Amino acid replacement: S421L.
Nucleotide substitution: C1614T.
Point mutation.
C3147525T
C1614T
S421L | Mad-PA; S491L | Mad-PB
S421L
Clonal analysis in the germarium reveals that mutant stem cells are lost more rapidly than wild type, though there is no effect on the formation of 16 cell cysts or their subsequent development. Stem cell half life is 2.5 weeks (wild type being 4.6 weeks). Stem cell division rate relative to control is 0.6. Cysts contain the normal 16 cells, including and oocyte.
Mad mutations can be placed in an allelic series based on relative severity of the maternal effect enhancement of weak dpp alleles: Mad1 < Mad7 < Mad12 < Mad10 < Mad5 < Mad3 < Mad2 < Mad11 < Mad6 < Mad4 < Mad8 < Mad9.