 has male germline stem cell phenotype

(Kawase et al., 2004)

dpp^hr56/dpp^e90, gbb[+]/gbb^D4 has male germline stem cell phenotype

(Kawase et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Simultaneous expression of both Psc^GD4481 and Su(z)2^GD16388 under the control of Scer\GAL4^C587 (together with tub-Gal80[ts] to limit the time of expression to adulthood) results in tumorigenic accumulation of spectrosome containing germline stem cell-like (GSC-like) cells (which increase in number with the time of expression) in female germaria; this accumulation of GSC-like cells is significantly suppressed by combination with a single copy of dpp^e90.

(Li et al., 2016)

bam^hs.PO animals cultured at 18^oC and heat shocked as late stage pupae (37^oC, 4 times for 2 hours each with an 8-12 hour interval) produce adult females with agametic germaria. If these animals also carry the temperature sensitive allelic combination dpp^hr56/dpp^e90, and are shifted to 29^oC (the non-permissive temperature) for 10 days, the resulting agametic germaria contain only slightly less follicle cells than controls (bam^hs.PO only).

(Kirilly et al., 2005)

dpp^e90/dpp^hr56; gbb^D4/+ males raised at 18^oC to eclosion and then shifted to 29^oC for one week have an average of 5.6 germ-line stem cells per testis, compared to 8.6 in gbb^D4/+.

(Kawase et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Nusslein-Volhard.

(Wharton et al., 1993)

Comments

Based on considerations of degree of dominant lethality, dpp alleles can be placed in an allelic series. Progressing from weakest to most severe the series is: dpp^e87 < dpp^e90 < dpp^hr56 < dpp^hr4 < dpp^hr92 < dpp^hr27 < dpp^hr93 < dpp^H94 < dpp^H95 < dpp^H96 = dpp^H91 = dpp^H46.

(Wharton et al., 1993)

dpp^e90 shows interallelic complementation at 18^oC with dpp^hr56 and dpp^hr4.

(Wharton et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

dpp⁹⁰

(Iwaki and Lengyel, 2002)

dpp^e90

(Li et al., 2016, Cordero et al., 2007, Kirilly et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (12)

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