FlyBase Allele Report: Dmel\slp1[hs.PC]

General Information

Symbol

Dmel\slp1^hs.PC

Species

D. melanogaster

Name

heat shock construct of Cadigan

FlyBase ID

FBal0035635

Feature type

allele

Associated gene

Dmel\slp1

Associated Insertion(s)

Carried in Construct

P{HS-slp1}

Also Known As

hs-slp1

Key Links

Transgenic product class

wild_type

(Cadigan et al., 1994)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Cadigan et al., 1994)

Mutagen

in vitro construct

(Cadigan et al., 1994)

Progenitor genotype

Carried in construct

P{HS-slp1}

Cytology

Description

Construct: slp1 gene expression is driven by a heat shock promoter.

(Cadigan et al., 1994)

Allele components

Component

Use(s)

Encoded product / tool

slp1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Ectopic expression of slp1^hs.PC using heat shock between 2.5 and 4.0 hours of development (at 22^oC) results in additional GMC-1 -> RP2/sib lineages. Duplication of the RP2/sib lineage is seen in 18% of hemisegments and in 17% of hemisegments triplication of the lineage is seen. Additional neuroblasts, such as NB5-3, NB3-5 and NB5-6 have adopted an NB4-2 identity in these embryos. NB5-3 is the most frequently transformed neuroblast (approximately 35% of hemisegments), with the highest frequency of transformation seen when expression of slp1^hs.PC is induced between 3.0 and 3.5 hours of development.

(Bhat et al., 2000)

Denticle belts of the second, fourth, sixth and eighth abdominal segments are missing.

(Cadigan et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

slp1^hs.PC is a suppressor of RP2sib neuron phenotype of wg^l-17

(Bhat et al., 2000)

slp1^hs.PC is a suppressor of neuroblast NB4-2 phenotype of wg^l-17

(Bhat et al., 2000)

slp1^hs.PC is a suppressor of larval RP2 motor neuron phenotype of wg^l-17

(Bhat et al., 2000)

slp1^hs.PC is a suppressor of RP2sib neuron phenotype of wg^l-8

(Bhat et al., 2000)

slp1^hs.PC is a suppressor of neuroblast NB4-2 phenotype of wg^l-8

(Bhat et al., 2000)

slp1^hs.PC is a suppressor of larval RP2 motor neuron phenotype of wg^l-8

(Bhat et al., 2000)

Other

Statement

Reference

gsb^hs.PBH, slp1^hs.PC has larval RP2 motor neuron phenotype

(Bhat et al., 2000)

gsb^hs.PBH, slp1^hs.PC has RP2sib neuron phenotype

(Bhat et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of slp1^hs.PC in wg^l-8 or wg^l-17 embryos rescues the RP2/sib lineage in as many as 85% of hemisegments. Rescue of NB4-2 is seen in approximately 43% of hemisegments. The RP2/sib lineage is missing in 97% of hemisegments in embryos carrying two copies of gsb^hs.PBH and one copy of slp1^hs.PC, as is seen in embryos carrying only gsb^hs.PBH. In contrast, in embryos containing either one copy of gsb^hs.PBH and one copy of slp1^hs.PC or two copies of gsb^hs.PBH and two copies of slp1^hs.PC, both loss and gain of the RP2/sib lineage is seen in a partially penetrant manner.

(Bhat et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Cadigan.

(Cadigan et al., 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

slp1^hs.PC

Name Synonyms

heat shock construct of Cadigan

Secondary FlyBase IDs

References (4)

Report Sections

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