FlyBase Allele Report: Dmel\N[ΔEGF1-18.hs]

General Information

Symbol

Dmel\N^ΔEGF1-18.hs

Species

D. melanogaster

Name

FlyBase ID

FBal0033800

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

P{ΔEGF1-18}

Key Links

Transgenic product class

inframe_deletion

(Lieber et al., 1993)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Lieber et al., 1993)

Mutagen

in vitro construct

(Lieber et al., 1993)

Progenitor genotype

Carried in construct

P{ΔEGF1-18}

Cytology

Description

Lacks the binding site for the Dl product.

(Ahimou et al., 2004)

Encodes N protein lacking EGF-like repeats 1 to 18.

(Wesley and Saez, 2000)

Deletion within the EGF repeats.

(Lieber et al., 1993)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

short lived | heat sensitive

(Presente et al., 2001)

Phenotype Manifest In

S2 cell-line

(Ahimou et al., 2004)

Detailed Description

Statement

Reference

Cells expressing N^ΔEGF1-18.hs show no significant detachment force from Dl^hs.PL-expressing cells.

Cells expressing N^ΔEGF1-18.hs produce no significant cell aggregates when mixed and rotated with Dl^hs.PL-expressing cells.

The surface topology of cells expressing N^ΔEGF1-18.hs is different from that of control cells.

(Ahimou et al., 2004)

Males carrying N^ΔEGF1-18.hs reared at 25[o]C subjected to two 1 hour heat pulses per day, beginning 3 days post-eclosion, exhibit a significant decline in lifespan. Without the 37[o]C heat pulses, lifespan is unaffected.

(Presente et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

N^ΔEGF1-18.hs fails to rescue N^l1N-ts1

(Presente et al., 2004)

N^ΔEGF1-18.hs fails to rescue N^l1N-ts1

(Presente et al., 2001)

N^ΔEGF1-18.hs fails to rescue N^264-47

(Lieber et al., 1993)

Comments

N^ΔEGF1-18.hs fails to rescue the long term memory defects in N^l1N-ts1 animals.

(Presente et al., 2004)

Unable to rescue the cuticle phenotype of N^264-47 embryos.

(Lieber et al., 1993)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Carried in a plasmid, transfected into S2 cells and used in biochemical assays.

(Wesley, 1999)

Carried in a plasmid and transfected into S2 cells to study the effect of the Dl and wg proteins on N protein.

(Wesley and Saez, 2000)

Stably expressed in S2 cells and used in Notch signaling assays.

(Ahimou et al., 2004)

Incomplete N protein.

(Lieber et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

N^Δ1-18

(Powell et al., 2001)

N^ΔEGF1-18.hs

NΔ1-18

(Ahimou et al., 2004)

S2-N^ΔEGF1-18

(Wesley, 1999)

ΔEGF1-18

(Lieber et al., 1993)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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