FlyBase Allele Report: Dmel\Cyt-b5[k14202]

General Information

Symbol

Dmel\Cyt-b5^k14202

Species

D. melanogaster

Name

FlyBase ID

FBal0045503

Feature type

allele

Associated gene

Dmel\Cyt-b5

Associated Insertion(s)

P{lacW}Cyt-b5^k14202

Carried in Construct

Also Known As

dpld^EJL

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Kleinhesselink et al., 2011)

Mutagen

P-element activity

(Rodriguez et al., 1996, Wu et al., 1996)

Progenitor genotype

Associated Insertion(s)

P{lacW}Cyt-b5^k14202

Cytology

Description

Insertion within the 5' UTR of Cyt-b5, at position -19 relative to the translation start codon.

(Kleinhesselink et al., 2011)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:7,533,592..7,533,592

(Kleinhesselink et al., 2011, Spradling et al., 1999, Kimbrell, 1996.4.28, Rodriguez et al., 1996, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

hemocyte

(Kleinhesselink et al., 2011)

lamellocyte (with Cyt-b5^k09716)

(Kleinhesselink et al., 2011)

Detailed Description

Statement

Reference

Cyt-b5^k14202/Cyt-b5^k09716 females and males produce few progeny, even when mated with wild-type flies, and the mutant females lay few eggs.

Cyt-b5^k14202/Cyt-b5^k09716 larvae contain large numbers of circulating lamellocytes (these are not found in normal larvae). Lamellocytes are more prevalent in larvae containing small melanotic tumours, while plasmatocytes are more abundant in larvae containing large melanotic tumours.

The number of plasmatocytes from both hematopoietic waves are increased in heterozygous larvae of both sexes compared to wild type.

(Kleinhesselink et al., 2011)

Homozygous larvae exhibit melanotic tumours and aberrant fat body and gut.

(Rodriguez et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Cyt-b5^k09716/Cyt-b5^k14202 is rescued by Cyt-b5^UAS.cKa/Scer\GAL4^He.PZ

(Kleinhesselink et al., 2011)

Df(2R)ST1/Cyt-b5^k14202 is rescued by Scer\GAL4^da.G32/Cyt-b5^UAS.cKa

(Kleinhesselink et al., 2011)

Not rescued by

Cyt-b5^k09716/Cyt-b5^k14202 is not rescued by Cyt-b5^UAS.cKa/Scer\GAL4^Sgs3.PD

(Kleinhesselink et al., 2011)

Df(2R)ST1/Cyt-b5^k14202 is not rescued by Cyt-b5^UAS.cKa/Scer\GAL4^He.PZ

(Kleinhesselink et al., 2011)

Comments

The melanotic tumour phenotype seen in Cyt-b5^k14202/Cyt-b5^k09716 animals is rescued by expression of Cyt-b5^Scer\UAS.cKa under the control of Scer\GAL4^He.PZ, but is not rescued when expression is driven by Scer\GAL4^Sgs3.PD.

The lethality of Cyt-b5^k14202/Df(2R)ST1 animals is not rescued by expression of Cyt-b5^Scer\UAS.cKa under the control of Scer\GAL4^He.PZ, but is rescued when expression is driven by Scer\GAL4^da.G32.

(Kleinhesselink et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

102915

w¹¹¹⁸; P{lacW}Cyt-b5^k14202 / CyO

Notes on Origin

Discoverer

Comments

Complements: Aldh-III⁰³⁶¹⁰.

(BDGP Project Members, 1994-1999)

Mobilisation of P{lacW} reverts the lethal phenotype demonstrating the insertion causes the mutant phenotype. Level of Ecol\lacZ expression and viability of adults is not altered by bacterial infection of adults.

(Rodriguez et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

142/2

(Rodriguez et al., 1996)

Cyt-b5^k14202

dpld^EJL

(Kleinhesselink et al., 2011, Kimbrell, 1996.4.28, Rodriguez et al., 1996)

dpld^k14202

l(2)k14202

(Spradling et al., 1999)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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