FlyBase Allele Report: Dmel\orb[F303]

General Information

Symbol

Dmel\orb^F303

Species

D. melanogaster

Name

FlyBase ID

FBal0039707

Feature type

allele

Associated gene

Dmel\orb

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Wong et al., 2011)

Progenitor genotype

Cytology

Description

Amino acid replacement: Y742N.

(Wong et al., 2011)

Nucleotide substitution: T?A.

(Wong et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:23,266,431..23,266,431 [-]

Nucleotide change:

T23266431A

Reported nucleotide change:

T?A

Amino acid change:

Reported amino acid change:

Y742N

(Wong et al., 2011)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dorsal appendage (with orb^mel)

(Mansfield et al., 2002)

embryo (with orb^mel)

(Fang and Lerit, 2022)

oocyte

(Manheim et al., 2002, Chang et al., 1999)

ovary

(Chang et al., 1999)

Detailed Description

Statement

Reference

75% of embryos derived from heterozygous female hatch.

(Castagnetti and Ephrussi, 2003)

16 cell cysts form in mutant ovaries, but the oocyte is abnormal and is located in the centre of the cyst.

(Manheim et al., 2002)

Oogenesis is arrested just after the formation of the 16-cell cyst in mutant ovaries. The presumptive oocyte remains in the centre of the cluster, rather than migrating to the posterior pole.

(Chang et al., 1999)

Ovarian development has arrested at an early stage but a few egg chambers are seen, some have aberrant morphology.

(Lantz et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

orb^F303/orb^mel has lethal | maternal effect | embryonic stage phenotype, suppressible | partially by yps^JM2/yps^JM2

(Mansfield et al., 2002)

NOT suppressed by

Statement

Reference

orb^F303 has female sterile | recessive phenotype, non-suppressible by yps^JM2

(Mansfield et al., 2002)

Phenotype Manifest In

Suppressed by

Statement

Reference

orb^F303/orb^mel has embryo phenotype, suppressible by Plp^WT.Ubi.GFP

(Fang and Lerit, 2022)

orb^F303/orb^mel has oocyte phenotype, suppressible | partially by yps^JM2/yps^JM2

(Martin et al., 2003)

NOT suppressed by

Statement

Reference

orb^F303/orb^mel has dorsal appendage phenotype, non-suppressible by yps^JM2/yps^JM2

(Mansfield et al., 2002)

Other

Statement

Reference

orb^F303/orb^mel, yps^JM2 has egg chamber phenotype

(Mansfield et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

30% of embryos derived from BicC⁵/+ ; orb^F303/+ females hatch. Of the non-hatching embryos, 62% are bicaudal and 30% have head defects.

(Castagnetti and Ephrussi, 2003)

Wild-type patterns of microtubule distribution and cytoplasmic streaming are restored in half of orb^mel/orb^F303 oocytes if they are also homozygous for yps^JM2.

(Martin et al., 2003)

The failure of most eggs derived from orb^mel/orb^F303 females to hatch is partially suppressed by yps^JM2/yps^JM2 (40% of eggs derived from the double mutant females hatch). Eggs laid by yps^JM2 orb^mel/yps^JM2 orb^F303 females have fused, reduced or absent dorsal appendages. 5% of yps^JM2 orb^mel/yps^JM2 orb^F303 egg chambers are bipolar.

(Mansfield et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

58476

w^*; e¹ orb^F303/TM3, Sb¹

Notes on Origin

Discoverer

C. Nusslein-Volhard.

(Lantz et al., 1994)

Comments

Class II orb allele.

(Lantz et al., 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

orb³⁰³

(Cavey et al., 2005)

orb^F303

(Fang and Lerit, 2022, Wong et al., 2011)

orb^f303

(Chang et al., 1999)

Name Synonyms

Secondary FlyBase IDs

FBal0127197

References (14)

Report Sections

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