FB2024_03 , released June 25, 2024
Recombinant Construct: P{hsp83-IVS3-βgeo}
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General Information
Symbol
P{hsp83-IVS3-βgeo}
FlyBase ID
FBtp0005060
Feature type
transgenic_transposable_element
Component Allele(s)
P\TIVS3.Hsp83.βgeo
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
ry+t7.2
Component allele P\TIVS3.Hsp83.βgeo
Transgenic product class
Component
Use(s)
Regulatory region(s)
Hsp83
Encoded product / tool
P\T
Description

1.1kb fragment of the Hsp83 promoter (containing the ATG codon, translation start site and NLS) drives the expression of a 1.0kb PstI ClaI P\T fragment from Ecol\lacZP\T.1911-2150.hs (containing the third intron (IVS3) and flanking exons) which in turn is fused in frame to a 3.4kb ClaI XhoI fragment carrying the 3' half of Ecol\lacZ fused to Tn\neoR (Friedrich and Soriano, 1991, Genes Dev 5: 1513-1523).

(Roche et al., 1995)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
P{hsp83-IVS3-βgeo}
(FlyBase, 1996-)
hsp83-IVS3-LacZ
(Lo et al., 2016, Rio, 1999)
hsp83-IVS3-LacZ-neo
(Teixeira et al., 2017)
Secondary FlyBase IDs
    References (8)