FlyBase Allele Report: Dmel\rpr[hs.PW]

General Information

Symbol

Dmel\rpr^hs.PW

Species

D. melanogaster

Name

FlyBase ID

FBal0044324

Feature type

allele

Associated gene

Dmel\rpr

Associated Insertion(s)

Carried in Construct

P{hs-rpr}

Also Known As

hs-rpr

Key Links

Transgenic product class

wild_type

(White et al., 1994)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(White et al., 1994)

Mutagen

in vitro construct

(White et al., 1996, Grether et al., 1995, White et al., 1994)

Progenitor genotype

Carried in construct

P{hs-rpr}

Cytology

Description

An Hsp70 promoter drives expression of rpr.

(White et al., 1994)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

rpr

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death

(Kuranaga et al., 2006, Igaki et al., 2002, Lisi et al., 2000, Grether et al., 1995, White et al., 1994)

increased cell death | heat sensitive

(Qi et al., 2004)

lethal

(Lisi et al., 2000)

lethal | dominant | heat sensitive

(White et al., 1996)

Phenotype Manifest In

embryo

(Lisi et al., 2000)

Detailed Description

Statement

Reference

Salivary glands from first, second and early third instar larvae are resistant to expression of rpr^hs.PW, surviving normally and showing no staining for active caspase-3. In contrast, salivary glands from animals after the mid-third instar larval transition are susceptible to cell death induced by rpr^hs.PW.

(Yin et al., 2007)

Expression of rpr^hs.PW in S2 cells leads to increased cell death.

(Kuranaga et al., 2006)

Third larval instar wing discs expressing rpr^hs.PW (using heat shock) show a high level of apoptosis.

(Qi et al., 2004)

Expression of rpr^hs.PW in S2 cells induces cell death.

(Igaki et al., 2002)

Causes apoptosis leading to organismal death in the embryo, when heat shock is applied at 5-10 hours after egg laying.

(Lisi et al., 2000)

Upon heat shock causes massive cell death throughout embryos. This cell death can be also be induced in a Df(3L)H99 background. A single heat pulse at a variety of stages is sufficient to kill flies, though late embryos and late pupae (where the rate of cell division is low) are more resistant to this lethality than other stages. 4-6 hours after a 1 hour heat shock at 39^oC, half the flies carrying the heat inducible rpr died, suddenly.

(White et al., 1996)

Can induce apoptosis in Df(3L)H99 embryos.

(Grether et al., 1995)

Sufficient to induce extensive apoptosis increase in Df(3L)H99 embryos.

(White et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

rpr^hs.PW has increased cell death | heat sensitive phenotype, non-enhanceable by Ada2b^unspecified

(Qi et al., 2004)

Suppressed by

Statement

Reference

rpr^hs.PW has abnormal cell death phenotype, suppressible by Diap1^SL

(Lisi et al., 2000)

rpr^hs.PW has increased cell death phenotype, suppressible by abs^14B

(Irion and Leptin, 1999)

NOT suppressed by

Statement

Reference

rpr^hs.PW has increased cell death | heat sensitive phenotype, non-suppressible by Ada2b²

(Qi et al., 2004)

Other

Statement

Reference

Diap1^SL, rpr^hs.PW has viable phenotype

(Lisi et al., 2000)

Phenotype Manifest In

Suppressed by

Statement

Reference

rpr^hs.PW has embryo phenotype, suppressible by Diap1^SL

(Lisi et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The high level of apoptosis caused by expression of rpr^hs.PW in the third larval instar wing disc is not affected if the discs are also mutant for Ada2b^unspecified.

(Qi et al., 2004)

The cell death induced by rpr^hs.PW in S2 cells is blocked almost completely by treating the cells with Nc dsRNA (produced by annealing Nc^cIa and Nc^a.cIa RNA). The cell death induced by rpr^hs.PW in S2 cells is partly suppressed by treating the cells with Ice dsRNA (produced by annealing Ice^cIa and Ice^a.cIa RNA), Dcp-1 dsRNA (produced by annealing Dcp-1^cIa and Dcp-1^a.cIa RNA) or Ark dsRNA (produced by annealing Ark^cIa and Ark^a.cIa RNA).

(Igaki et al., 2002)

Organismal death in the embryo, caused by rpr^hs.PW when heat shock is applied at 5-10 hours after egg laying is suppressed by th^SL. th^SL increases the viability of rpr^hs.PW flies threefold.

(Lisi et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Expressed in SL2 cells and induced apoptosis is studied. Co-expressed with BacA\p35, Cele\ced-9 and Hsap\BCL2L1 to study prevention of apoptosis.

(Hisahara et al., 1998)

Expressed in S2 cells and used in cell death assays.

(Kanuka et al., 1999)

Carried in plasmid "pCaspeR-hs-reaper" and transfected into S2 cells.

(Igaki et al., 2002)

Carried in a plasmid and transfected into S2 cells as a control to observe cell death.

(Oshima, 2006)

Carried in plasmid "hs-rpr" and transfected into S2 cells to study the affect of the expressed protein activity on the viability of the cells.

(Kuranaga et al., 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

rpr^hs.PW

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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