1.1kb fragment of the Hsp83 promoter (containing the ATG codon, translation start site and NLS) drives the expression of a 1.0kb PstI ClaI P\T fragment from Ecol\lacZP\T.1911-2150.hs (containing the third intron (IVS3) and flanking exons) which in turn is fused in frame to a 3.4kb ClaI XhoI fragment carrying the 3' half of Ecol\lacZ fused to Tn\neoR (Friedrich and Soriano, 1991, Genes Dev 5: 1513-1523).
Ectopic expression of Psi in the female germline inhibits splicing of an IVS3 containing reporter construct.