FB2024_03 , released June 25, 2024
Allele: Ecol\lacZsvp-AE127
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General Information
Symbol
Ecol\lacZsvp-AE127
Species
E. coli
Name
FlyBase ID
FBal0041984
Feature type
allele
Associated gene
Ecol\lacZ
Associated Insertion(s)
P{lwB}svpAE127
Carried in Construct
Also Known As
AE127, svp-lacZ, svpAE127-lacZ
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Zelhof et al., 1997, Treisman and Rubin, 1996, Hoshizaki et al., 1995, Fischer-Vize et al., 1992, Heberlein et al., 1991)
Progenitor genotype
Associated Insertion(s)
P{lwB}svpAE127
Cytology
Description

P-element insertion into the svp gene.

(Heberlein et al., 1991)
Allele components
Component
Use(s)
Inserted element
P{lwB}
Encoded product / tool
lacZ
Tagged with
Tag:NLS(P)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage 11 -- 14
      dorsal vessel primordium
      (Lo and Frasch, 2001)
      embryonic stage 12 -- 15
      embryonic heart cardioblast
      (Lo and Frasch, 2001)
      embryonic stage 15
      embryonic dorsal vessel
      (Lo and Frasch, 2001)
      embryonic/larval corpus allatum
      (Lo and Frasch, 2001)
      embryonic pericardial cell
      (Lo and Frasch, 2001)
      embryonic stage 16 -- 17
      embryonic ostial cardioblast
      (Ponzielli et al., 2002)
      larval stage
      embryonic/larval ostium
      (Ponzielli et al., 2002)
      third instar larval stage
      photoreceptor cell R4
      (Fischer-Vize et al., 1992)
      photoreceptor neuron | presumptive
      (Treisman and Rubin, 1996)
      photoreceptor cell R5
      (Fischer-Vize et al., 1992)
      photoreceptor cell R3
      (Fischer-Vize et al., 1992)
      photoreceptor cell R2
      (Fischer-Vize et al., 1992)
      adult subretinal glial cell | precursor
      (Treisman and Rubin, 1996)
      Additional Information
      Statement
      Reference

      EcollacZsvp-AE127 is expressed in seven segmentally repeated pairs of ostial cardioblasts.

      (Ponzielli et al., 2002)

      Expression is observed in heart progenitors from embryonic stage 11. It is first observed in at least two cells per hemisegment in the posterior half of the embryo. From stage 12, expression observed in 4 strongly-expressing cardioblasts are seen in seven posterior segments in the embryo, with an additional pair of cells situated caudally to these and the pattern is maintained in stages 13-15. In stage 15, strong expression is observed in two adjacent pairs of cardioblasts in seven segments, plus another single pair located immediately poasterior to the last double pair of cardioblasts. The svp pattern is complementary to the tin pattern in that the two pairs of svp-expressing cardioblasts do not express tin. Expression is also observed at the anterior end of the dorsal vessel in cells that will become the corpus allatum and weakly in some pericardial cells.

      (Lo and Frasch, 2001)
       
      Marker for
      embryonic ostial cardioblast
      (Ponzielli et al., 2002)
      photoreceptor cell R2
      (Fischer-Vize et al., 1992)
      photoreceptor cell R3
      (Fischer-Vize et al., 1992)
      photoreceptor cell R4
      (Fischer-Vize et al., 1992)
      photoreceptor cell R5
      (Fischer-Vize et al., 1992)
      Reflects expression of
      svp
      (Ponzielli et al., 2002, Lo and Frasch, 2001, Treisman and Rubin, 1996, Fischer-Vize et al., 1992)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Statement
      Reference

      Ecol\lacZsvp-AE127 is an enhancer of abnormal cell number | embryonic stage phenotype of SB0453

      (Schwarz et al., 2018)
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      Ecol\lacZsvp-AE127 is an enhancer of embryonic cardioblast phenotype of SB0453

      (Schwarz et al., 2018)

      Ecol\lacZsvp-AE127 is an enhancer of heart primordium phenotype of SB0453

      (Schwarz et al., 2018)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      The overall decrease in the number of cardioblasts in SB0453 mutant embryos is not affected by Ecol\lacZsvp-AE127; however, ostial cardioblasts become absent while generic cardioblasts increase in number.

      (Schwarz et al., 2018)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      AE127
      (Lo et al., 2002, Hayashi et al., 1998, Zelhof et al., 1997)
      Ecol\lacZsvp-AE127
      lacZsvp-AE127
      Name Synonyms
      Secondary FlyBase IDs
        References (44)