FB2024_03 , released June 25, 2024
Allele: Dmel\sr155
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General Information
Symbol
Dmel\sr155
Species
D. melanogaster
Name
FlyBase ID
FBal0032779
Feature type
allele
Associated gene
Dmel\sr
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
    Nature of the Allele
    Allele class

    loss of function allele

    (Usui et al., 2004)
    Mutagen
    Progenitor genotype
    Cytology
    Description

    Single nucleotide substitution resulting in a premature stop codon which corresponds to residue Q590 in the srA isoform and to residue Q316 in the srB isoform.

    (Usui et al., 2004)
    Mutations Mapped to the Genome
    Curation Data
    Type
    Location
    Additional Notes
    References
    point_mutation
    3R:18,130,706..18,130,706 [+]
    Nucleotide change:

    C18130706T

    Reported nucleotide change:

    C198834T

    Amino acid change:

    Q596term | sr-PA; Q316term | sr-PB; Q596term | sr-PD; Q316term | sr-PE

    Reported amino acid change:

    Q590term,Q316term

    (Usui et al., 2004)
    Variant Molecular Consequences
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
        Expression Data
        Reporter Expression
        Additional Information
        Statement
        Reference
         
        Marker for
        Reflects expression of
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Disease
        Interaction
        References
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Disease-implicated variant(s)
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In
        Detailed Description
        Statement
        Reference

        sr155 mutants display tracheal migration phenotypes.

        (Butí et al., 2014)

        ligament attachment cells are missing from the lch5 abdominal lateral pentascolopidial chordotonal organ s of stage 16 sr155/Df(3R)DG4. These organs also occasionally lack one or both cap attachment cells, which when present are deformed.

        (Inbal et al., 2004)

        In sr155/Df(3R)DG4 embryos, stalled migration of the dorsal branch, dorsal trunk and ganglionic branches is seen. In spite of stalled migration, thin cytoplasmic extensions in the correct direction are seen. Defective migration of the visceral branch is also seen. Muscle migration is also abnormal.

        (Dorfman et al., 2002)

        At stage 16 myotubes extend elongated membrane processes that often bifurcate in different directions, often these processes cross the ventral midline. In fully differentiated embryos the myotubes fail to fuse to the epidermis and instead attach to other myotubes.

        (Frommer et al., 1996)

        Double heterozygotes with sr6, sr461, srR947, Df(3R)P14 and sr61j are lethal, double heterozygotes with sr254 and sr410 have muscular defects and double heterozygotes with sr1 and sr5 have muscular and cuticular defects.

        (de la Pompa et al., 1989)
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        Suppressor of
        Statement
        Reference

        sr155/sr[+] is a suppressor of abnormal cell migration phenotype of ptcD130

        (Butí et al., 2014)
        NOT Suppressor of
        Statement
        Reference

        sr155 is a non-suppressor of abnormal cell migration phenotype of ptcD130

        (Butí et al., 2014)
        Other
        Statement
        Reference

        bnl00857, sr155 has abnormal cell migration phenotype

        (Butí et al., 2014)
        Phenotype Manifest In
        Suppressor of
        Statement
        Reference

        sr155/sr[+] is a suppressor of embryonic/larval tracheal system phenotype of ptcD130

        (Butí et al., 2014)

        sr155/sr[+] is a suppressor of embryonic/larval ganglionic tracheal branch phenotype of ptcD130

        (Butí et al., 2014)
        NOT Suppressor of
        Statement
        Reference

        sr155 is a non-suppressor of embryonic/larval tracheal system phenotype of ptcD130

        (Butí et al., 2014)

        sr155 is a non-suppressor of embryonic/larval ganglionic tracheal branch phenotype of ptcD130

        (Butí et al., 2014)
        Other
        Additional Comments
        Genetic Interactions
        Statement
        Reference

        Removing one copy of sr in a ptcD130 background rescues the migration of many ganglionic branches, whereas double mutant embryos exhibit impaired ganglionic branch migration.

        sr155/bnl00857 double mutants exhibit impaired ganglionic branch migration.

        (Butí et al., 2014)
        Xenogenetic Interactions
        Statement
        Reference
        Complementation and Rescue Data
        Comments
        Images (0)
        Mutant
        Wild-type
        Stocks (1)
        Notes on Origin
        Discoverer
        Comments
        Comments

        Strong sr allele.

        (Frommer et al., 1996)

        Severe sr allele.

        (Beiman et al., 1996)
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (1)
        Reported As
        Symbol Synonym
        sr155
        (Greenblatt Ben-El et al., 2017, Nachman et al., 2015, Butí et al., 2014, Subramanian et al., 2007)
        Name Synonyms
        Secondary FlyBase IDs
          References (10)